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Peptide Sequence Analysis by Tandem Mass Spectrometry

  • Daniel C. Liebler
Chapter

Abstract

In the last chapter, we considered protein identification with an m/z measurement of the tryptic peptide VGAHAGEYGAEALER from human hemoglobin alpha. When we searched a human/mouse protein sequence database, we found two “perfect” hits for this peptide, both of which had m/z 1529.7384 for the [M+H]+ ion. Both corresponded to hemoglobin alpha peptides that are highly conserved between mice and men:

The human: VGAHAGEYGAEALER

The mouse: IGGHGAEYGAEALER

Keywords

Peptide Amide Alpha Carbon Hemoglobin Alpha Proline Peptide Bond Amino Acid Residue Masse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Suggested Reading

  1. Roepstorff, P. and Fohlman, J. (1984) Proposal for a common nomenclature for sequence ions in mass spectra of peptides. Biomed. Mass Spectrom. 11, 601–601.PubMedCrossRefGoogle Scholar
  2. Yates, J. R. (1998) Mass spectrometry and the age of the proteome. J. Mass. Spectrom. 33, 1–19.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Daniel C. Liebler
    • 1
  1. 1.College of PharmacyThe University of ArizonaTucsonUSA

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