Application of Immunohistochemistry in the Diagnosis of Lymphoid Lesions

  • Paul J. Kurtin


The value of phenotypic studies to aid in the diagnosis and classification of reactive and neoplastic disorders of lymphocytes and myeloid cells has been established. In general, immunophenotyping has five main goals. First, it can be used to distinguish reactive from neoplastic lymphoid proliferations in lymph nodes and in extranodal sites. Second, it can help to determine that a poorly differentiated malignant neoplasm is a lymphoma. Third, once a neoplasm has been determined to be a lymphoma or leukemia, the marker studies can establish the lineage: B-cell, T-cell, NK cell, myeloid, or histiocytic. These distinctions are clinically important and form the basis for lymphoma classification by the World Health Organization classification of neoplastic diseases of lymphoid tissues (1). Fourth, phenotypic data can be used to help classify lymphomas because some lymphomas express a characteristic constellation of antigens. Finally, phenotypic data can be used to help distinguish Hodgkin’s disease from non-Hodgkin’s lymphomas. Because many different phenotypic modalities are available (flow cytometry, paraffin section immunohistochemistry, frozen section immunohistochemistry, molecular genetics studies) there is often confusion about the best modality to employ to solve specific differential diagnostic problems. The techniques are complementary, and none is perfect for every application. In this chapter, the phenotypic characterization of malignant lymphomas and leukemias by immunohistochemistry is discussed.


Paraffin Section Follicular Lymphoma Mantle Cell Lymphoma Histiocytic Sarcoma Splenic Marginal Zone Lymphoma 
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© Springer Science+Business Media New York 2001

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  • Paul J. Kurtin

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