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Mass Spectrometers for Protein and Peptide Analysis

  • Daniel C. Liebler
Chapter

Abstract

Two different types of instruments are used for most proteomics MS work: the MALDI-TOF instruments and the ESI-tandem MS instruments. The two types operate in entirely different ways and generate different, but complementary information. Indeed, the best-equipped proteomics laboratories have both types of instruments available. This chapter describes how each of these instruments works and what types of data they produce, and compares them on the basis of their advantages and limitations. Before we get to the instruments themselves, let’s take a look at the basics of MS instrumentation.

Keywords

Mass Analyzer Quadrupole Mass Analyzer Triple Quad MALDI Source Radiofrequency Voltage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Suggested Reading

  1. Jonscher, K. R. and Yates, J. R. (1997) The quadrupole ion trap mass spectrometer: a small solution to a big challenge. Anal. Biochem. 244,1-15.Google Scholar
  2. Siuzdak, G. (1996) Mass Spectrometry for Biotechnology. Academic Press, San Diego.Google Scholar
  3. Stahl, D. C., Swiderek, K. M., Davis, M. T., and Lee, T. D. (1995) Data-controlled automation of liquid chromatography/tandem mass spectrometry analysis of peptide mixtures. J. Am. Soc. Mass Spectrom. 7, 532–540.CrossRefGoogle Scholar
  4. Yates, J. R. (1998) Mass spectrometry and the age of the proteome. J. Mass. Spectrom. 33, 1–19.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Daniel C. Liebler
    • 1
  1. 1.College of PharmacyThe University of ArizonaTucsonUSA

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