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Optimization of Brewery’s Spent Grain Dilute-Acid Hydrolysis for the Production of Pentose-Rich Culture Media

  • Conference paper
Proceedings of the Twenty-Fifth Symposium on Biotechnology for Fuels and Chemicals Held May 4–7, 2003, in Breckenridge, CO

Part of the book series: Biotechnology for Fuels and Chemicals ((ABAB))

Abstract

Dilute-acid hydrolysis of brewery’s spent grain to obtain a pentose-rich fermentable hydrolysate was investigated. The influence of operational conditions on polysaccharide hydrolysis was assessed by the combined severity parameter (CS) in the range of 1.39–3.06. When the CS increased, the pentose sugars concentration increased to a maximum at a CS of 1.94, whereas the maximum glucose concentration was obtained for a CS of 2.65. The concentrations of furfural, hydroxymethylfurfural (HMF), as well as formic and levulinic acids and total phenolic compounds increased with severity. Optimum hydrolysis conditions were found at a CS of 1.94 with >95% of feedstock pentose sugars recovered in the monomeric form, together with a low content of furfural, HMF, acetic and formic acids, and total phenolic compounds. This hydrolysate containing glucose, xylose, and arabinose (ratio 10:67:32) was further supplemented with inorganic salts and vitamins and readily fermented by the yeast Debaryomyces hansenii CCMI 941 without any previous detoxification stage. The yeast was able to consume all sugars, furfural, HMF, and acetic acid with high biomass yield, 0.68 C-mol/C-mol, and productivity, 0.92 g/ (L.h). Detoxification with activated charcoal resulted in a similar biomass yield and a slight increase in the volumetric productivity (11%).

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Authors and Affiliations

  1. Departamento de Biotecnologia, INETI, Estrada do Paço do Lumiar 22, 1649-038, Lisboa, Portugal

    Florbela Carvalheiro, Luís C. Duarte, Raquel Medeiros & Francisco M. Gírio

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  1. Florbela Carvalheiro
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  2. Luís C. Duarte
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  3. Raquel Medeiros
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  4. Francisco M. Gírio
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Editors and Affiliations

  1. National Renewable Energy Laboratory, USA

    Mark Finkelstein  & James D. McMillan  & 

  2. Oak Ridge National Laboratory, USA

    Brian H. Davison  & Barbara Evans  & 

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Carvalheiro, F., Duarte, L.C., Medeiros, R., Gírio, F.M. (2004). Optimization of Brewery’s Spent Grain Dilute-Acid Hydrolysis for the Production of Pentose-Rich Culture Media. In: Finkelstein, M., McMillan, J.D., Davison, B.H., Evans, B. (eds) Proceedings of the Twenty-Fifth Symposium on Biotechnology for Fuels and Chemicals Held May 4–7, 2003, in Breckenridge, CO. Biotechnology for Fuels and Chemicals. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-837-3_86

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  • DOI: https://doi.org/10.1007/978-1-59259-837-3_86

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4684-9873-8

  • Online ISBN: 978-1-59259-837-3

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