Abstract
The examination of hard/mineralized tissue has always been regarded as a challenge in the preparation of sections for histomorphopathological study in both clinical and research applications. The time required to decalcify tissue is a recognized extension of the turn-around time in processing, and as a result, a delay in analysis and report generation. Technically, the need for modified embedding (e.g., double embedding such as celloidin or methylsalicylate and paraffin wax) and the use of heavy-duty microtomes such as sledge) adds to the challenge of processing. The need for decalcification complicates the application of phenotypic methods of cell characterization. It is believed that decalcification methods such as acids or kelating agents damage antigenic components of cells and tissue. This would therefore preclude the use of immunohistochemistry or in situ hybridization in the assessment of disease processes in bone.
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Fornasier, V., Ho, C.L. (2003). Radiological Examination of Calcified Tissues with Emphasis on Bone. In: An, Y.H., Martin, K.L. (eds) Handbook of Histology Methods for Bone and Cartilage. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-417-7_37
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DOI: https://doi.org/10.1007/978-1-59259-417-7_37
Publisher Name: Humana Press, Totowa, NJ
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