Fluorescent In Situ Hybridization

  • Susan Sheldon


Fluorescence in situ hybridization (FISH) arose from a marriage of classical DNA hybridization in solution to modern molecular biologic techniques, most notably the use of restriction endonucleases and, later, the polymerase chain reaction (PCR). The former has made identification of both genes and relevant interspersed sequences possible, whereas without the latter, many widely used probes would not be available. FISH allows one to localize a specific DNA sequence to a specific chromosome, region of a chromosome, or cell type. Generally, the morphology of the chromosome, cell, or tissue of interest is preserved to permit unambiguous identification of the target (1–3). Consequently, the technique has been widely used in a variety of venues. Gene mapping (4), chromosome identification (5), demonstration of gene amplification in some solid tumors (6,7), identification of chimeric populations, minimal residual disease, and tumor cells admixed in normal tissue (8,9) are just a few examples. The advantages of modern FISH techniques include:
  1. 1.

    Simple, straightforward, standardized techniques

  2. 2.

    Wide assortment of probes available commercially

  3. 3.

    Double labeling and use of multiple probes on a single cell

  4. 4.

    Rapid turnaround times (anywhere from 4 h to a maximum of 48 h).



Sister Chromatid Cover Slip Painting Probe Kallmann Syndrome Rubber Cement 
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© Springer Science+Business Media New York 2001

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  • Susan Sheldon

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