Immunohistochemistry: Theory and Practice

  • Patrick C. Roche
  • Eric D. Hsi


In the daily practice of surgical pathology, immunohistochemistry (IHC) is essential for the diagnosis and classification of neoplasms. The technique is no longer limited to large university-based or reference laboratories and has become widespread in community hospital 1poratories.. The propagation of commercially available, automated immunostainers has played a major role in increasing the capability to perform IHC and has enabled smaller laboratories to produce quality immunostains without prior technical expertise and without significant increases in personnel. The availability of a large selection of primary antibodies that can be used on formalin-fixed, paraffinembedded tissue sections has also contributed to increased use and sensitivity of the method.


Antigen Retrieval Automate Immunostainers Endogenous Biotin Antigen Retrieval Method Base Mold 
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© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Patrick C. Roche
  • Eric D. Hsi

There are no affiliations available

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