Serum and Urine Paraprotein Capillary Electrophoresis

  • Carl R. Jolliff
Part of the Pathology and Laboratory Medicine book series (PLM)


Most requests for serum and urine electrophoresis occur because of the reversal of albumin/globulin ratios in the general chemistry profile or the presence of protein in the urine. The suspicion of possible myelomatosis by the physician also alerts him/her to rule out the presence of a monoclonal gammopathy in the serum or light chain in the urine. Because of the increased sensitivity afforded by the newer electrophoresis systems, paraproteins are found more often. Their appearance in a number of conditions exhibiting a B-cell lymphoproliferative response has renewed the interest of oncologists, hematologists, nephrologists, rheumatologists, internists, and neurologists.


Capillary Electrophoresis Capillary Zone Electrophoresis Monoclonal Gammopathy Free Light Chain Sepharose Bead 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Kyle, R. A. (1993) Benign monoclonal gammopathy: after 20–35 years of follow up. Mayo Clin. Proc. 68, 26–36.Google Scholar
  2. 2.
    Chang, C.-Y., Fritsche, H. A., Classman, A. B., Mc Clure, K. C., and Liu, F. J. (1997) Underestimation of monoclonal proteins by agarose gel serum protein electrophoresis. Ann. Clin. Lab. Sci. 27, 123–129.Google Scholar
  3. 3.
    Jolliff, C. R. and Blessum, C. R. (1997) Comparison of serum protein electrophoresis by agarose gel and capillary zone electrophoresis in a clinical setting. Electrophoresis 18, 1781–1784.CrossRefPubMedGoogle Scholar
  4. 4.
    Katzmann, J. A., Clark, R., Sanders, E., Landers, J. P., and Kyle, R. A. (1998) Prospective study of serum protein capillary electrophoresis immunotyping of monoclonal proteins by immuno-sutraction. Amer. J. Clin. Path. 110, 503–509.Google Scholar
  5. 5.
    Henskens, Y., DeWinter, J., Pekelharing, M., and Ponjee, G. (1998) Detection and identification monoclonal gammopathies by capillary electrophoresis. Clin. Chem. 44, 1184–1190.Google Scholar
  6. 6.
    Bossuyt, X., Bogaerts, G., Shciettekatte, G., and Blanckaert, N. (1998) Detection and classification of paraproteins by capillary immunofixacion/subtraction. Clin. Chem. 44, 760–769.Google Scholar
  7. 7.
    Clark, R., Katzmann, J.A., Wiegert, E., Namyst-Goldberg, C. S., Oda, R. P., Kyle, R. A., and Landers, J. P. (1996) Rapid capillary electrophoretic analysis of human serum proteins: comparison with high throughput agarose gel electrophoresis. J.Chromarogr. A 744, 205–213.Google Scholar
  8. 8.
    Jenkins, M. A. and Guerin, M. D. (1995) Quantification of serum proteins using capillary electrophoresis. Ann. Clin. Biochem. 32, 493–497.Google Scholar
  9. 9.
    Jenkins, M. A., Kulinskaya, E., Martin, H. D., and Guerin, M. D. (1995) Evaluation of serum protein separation by capillary electrophoresis: prospective analysis of 1000 specimens. J. Chromatogr. B 672, 241–251.CrossRefGoogle Scholar
  10. 10.
    Doleman, C. J. A., Siebelder, C. W. M., and Penders, T. J. (1997) Routine serum protein analysis using capillary electrophoresis. Eur. J. Clin. Chem. Clin. Biochem. 35, 393–394.Google Scholar
  11. 11.
    Aguzzi, F. and Poggi, N. (1997) Immunosubtraction electrophoresis. A single method for identifying specific proteins producing the cellulose acetate electropherogram. Ann. Clin. Biochem. 32, 493–497.Google Scholar
  12. 12.
    Klein, G. L. and Jolliff, C. R. (1994) Capillary Electrophoresis for the Routine Clinical Laboratory, Landers, J. P., ed. CRC, Boca Raton FL.Google Scholar
  13. 13.
    Jellum, E., Dollekamp, H., Brunsvig, A., and Gislefoss, R. (1997) Diagnostic applications of chromatography and capillary electrophoresis. J. Chromatogr. B 689, 155–164.CrossRefGoogle Scholar
  14. 14.
    Jenkins, M. A., Leary, T. D., and Guerin, M. D. (1994) Identification and quantitation of human urine proteins by capillary electrophoresis. J. Chromatogr. B 662, 108–112.CrossRefGoogle Scholar
  15. 15.
    Jenkins, M. A. and Guerin, M. D. (1996) Optimization of serum protein separation by capillary electrophoresis (Letter). Clin. Chem. 42, 1886.Google Scholar
  16. 16.
    Blessum, C. R. (1998) Personal communication.Google Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Carl R. Jolliff

There are no affiliations available

Personalised recommendations