Abstract
Plant-based expression systems for the production of recombinant proteins have been the subject of numerous studies in recent years. It is now clear that plant-based systems offer a number of substantial advantages over conventional fermentation and cell-culture systems, including the exclusion of mammalian pathogens or bacterial endotoxins, improved scale-up economics, and reduced cost of goods. Plants have also proven to be capable of the assembly of complex multimeric proteins such as secretory IgAs, which are difficult to produce in other systems. However, even among plant systems, there are numerous configurations and modalities for the expression of a particular protein. For example, the actual choice of host species may be affected by a number of considerations, including biochemistry, extraction, and downstream processing or regulatory parameters. Similarly, within a plant, the deposition of the protein in certain tissues, organs, or even subcellular compartments may have a profound effect on the efficiency and economics of production. Although it has proven possible to express recombinant proteins in vegetative cells such as leaves, tubers and roots, there are some singular advantages to the use of seeds as the site of recombinant protein deposition.
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Moloney, M.M. (2003). Oleosin Partitioning Technology for Production of Recombinant Proteins in Oil Seeds. In: Vinci, V.A., Parekh, S.R. (eds) Handbook of Industrial Cell Culture. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-346-0_12
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DOI: https://doi.org/10.1007/978-1-59259-346-0_12
Publisher Name: Humana Press, Totowa, NJ
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