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Microbial DNA and Host Immunity pp 219–226Cite as

Immunostimulatory Sequences in Plasmid Vectors

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Abstract

Immunization with plasmid DNA (pDNA) results in potent humoral and cellular immune responses that are specific to the expressed antigen. Intradermal injection of plasmid DNA (pDNA) in normal saline induces T helper cell differentiation to the T helper 1 type (Th1). This response is characterized by high levels of interferon (IFNy) and the production of Isotype G2a antibodies, as well as the activation of strong major histocompatibility complex (MHC) class I-restricted cytotoxic T-lymphocyte responses (1–5). Additionally, immunization with plasmid DNA can dictate the dominant phenotype of the overall response. Not only can an antigen-specific Th2 response be switched to an antigen-specific Th 1 response by plasmid DNA immunization, but the Th 1 response induced by pDNA immunization also prevails over a later attempt to induce a Th2 response by protein in alum immunization (2). This bias in the T-cell cytokine profile and the potent response relative to the quantitatively small amount of protein produced by the pDNA injection suggested that the DNA was acting as an adjuvant to enhance the response.

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Authors
  1. Christina C. N. Wu
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  2. Chih Min Tang
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  3. Brian Crain
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  4. Maripat Corr
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Editors and Affiliations

  1. University of California, San Diego, La Jolla, CA, USA

    Eyal Raz MD

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© 2002 Humana Press Inc., Totowa, NJ

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Wu, C.C.N., Tang, C.M., Crain, B., Corr, M. (2002). Immunostimulatory Sequences in Plasmid Vectors. In: Raz, E. (eds) Microbial DNA and Host Immunity. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-305-7_17

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  • DOI: https://doi.org/10.1007/978-1-59259-305-7_17

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4684-9728-1

  • Online ISBN: 978-1-59259-305-7

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