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Study on Methane Fermentation and Production of Vitamin B12 from Alcohol Waste Slurry

  • Conference paper
Proceedings of the Twenty-Fifth Symposium on Biotechnology for Fuels and Chemicals Held May 4–7, 2003, in Breckenridge, CO

Part of the book series: Biotechnology for Fuels and Chemicals ((ABAB))

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Abstract

We studied biogas fermentation from alcohol waste fluid to evaluate the anaerobic digestion process and the production of vitamin B12 asa byproduct. Anaerobic digestion using acclimated methanogens was performed using the continuously stirred tank reactor (CSTR) and fixed-bed reactor packed with rock wool as carrier material at 55°C. We also studied the effects of metal ions added to the culture broth on methane and vitamin B12 formation. Vitamin B12 productionwas 2.92 mg/L in the broth of the fixed-bed reactor, twice that of the CSTR. The optimum concentrations of trace metal ions added to the culture liquid for methane and vitamin B12 production were 1.0 and 8 mL/L for the CSTR and fixed-bed reactor, respectively. Furthermore, an effective method for extracting and purifying vitamin B12 from digested fluid was developed.

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References

  1. Hill, D. T., Cobb, S. A., and Bolte, J. P. (1987), Trans. ASAE 30, 496–501.

    CAS  Google Scholar 

  2. Marchaim, U. and Krause, C. (1993), Bioresour. Technol. 43, 195–203.

    Article  CAS  Google Scholar 

  3. Zhang, Z. Y., Yang, Y. N., Lu, J., and Maekawa, T. (2001), Determination of extracelluar vitamin B12 compounds in anaerobic microbial, in Environment Protection and Technology. China Ocean Publishing Company, 69–80.

    Google Scholar 

  4. Christof, H., Martin, W., Karl-Heinz, R., and Georg, M. G. (2002), Bioresour. Technol. 81, 19–24.

    Article  Google Scholar 

  5. Andersson, J. and Björnsson, L. (2002), Bioresour. Technol. 85, 51–56.

    Article  PubMed  CAS  Google Scholar 

  6. Mazumder, T.K., Fukuzaki, S., and Nagai, S. (1987), Appl. Microbiol. Biotechnol. 26, 511–516.

    Article  CAS  Google Scholar 

  7. Chanto, A. Q., Meyer, A. C., Fucher, S., Jonas, M. F., Koechtopp, P., and Jonas R. (1998), Biotechnol. Tech. 12(1), 75–77.

    Article  Google Scholar 

  8. Sato, K. (1983), Vitamins (Japan), 57(11), 609–616.

    CAS  Google Scholar 

  9. Mazumder T. K., Nishio N., and Nagai, S. (1986), Biotechnol. Lett. 8(12), 643–648.

    Article  Google Scholar 

  10. Kenealy, W. R. and Zeikus, J. G. (1981), J. Bacteriol. 146, 133.

    PubMed  CAS  Google Scholar 

  11. Kida, K., Shigematsu, T., Kijima, J., Numaguchi, M., Mochinaga, Y., Abe, N., and Morimura, S. (2001), J. Biosci. Bioeng. 91(6), 590–595.

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Institute of Agricultural and Forest Engineering, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8573, Japan

    Zhenya Zhang, Norio Sugiura & Takaaki Maekawa

  2. Master Program of Biosystem Studies, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8573, Japan

    Taisheng Quan

  3. Doctoral Program of Agricultural Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8573, Japan

    Pomin Li

  4. Water Conservancy and Civil Engineering College, China Agricultural University, Peking, Haidian District, Qinhua Dong Road, China

    Yansheng Zhang

Authors
  1. Zhenya Zhang
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  2. Taisheng Quan
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  3. Pomin Li
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  4. Yansheng Zhang
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  5. Norio Sugiura
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  6. Takaaki Maekawa
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Editor information

Editors and Affiliations

  1. National Renewable Energy Laboratory, USA

    Mark Finkelstein  & James D. McMillan  & 

  2. Oak Ridge National Laboratory, USA

    Brian H. Davison  & Barbara Evans  & 

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© 2004 Springer Science+Business Media New York

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Zhang, Z., Quan, T., Li, P., Zhang, Y., Sugiura, N., Maekawa, T. (2004). Study on Methane Fermentation and Production of Vitamin B12 from Alcohol Waste Slurry. In: Finkelstein, M., McMillan, J.D., Davison, B.H., Evans, B. (eds) Proceedings of the Twenty-Fifth Symposium on Biotechnology for Fuels and Chemicals Held May 4–7, 2003, in Breckenridge, CO. Biotechnology for Fuels and Chemicals. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-837-3_84

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  • DOI: https://doi.org/10.1007/978-1-59259-837-3_84

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4684-9873-8

  • Online ISBN: 978-1-59259-837-3

  • eBook Packages: Springer Book Archive

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