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A Glycoside Flavonoid in Kudzu (Pueraria lobata)

Identification, Quantification, and Determination of Antioxidant Activity

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Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals

Part of the book series: ABAB Symposium ((ABAB))

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Abstract

Kudzu (Pueraria lobata) foliage has been touted as a possible energy crop. High-performance liquid chromatography and mass spectrometry analysis of the methanolic kudzu foliage extracts confirmed the presence of robinin (kaempferol-3-O-robinoside-7-O-rhamnoside). Robinin accounted for 0.65 ± 0.16% (dry basis) of kudzu biomass. Fast performance liquid chromatography (FPLC) was employed to fractionate robinin from the crude extract. The antioxidant capacity of robinin was evaluated by an oxygen radical absorbance capacity (ORAC) assay. The ORAC values of pure standard were compared with those of the extract fractions. One milligram of the FPLC-fractionated robinin generated an ORAC value of 5.15 ± 2.00 umol/mg of Trolox, whereas 1 mg of pure robinin generated an ORAC value of 12.34 ± 0.45 µmol/mg of Trolox. Because of its antioxidant properties, robinin may be a flavonoid worth extracting prior to energy production.

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, 3202 Bell Engineering Center, USA

    Ching S. Lau, Robert R. Beitle & Edgar C. Clausen

  2. Department of Biological and Agricultural Engineering, 203 Engineering Hall, USA

    Danielle J. Carrier

  3. Department of Food Science, 2650 N. Young Avenue, USA

    Luke R. Howard

  4. Department of Chemistry and Biochemistry, University of Arkansas, 115 Chemistry Building, Fayetteville, AR, 72701

    Jackson O. Lay Jr. & Rohana Liyanage

Authors
  1. Ching S. Lau
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  2. Danielle J. Carrier
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  3. Robert R. Beitle
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  4. Luke R. Howard
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  5. Jackson O. Lay Jr.
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  6. Rohana Liyanage
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  7. Edgar C. Clausen
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Editor information

Editors and Affiliations

  1. Oak Ridge National Laboratory, USA

    Brian H. Davison  & Barbara R. Evans  & 

  2. National Renewable Energy Laboratory, USA

    Mark Finkelstein  & James D. McMillan  & 

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© 2005 Humana Press Inc.

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Lau, C.S. et al. (2005). A Glycoside Flavonoid in Kudzu (Pueraria lobata). In: Davison, B.H., Evans, B.R., Finkelstein, M., McMillan, J.D. (eds) Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-59259-991-2_66

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