Abstract
Protein sequence data is now being deduced from cDNA sequences at rates which exceed the capacity of protein chemists to analyze post-translational processing events. Proteins may undergo a large number of post-translational modifications including glycosylation, phosphorylation, proteolytic processing, and disulfide bond formation, among many others (1). At present it is not possible to predict, with accuracy, which post-translational modifications of a particular protein sequence might occur. Principal factors determining post-translational events include primary structure, but higher orders of structure also appear to be important (2). Tissue-specific expression of the processing enzymes is also important (3–5). If the ultimate structure of a gene product is to be determined, it is necessary to purify the proteins or peptides in question and to determine their structures directly.
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References
Wold, F. (1981) Ann. Rev. Biochem. 50, 783–814.
Oroszlan, S. and Copeland, T. D. (1985) Curr. Topics Microbiol. Immunol. 115, 221–233.
Marshak, D., Yamada, T. (1984) Invest. Ophthal. Vis. Sci. 25, 112–115.
Pradayrol, L., Jornvall, H., Mutt, V., and Ribet, A. (1980) FEBS Letters 190, 55–58.
Marshak, D. W., Reeve, J. R., Shively, J. E., Hawke, D., Takami, M. S., and Yamada, T. (1983) J. Neurochem. 41, 601–606.
Hobart, P., Crawford, R., Shen, L.-P., Pictet, R., and Rutter, W. J. (1980) Nature 288, 137–141.
Goodman, R. H., Jacobs, J. W., Chin, W. W., Lund, P. K., Dee, P. C., and Habener, J. F. (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 5869–5873.
Spiess, J. and Noe, B. D. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 277–281.
Andrews, P. C., Hawke, D., Shively, J. E., and Dixon, J. E. (1984) J. Biol. Chem. 259, 5021–5024.
Witten, J. L., Schaffer, M. H., O’Shea, M., Cook, J. C., Hemling, M. E., and Rinehart, K. L. (1984) Biochem. Biophys. Res. Commun. 124, 350–358.
Yazdanparast, R., Andrews, P. C., Smith, D. L., and Dixon, J. E. (1986) Anal. Biochem. 153, 348–353.
Biemann, K. (1982) Int. J. Mass Spectrom. Ion Phys. 45, 183–194.
Pickering, L., Pang, H., Biemann, K., Munro, H., and Schimmel, P. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 2310–2314.
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© 1987 Springer Science+Business Media New York
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Dixon, J.E., Yazdanparast, R., Smith, D., Andrews, P.C. (1987). Identification of Posttranslational Modifications in Neuropeptides. In: Walsh, K.A. (eds) Methods in Protein Sequence Analysis · 1986. Experimental Biology and Medicine, vol 14. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-480-1_41
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DOI: https://doi.org/10.1007/978-1-59259-480-1_41
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4757-5826-9
Online ISBN: 978-1-59259-480-1
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