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Handbook of Bacterial Adhesion pp 133–169Cite as

Laboratory Culture and Analysis of Microbial Biofilms

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Abstract

Microbial biofilms develop when bacteria adhere to a substratum and grow inside a secreted extracellular matrix. They can be defined as “matrix-embedded microbial populations adherent to each other and/or to surfaces of interfaces”.31 This is the growth mode for most bacteria. Biofilms are important in human health and disease; for example, the body’s normal flora resists pathogen invasion but can itself turn pathogenic. Biofilm infections are a major problem, especially of prosthetic devices, as 1 to 3% of all orthopedic implant patients experience severe infection following surgery as the probable result of biofilm formation.2 Biofilm formation within a tube can increase frictional resistance over 200%.23 Antibacterial agents, antibiotics, phagocytic white blood cells, and other biocides are much less effective against the bacteria within a biofilm than against planktonic bacteria.52

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Authors and Affiliations

  1. Dental Research Group, Department of Pathology and Molecular Medicine Wellington School of Medicine, University of Otago, Wellington, New Zealand

    Chris H. Sissons & Lisa Wong

  2. Department of Orthopaedic Surgery, Medical University of South Carolina, Charleston, SC, USA

    Yuehuei H. An

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  1. Chris H. Sissons
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  2. Lisa Wong
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  3. Yuehuei H. An
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Editors and Affiliations

  1. Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Medical University of South Carlina, Charleston, SC, USA

    Yuehuei H. An MD (Associate Professor) (Associate Professor)

  2. Department of Orthopaedic, Medical University of South Carlina, Charleston, SC, USA

    Richard J. Friedman MD, FRSC(C) (Professor) (Professor)

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Sissons, C.H., Wong, L., An, Y.H. (2000). Laboratory Culture and Analysis of Microbial Biofilms. In: An, Y.H., Friedman, R.J. (eds) Handbook of Bacterial Adhesion. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-224-1_9

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  • DOI: https://doi.org/10.1007/978-1-59259-224-1_9

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