Mining Proteomes

  • Daniel C. Liebler


The purpose of proteome mining is to identify as many of the components of the proteome as possible. In our previous discussions, we have considered “the proteome” as if it were a complete protein complement of all of the organism’s genes. However, this “master” proteome is something of an abstraction. No cell in any organism contains all proteins encoded by its genes all together at one time. Even in yeast, about a third of the yeast genome is not expressed at any given time.


Retinal Pigment Epithelium Peptide Mixture Yeast Proteome Peak Parking Strong Cation Exchange Column 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Suggested Reading

  1. Davis, M. T., Beierle, J., Bures, E. T., McGinley, M. D., Mort, J., Robinson, J. H., Spahr, C. S., Yu, W., Luethy, R., and Patterson, S. D. (2001) Automated LCLC-MS-MS platform using binary ion-exchange and gradient reversed-phase chromatography for improved proteomic analyses. J. Chromatogr. B. Blamed. Sci. Appl. 752, 281–291.CrossRefGoogle Scholar
  2. Gygi, S. P., Corthals, G. L., Zhang, Y., Rochon, Y., and Aebersold, R. (2000) Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology. Proc. Natl. Acad. Sci. USA 97, 9390–9395.PubMedCrossRefGoogle Scholar
  3. Simone, N. L., Paweletz, C. P., Charboneau, L., Petricoin, E. F., and Liotta, L. A. (2000) Laser capture microdissection: beyond functional genomics to proteomics. Mol. Diagn. 5, 301–307.PubMedGoogle Scholar
  4. Washburn, M. P., Wolters, D., and Yates, J. R. (2001) Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat. Biotechnol. 19, 242–247PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Daniel C. Liebler
    • 1
  1. 1.College of PharmacyThe University of ArizonaTucsonUSA

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