Abstract
Electron microscope autoradiography (radioautography) is a technique that involves administration of [3H]-labeled compounds to cells in culture, excised tissues, or whole plants followed by processing of plant material for conventional transmission electron microscopy (TEM) and subsequent application of fine-grain photographic emulsions to thin sections mounted on copper grids (1–9). Tritiated-labeled compounds are employed because the emitted [ß-particle resulting from [3H]-decay (t 1/2= 12.3 yr) is weak and possesses a short pathlength. This is in contrast to the energies and [β-particles resulting from decay of other radionuclides commonly used in life science research (see Chapter 3).
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Dashek, W.V. (2000). Methods in Electron Microscope Autoradiography. In: Dashek, W.V. (eds) Methods in Plant Electron Microscopy and Cytochemistry. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-232-6_17
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DOI: https://doi.org/10.1007/978-1-59259-232-6_17
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61737-199-8
Online ISBN: 978-1-59259-232-6
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