Chemistry and Analysis of Phytocannabinoids and Other Cannabis Constituents

Part of the Forensic Science And Medicine book series (FSM)


The Cannabis plant and its products consist of an enormous variety of chemicals. Some of the 483 compounds identified are unique to Cannabis, for example, the more than 60 cannabinoids, whereas the terpenes, with about 140 members forming the most abundant class, are widespread in the plant kingdom. The term “cannabinoids” [note: “ ” represents a group of C21 terpenophenolic compounds found until now uniquely in Cannabis sativa L. (1). As a consequence of the development of synthetic cannabinoids (e.g., nabilone [2], HU-211 [dexanabinol; ref. (3), or ajulemic acid [CT-3; ref. 4]) and the discovery of the chemically different endogenous cannabinoid receptor ligands (“endocannabinoids,” e.g., anandamide, 2-arachidonoylglycerol) (5,6), the term ’“phytocannabinoids’” was proposed for these particular Cannabis constituents (7).


High Pressure Liquid Chromatography Cannabis Sativa Cannabis Plant Cannabis Product Cannabis Resin 
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  1. 1.
    Mechoulam, R. and Gaoni, Y. (1967) Recent advances in the chemistry of hashish. Fortschr. Chem. Org. Naturst. 25, 175–213.PubMedGoogle Scholar
  2. 2.
    Ward, A. and Holmes, B. (1985) Nabilone. A preliminary review of its pharmacological properties and therapeutic use. Drugs 30, 127–144.PubMedGoogle Scholar
  3. 3.
    Mechoulam, R., Lander, N., Breuer, A., and Zahalka, J. (1990) Synthesis of the individual, pharmacologically distinct, enantiomers of a tetrahydrocannabinol derivative. Tetrahedron Asymmetry 1, 315–318.CrossRefGoogle Scholar
  4. 4.
    Burstein, S. H., Audette, C. A., Breuer, A., et al. (1992) Synthetic nonpsychotropic cannabinoids with potent antiinflammatory, analgesic, and leukocyte antiadhesion activities. J. Med. Chem. 35, 3135–3141.PubMedCrossRefGoogle Scholar
  5. 5.
    Di Marzo, V. and Fontana, A. (1995) Anandamide, an endogenous cannabinomimetic eicosanoid:’ killing two birds with one stone’. Prostaglandins Leukot. Essent. Fatty Acids 53, 1–11.PubMedCrossRefGoogle Scholar
  6. 6.
    Devane, W. A., Hanus, L., Breuer, A., et al. (1992) Isolation and structure of a brain constituent that binds to the cannabinoid receptor. Science 258, 1946–1949.PubMedCrossRefGoogle Scholar
  7. 7.
    Pate, D. (1999) Anandamide structure-activity relationships and mechanisms of action on intraocular pressure in the normotensive rabbit model, PhD thesis, University of Kuopio, Kuopio, Finland.Google Scholar
  8. 8.
    Turner, C. E., Elsohly, M. A., and Boeren, E. G. (1980) Constituents of Cannabis sativa L. XVII. A review of the natural constituents. J. Nat. Prod. 43, 169–234.PubMedCrossRefGoogle Scholar
  9. 9.
    Ross, S. A. and ElSohly, M. A. (1995) Constituents of Cannabis sativa L. XXVIII-A review of the natural constituents: 1980-1994. Zagazig J. Pharm. Sci. 4, 1–10.Google Scholar
  10. 10.
    ElSohly, M. (2002) Chemical constituents of Cannabis, in Cannabis and cannabinoids— Pharmacology, Toxicology and Therapeutic Potential (Grotenhermen, F. and Russo, E., eds.), Haworth Press, New York, pp. 27–36.Google Scholar
  11. 11.
    Gaoni, Y. and Mechoulam, R. (1964) The structure and synthesis of cannabigerol, a new hashish constituent, in Proc. Chem. Soc., London, p. 82.Google Scholar
  12. 12.
    Shoyama, Y., Yagi, M., Nishioka, I., and Yamauchi, T. (1975) Biosynthesis of cannab-inoid acids. Phytochemistry 14, 2189–2192.CrossRefGoogle Scholar
  13. 13.
    Adams, R., Hunt, M., and Clark, J. (1940) Structure of cannabidiol, a product isolated from the marihuana extract of Minnesota wild hemp. I. J. Am. Chem. Soc. 62, 196–199.CrossRefGoogle Scholar
  14. 14.
    Mechoulam, R. and Shvo, Y. (1963) Hashish—I. The structure of cannabidiol. Tetrahedron 19, 2073–2078.CrossRefGoogle Scholar
  15. 15.
    Wollner, H., Matchett, J., Levine, J., and Loewe, S. (1942) Isolation of a physiologically active tetrahydrocannabinol from Cannabis sativa resin. J. Am. Chem. Soc. 64, 26–29.CrossRefGoogle Scholar
  16. 16.
    Gaoni, Y. and Mechoulam, R. (1964) Isolation, structure and partial synthesis of an active constituent of hashish. J. Am. Chem. Soc. 86, 1646–1647.CrossRefGoogle Scholar
  17. 17.
    Wood, T., Spivey, W., and Easterfield, T. (1896) XL. Charas. The resin of Indian hemp. J. Chem. Soc. 69, 539.Google Scholar
  18. 18.
    Adams, R., Baker, B., and Wearn, R. (1940) Structure of cannabinol III. Synthesis of cannabinol, 1-hydroxy-3-n-amyl-6,6,9-trimethyl-6-dibenzopyran. J. Am. Chem. Soc. 62, 2204–2207.CrossRefGoogle Scholar
  19. 19.
    ElSohly, M., Ross, S., Mehmedic, Z., Arafat, R., Yi, B., and Banahan, B.F., 3rd (2000) Potency trends of delta-9-THC and other cannabinoids in confiscated marijuana from 1980-1997. J. Forens. Sci. 45, 24–30.Google Scholar
  20. 20.
    Brenneisen, R. (1986) The cannabinoid content in Cannabis products confiscated in Switzerland. Arch. Kriminol. 177, 95–104.Google Scholar
  21. 21.
    Brenneisen, R. and Meyer, P. Swiss Cannabis Profiling Project, University of Bern and Swiss Federal Office of Public Health (unpublished data).Google Scholar
  22. 22.
    Miller Coyle, H., Palmbach, T., Juliano, N., Ladd, C., and Lee, H. C. (2003) An overview of DNA methods for the identification and individualization of marijuana. Croat. Med. J. 44, 315–321.PubMedGoogle Scholar
  23. 23.
    ElSohly, M. (2003) Practical challenges to positive drug tests for marijuana. Clin. Chem. 49, 1037–1038.PubMedCrossRefGoogle Scholar
  24. 24.
    Leson, G., Pless, P., Grotenhermen, F., Kalant, H., and ElSohly, M. A. (2001) Evaluating the impact of hemp food consumption on workplace drug tests. J. Anal. Toxicol. 25, 691–698.PubMedGoogle Scholar
  25. 25.
    Ross, S. A., Mehmedic, Z., Murphy, T. P., and Elsohly, M. A. (2000) GC-MS analysis of the total delta9-THC content of both drug-and fiber-type Cannabis seeds. J. Anal. Toxicol. 24, 715–717.PubMedGoogle Scholar
  26. 26.
    Bosy, T. Z. and Cole, K. A. (2000) Consumption and quantitation of delta-9-tetrahydrocan-nabinol in commercially available hemp seed oil products. J. Anal. Toxicol. 24, 562–6.PubMedGoogle Scholar
  27. 27.
    Mediavilla, V., Derungs, R., Känzig, A., and Mägert, A. (1997) Qualität von Hanfsamenöl aus der Schweiz. Agrarforschung 4, 449–451.Google Scholar
  28. 28.
    Lehmann, T., Sager, F., and Brenneisen, R. (1997) Excretion of cannabinoids in urine after ingestion of Cannabis seed oil. J. Anal. Toxicol. 21, 373–375.PubMedGoogle Scholar
  29. 29.
    Mediavilla, V. and Steinemann, S. (1997) Essential oil of Cannabis sativa L. strains. J. Int. Hemp Assoc. 4, 80–82.Google Scholar
  30. 30.
    Meier, C. and Mediavilla, V. (1998) Factors influencing the yield and the quality of hemp (Cannabis sativa L.) essential oil. J. Int. Hemp Assoc. 5, 16–20.Google Scholar
  31. 31.
    Lehmann, T. (1995) Chemical profiling of Cannabis sativa L., PhD thesis, University of Bern, Dep. of Pharmaceutical Sciences, Bern, Switzerland.Google Scholar
  32. 32.
    Ross, S.A. and ElSohly, M. A. (1996) The volatile oil composition of fresh and air-dried buds of Cannabis sativa. J. Nat. Prod. 59, 49–51.PubMedCrossRefGoogle Scholar
  33. 33.
    Novak, J., Zitterl-Eglseer, K., Deans, S. G., and Franz, C. M. (2001) Essential oils of different cultivars of Cannabis sativa L. and their antimicrobial activity. Flavour Fragr. J. 16, 259–262.CrossRefGoogle Scholar
  34. 34.
    McPartland, J. M. and Russo, E. B. (2001) Cannabis and Cannabis extracts: greater than the sum of their parts? J. Cann. Therap. 1, 103–132.CrossRefGoogle Scholar
  35. 35.
    Hendriks, H., Malingré, T. M., Batterman, S., and Bos, R. (1977) Alkanes of the essential oil of Cannabis sativa. Phytochemistry 16, 719–721.CrossRefGoogle Scholar
  36. 36.
    McPartland, J. and Mediavilla, V. (2002) Noncannabinoid components, in Cannabis and Cannabinoids-Pharmacology, Toxicology, and Therapeutic Potential (Grotenhermen, F., and Russo, E., eds.), Haworth Press, New York, pp. 401–409.Google Scholar
  37. 37.
    Vanhoenacker, G., Van Rompaey, P., De Keukeleire, D., and Sandra, P. (2002) Chemo-taxonomic features associated with flavonoids of cannabinoid-free Cannabis (Cannabis sativa subsp. sativa L.) in relation to hops (Humulus lupulus L.). Nat. Prod. Lett. 16, 57–63.PubMedCrossRefGoogle Scholar
  38. 38.
    Barrett, M. L., Scutt, A. M., and Evans, F. J. (1986) Cannflavin A and B, prenylated flavones from Cannabis sativa L. Experientia 42, 452–453.PubMedCrossRefGoogle Scholar
  39. 39.
    Barrett, M. L., Gordon, D., and Evans, F. J. (1985) Isolation from Cannabis sativa L. of cannflavin-a novel inhibitor of prostaglandin production. Biochem. Pharmacol. 34, 2019–2024.PubMedCrossRefGoogle Scholar
  40. 40.
    Leson, G., Pless, P., and Roulac, J. (1999) Hemp Foods and Oils for Health, Hemptech, Sebastopol, CA.Google Scholar
  41. 41.
    Ross, S., ElSohly, H., ElKashoury, E., and ElSohly, M. (1996) Fatty acids of Cannabis seeds. Phytochem. Anal. 7, 279–283.CrossRefGoogle Scholar
  42. 42.
    Grotenhermen, F. (2002) Effects of Cannabis and the cannabinoids, in Cannabis and Cannabinoids-Pharmacology, Toxicology, and Therapeutic Potential (Grotenhermen, F. and Russo, E., eds.), Haworth Press, New York, pp. 55–65.Google Scholar
  43. 43.
    Grotenhermen, F. and Russo, E. (eds.) (2002) Cannabis and Cannabinoids-Pharmacology, Toxicology, and Therapeutic Potential, Haworth Press, New York, p. 439.Google Scholar
  44. 44.
    Iversen, L. (2003) Cannabis and the brain. Brain 126, 1252–1270.PubMedCrossRefGoogle Scholar
  45. 45.
    Croxford, J. L. (2003) Therapeutic potential of cannabinoids in CNS disease. CNS Drugs 17, 179–202.PubMedCrossRefGoogle Scholar
  46. 46.
    Kumar, R., Chambers, W., and Pertwee, R. G. (2001) Pharmacological actions and therapeutic uses of Cannabis and cannabinoids. Anaesthesia 56, 1059–68.PubMedCrossRefGoogle Scholar
  47. 47.
    Hirst, R. A., Lambert, D. G., and Notcutt, W. G. (1998) Pharmacology and potential therapeutic uses of Cannabis. Br. J. Anaesth. 81, 77–84.PubMedGoogle Scholar
  48. 48.
    Ashton, C. H. (1999) Adverse effects of Cannabis and cannabinoids. Br. J. Anaesth. 83, 637–49.PubMedGoogle Scholar
  49. 49.
    Williamson, E. M. and Evans, F. J. (2000) Cannabinoids in clinical practice. Drugs 60, 1303–1314.PubMedCrossRefGoogle Scholar
  50. 50.
    Campbell, F. A., Tramèr, M. R., Carroll, D., Reynolds, D. J., Moore, R. A., and McQuay, H. J. (2001) Are cannabinoids an effective and safe treatment option in the management of pain? A qualitative systematic review. Br. Med. J. 323, 13–16.CrossRefGoogle Scholar
  51. 51.
    .Tramèr, M. R., Carroll, D., Campbell, F. A., Reynolds, D. J., Moore, R. A., and McQuay, H. J. (2001) Cannabinoids for control of chemotherapy induced nausea and vomiting: quantitative systematic review. Br. Med. J. 323, 16–21.CrossRefGoogle Scholar
  52. 52.
    Walker, J. M. and Huang, S. M. (2002) Cannabinoid analgesia. Pharmacol. Ther. 95, 127–135.PubMedCrossRefGoogle Scholar
  53. 53.
    Voth, E. A. and Schwartz, R. H. (1997) Medicinal applications of delta-9-tetrahydrocan-nabinol and marijuana. Ann. Int. Med. 126, 791–798.PubMedGoogle Scholar
  54. 54.
    McPartland, J. M. and Pruitt, P. L. (1999) Side effects of pharmaceuticals not elicited by comparable herbal medicines: the case of tetrahydrocannabinol and marijuana. Altern. Ther. 5, 57–62.CrossRefGoogle Scholar
  55. 55.
    Wachtel, S. R., ElSohly, M. A., Ross, S. A., Ambre, J., and de Wit, H. (2002) Comparison of the subjective effects of delta-9-tetrahydrocannabinol and marijuana in humans. Psychopharmacology 161, 331–339.PubMedCrossRefGoogle Scholar
  56. 56.
    Hart, C. L., Ward, A. S., Haney, M., Comer, S. D., Foltin, R. W., and Fischman, M. W. (2002) Comparison of smoked marijuana and oral Delta(9)-tetrahydrocannabinol in humans. Psychopharmacology 164, 407–415.PubMedCrossRefGoogle Scholar
  57. 57.
    Mechoulam, R., Parker, L. A., and Gallily, R. (2002) Cannabidiol: an overview of some pharmacological aspects. J. Clin. Pharmacol. 42, 11S–19S.PubMedGoogle Scholar
  58. 58.
    Consroe, P. (1998) Brain cannabinoid systems as targets for the therapy of neurological disorders. Neurobiol. Dis. 5, 534–551.PubMedCrossRefGoogle Scholar
  59. 59.
    Bornheim, L. M., Kim, K. Y., Li, J., Perotti, B. Y., and Benet, L. Z. (1995) Effect of cannabidiol pretreatment on the kinetics of tetrahydrocannabinol metabolites in mouse brain. Drug Metab. Dispos. 23, 825–831.PubMedGoogle Scholar
  60. 60.
    Meschler, J. P. and Howlett, A. C. (1999) Thujone exhibits low affinity for cannabinoid receptors but fails to evoke cannabimimetic responses. Pharmacol. Biochem. Behav. 62, 473–480.PubMedCrossRefGoogle Scholar
  61. 61.
    Russo, E. (2001) Hemp for headache: an in-depth historical and scientific review of can-nabis in migraine treatment. J. Cann. Ther. 1, 21–92.CrossRefGoogle Scholar
  62. 62.
    Salgueiro, J. B., Ardenghi, P., Dias, M., Ferreira, M. B., Izquierdo, I., and Medina, J. H. (1997) Anxiolytic natural and synthetic flavonoid ligands of the central benzodiazepine receptor have no effect on memory tasks in rats. Pharmacol. Biochem. Behav. 58, 887–891.PubMedCrossRefGoogle Scholar
  63. 63.
    Mitosinka, G. T., Thornton, J. I., and Hayes, T. L. (1972) The examination of cystolithic hairs of Cannabis and other plants by means of the scanning electron microscope. J. Forens. Sci Soc. 12, 521–529.Google Scholar
  64. 64.
    Thornton, J. I. and Nakamura, G. R. (1972) The identification of marijuana. J. Forens. Sci. Soc. 12, 461–519.Google Scholar
  65. 65.
    Gigliano, G. (2001) Cannabis sativa L.—botanical problems and molecular approaches in forensic investigations. Forens. Sci. Rev. 13, 2–17.Google Scholar
  66. 66.
    .Stearn, W. T. (1970) The Cannabis plant: botanical characteristics, in The Botany & Chemistry of Cannabis (Joyce, C. and Curry, S., eds.), J. & A. Churchill, London, p. 1.Google Scholar
  67. 67.
    Nordal, A. (1970) Microscopic detection of Cannabis in the pure state and in semi-combusted residues, in The Botany & Chemistry of Cannabis (Joyce, C. and Curry, S., eds.), J. & A. Churchill, London, pp. 61–68.Google Scholar
  68. 68.
    Petri, G., Oroszlan, P., and Fridvalszky, L. (1988) Histochemical detection of hemp tri-chomes and their correlation with the THC content. Acta Biol. Hung. 39, 59–73.PubMedGoogle Scholar
  69. 69.
    Bruni, A., Barni Comparini, I., and Menziani Andreoli, E. (1983) A histofluorescent procedure for identifying marijuana cannabinoids. Experientia 39, 886–888.PubMedCrossRefGoogle Scholar
  70. 70.
    United Nations (1987) Recommended Methods for Testing Cannabis, ST/NAR/8, Division of Narcotic Drugs, United Nations, New York.Google Scholar
  71. 71.
    Bailey, K. (1979) The value of the Duquenois test for Cannabis-a survey. J. Forens. Sci. 24, 817–841.Google Scholar
  72. 72.
    Butler, W. (1962) Duquenois-Levine test for marijuana. J. Assoc. Off. Anal. Chem. 45, 597–600.Google Scholar
  73. 73.
    Tewari, S. N. and Sharma, J. D. (1982) Spot tests for Cannabis materials. Bull. Narc. 34, 109–112.PubMedGoogle Scholar
  74. 74.
    Pitt, C. G., Hendron, R. W., and Hsia, R. S. (1972) The specificity of the Duquenois color test for marihuana and hashish. J. Forens. Sci. 17, 693–700.Google Scholar
  75. 75.
    Mali, B. D. and Parulekar, P. P. (1988) Diazotized dapsone as a reagent for the detection of cannabinoids on thin-layer chromatographic plates. J. Chromatogr. 457, 383–386.PubMedCrossRefGoogle Scholar
  76. 76.
    Baker, P. B., Gough, T. A., and Taylor, B. J. (1980) Illicitly imported Cannabis products: some physical and chemical features indicative of their origin. Bull. Narc. 32, 31–40.PubMedGoogle Scholar
  77. 77.
    Debruyne, D., Albessard, F., Bigot, M. C., and Moulin, M. (1994) Comparison of three advanced chromatographic techniques for Cannabis identification. Bull. Narc. 46, 109–121.PubMedGoogle Scholar
  78. 78.
    Pothier, J., Galand, N., and Viel, C. (1992) Rapid characterization of stupefacient and toxic substances by pressurized thin-layer chromatography. J. Toxicol. Clin. Exp. 12, 495–501.PubMedGoogle Scholar
  79. 79.
    Oroszlan, P., Verzar-Petri, G., Mincsovics, E., and Szekely, T. (1987) Separation, quantitation and isolation of cannabinoids from Cannabis sativa L. by overpressured layer chromatography. J. Chromatogr. 388, 217–224.PubMedCrossRefGoogle Scholar
  80. 80.
    Ferioli, V., Rustichelli, C., Pavesi, G., and Gamberini, G. (2000) Analytical characterisation of hashish samples. Chromatographia 52, 39–44.CrossRefGoogle Scholar
  81. 81.
    Debruyne, D., Moulin, M., Bigot, M. C., and Camsonne, R. (1981) Identification and differentiation of resinous Cannabis and textile Cannabis: combined use of HPLC and high-resolution GLC. Bull. Narc. 33, 49–58.PubMedGoogle Scholar
  82. 82.
    Tsatsakis, A. M., Tutudaki, M., Stiakakis, I., Dimopoulou, M., Tzatzarakis, M., and Michalodimitrakis, M. (2000) Characterisation of Cannabis plants phenotypes from illegal cultivations in Crete. Boll. Chim. Farm. 139, 140–145.PubMedGoogle Scholar
  83. 83.
    Ross, S. A. and ElSohly, M. A. (1996) The volatile oil composition of fresh and air-dried buds of Cannabis sativa. J. Nat. Prod. 59, 49–51.PubMedCrossRefGoogle Scholar
  84. 84.
    Barni Comparini, I. and Centini, F. (1983) Packed column chromatography, high-resolution gas-chromatography and high pressure liquid chromatography in comparison for the analysis of Cannabis constituents. Forens. Sci. Int. 21, 129–137.CrossRefGoogle Scholar
  85. 85.
    Harvey, D. J. (1990) Stability of cannabinoids in dried samples of Cannabis dating from around 1896-1905. J. Ethnopharmacol. 28, 117–128.PubMedCrossRefGoogle Scholar
  86. 86.
    Turner, C. E., Bouwsma, O. J., Billets, S., and Elsohly, M. A. (1980) Constituents of Cannabis sativa L. XVIII-Electron voltage selected ion monitoring study of cannabinoids. Biomed. Mass Spectrom. 7, 247–256.PubMedCrossRefGoogle Scholar
  87. 87.
    Brenneisen, R. and ElSohly, M. A. (1988) Chromatographic and spectroscopic profiles of Cannabis of different origins: PartI. J. Forens. Sci. 33, 1385–1404.Google Scholar
  88. 88.
    Bosy, T. Z. and Cole, K. A. (2000) Consumption and quantitation of delta-9-tetrahydrocan-nabinol in commercially available hemp seed oil products. J. Anal. Toxicol. 24, 562–566.PubMedGoogle Scholar
  89. 89.
    Lercker, G., Bocci, F., Frega, N., and Bortolomeazzi, R. (1992) Cannabinoid acids analysis. Farmaco 47, 367–378.PubMedGoogle Scholar
  90. 90.
    Vree, T. B. (1977) Mass spectrometry of cannabinoids. J. Pharm. Sci. 66, 1444–1450.PubMedCrossRefGoogle Scholar
  91. 91.
    Novotny, M., Lee, M. L., Low, C. E., and Raymond, A. (1976) Analysis of marijuana samples from different origins by high-resolution gas-liquid chromatography for forensic application. Anal. Chem. 48, 24–29.PubMedCrossRefGoogle Scholar
  92. 92.
    Raharjo, T. J. and Verpoorte, R. (2004) Methods for the analysis of cannabinoids in biological materials: a review. Phytochem. Anal. 15, 79–94.PubMedCrossRefGoogle Scholar
  93. 93.
    Lehmann, T. and Brenneisen, R. (1995) High performance liquid chromatographic profiling of Cannabis products. J. Liq. Chromatogr. 18, 689–700.CrossRefGoogle Scholar
  94. 94.
    Barni Comparini, I. and Centini, F. (1983) Packed column chromatography, high-resolution gas-chromatography and high pressure liquid chromatography in comparison for the analysis of Cannabis constituents. Forens. Sci. Int. 21, 129–37.CrossRefGoogle Scholar
  95. 95.
    Zoller, O., Rhyn, P., and Zimmerli, B. (2000) High-performance liquid chromatographic determination of delta9-tetrahydrocannabinol and the corresponding acid in hemp containing foods with special regard to the fluorescence properties of delta9-tetrahydrocan-nabinol. J. Chromatogr. A 872, 101–110.PubMedCrossRefGoogle Scholar
  96. 96.
    Baker, P. B., Gough, T. A., and Wagstaffe, P. J. (1983) Determination of the distribution of cannabinoids in Cannabis resin from Morocco using high-performance liquid chroma-tography. Part II. J. Anal. Toxicol. 7, 7–10.PubMedGoogle Scholar
  97. 97.
    Baker, P. B., Taylor, B. J., and Gough, T. A. (1981) The tetrahydrocannabinol and tetrahydrocannabinolic acid content of Cannabis products. J. Pharm. Pharmacol. 33, 369–372.PubMedGoogle Scholar
  98. 98.
    McDonald, P. A. and Gough, T. A. (1984) Determination of the distribution of cannabinoids in Cannabis resin from the Lebanon using HPLC. Part III. J. Chromatogr. Sci. 22, 282–284.PubMedGoogle Scholar
  99. 99.
    Brenneisen, R. (1984) Psychotropic drugs. II. Determination of cannabinoids in Cannabis sativa L. and in Cannabis products with high pressure liquid chromatography (HPLC). Pharm. Acta Helv. 59, 247–259.PubMedGoogle Scholar
  100. 100.
    Rustichelli, C., Ferioli, V., Baraldi, M., Zanoli, P., and Gamberini, G. (1998) Analysis of cannabinoids in fiber hemp plant varieties (Cannabis sativa) by high-performance liquid chromatography. Chromatographia 8, 215–222.CrossRefGoogle Scholar
  101. 101.
    Baker, P. B., Fowler, R., Bagon, K. R., and Gough, T. A. (1980) Determination of the distribution of cannabinoids in Cannabis resin using high performance liquid chromatography. J. Anal. Toxicol. 4, 145–152.PubMedGoogle Scholar
  102. 102.
    Nakahara, Y. and Tanaka, K. (1988) Studies on discrimination of confiscated Cannabis products by high performance liquid chromatography with electrochemical detector. Eisei Shikenjo Hokoku, Bulletin of National Institute of Hygenic Sciences, pp.11–18.Google Scholar
  103. 103.
    Rustichelli, C., Ferioli, V., Vezzalini, F., Rossi, M. C., and Gamberini, G. (1996) Simultaneous separation and identification of hashish constituents by coupled liquid chroma-tography-mass spectrometry (HPLC-MS). Chromatographia 43, 129–134.CrossRefGoogle Scholar
  104. 104.
    Lurie, I. S., Meyers, R. P., and Conver, T. S. (1998) Capillary electrochromatography of cannabinoids. Anal. Chem. 70, 3255–3260.PubMedCrossRefGoogle Scholar
  105. 105.
    Backstrom, B., Cole, M. D., Carrott, M. J., Jones, D. C., Davidson, G., and Coleman, K. (1997) A preliminary study of the analysis of Cannabis by supercritical fluid chromatography with atmospheric pressure chemical ionisation mass spectroscopic detection. Sci. Justice 37, 91–97.PubMedGoogle Scholar
  106. 106.
    Miller Coyle, H., Ladd, C., Palmbach, T., and Lee, H. C. (2001) The green revolution: botanical contributions to forensics and drug enforcement. Croat. Med. J. 42, 340–345.PubMedGoogle Scholar
  107. 107.
    Miller Coyle, H., Palmbach, T., Juliano, N., Ladd, C., and Lee, H. C. (2003) An overview of DNA methods for the identification and individualization of marijuana. Croat. Med. J. 44, 315–321.PubMedGoogle Scholar
  108. 108.
    Cole, M. D. and Linacre, A. M. T. (2002) The identification of controlled plant drugs using phytochemistry and DNA. Curr. Topics Phytochem. 5, 129–140.Google Scholar
  109. 109.
    Linacre, A. and Thorpe, J. (1998) Detection and identification of Cannabis by DNA. Forens. Sci. Int. 91, 71–76.CrossRefGoogle Scholar
  110. 110.
    Siniscalco Gigliano, G., Caputo, P., and Cozzolino, S. (1997) Ribosomal DNA analysis as a tool for the identification of Cannabis sativa L. specimens of forensic interest. Sci. Justice 37, 171–174.PubMedGoogle Scholar
  111. 111.
    Gillan, R., Cole, M., Linacre, A., Thorpe, J. W., and Watson, N. D. (1995) Comparison of Cannabis sativa by random amplification of polymorphic DNA (RAPD) and HPLC of cannabinoids: a preliminary study. Sci. Justice 35, 169–177.PubMedGoogle Scholar
  112. 112.
    Miller Coyle, H., Sutler, G., Abrams, S., et al. (2003) A simple DNA extraction method for Marijuana samples used in amplified fragment length polymorphism (AFLP) analysis. J. Forens. Sci. 48, 343–347.Google Scholar
  113. 113.
    Hsieh, H. M., Hou, R. J., Tsai, L. C., et al. (2003) A highly polymorphic STR locus in Cannabis sativa. Forens. Sci. Int. 131, 53–58.CrossRefGoogle Scholar
  114. 114.
    Gilmore, S., Peakall, R., and Robertson, J. (2003) Short tandem repeat (STR) DNA markers are hypervariable and informative in Cannabis sativa: implications for forensic investigations. Forens. Sci. Int. 131, 65–74.CrossRefGoogle Scholar
  115. 115.
    Kojoma, M., Iida, O., Makino, Y., Sekita, S., and Satake, M. (2002) DNA fingerprinting of Cannabis sativa using inter-simple sequence repeat (ISSR) amplification. Planta Med. 68, 60–63.PubMedCrossRefGoogle Scholar
  116. 116.
    Gigliano, G. (1998) Identification of Cannabis sativa L. (Cannabaceae) using restriction profiles of the Internal Transcribed Spacer II (ITS2). Sci. Justice 38, 225–230.PubMedCrossRefGoogle Scholar
  117. 117.
    Alghanim, H. J. and Almirall, J. R. (2003) Development of micro satellite markers in Cannabis sativa for DNA typing and genetic relatedness analyses. Anal. Bioanal. Chem. 376, 1225–1233.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, New Jersey 2007

Authors and Affiliations

  1. 1.Department of Clinical Research, Laboratory for Phytopharmacology, Bioanalytics and PharmacokineticsUniversity of BernBernSwitzerland

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