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DNA Fingerprinting of Mycobacterium TB: A Rich Source of Fundamental and Daily Applicable Knowledge

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Handbook of Global Tuberculosis Control

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Abstract

This chapter describes the applications of DNA fingerprinting techniques in the molecular epidemiology of TB and the phylogenetic studies on the Mycobacterium tuberculosis complex. The most widely used techniques in the 1990s were IS 6110 restriction fragment length polymorphism (RFLP) and spoligotyping. Currently, the 24 locus Variable Number of Tandem Repeats (VNTP) method has become the international gold standard used for DNA fingerprinting.

These techniques are used to trace the contacts of recently infected individuals to determine the initial source of the TB infection. They are also used to determine if recent cases are the result of reactivation of latent infection, the result of new infection or due to cross-contamination by medical instruments or diagnostic mishaps. Typing techniques also can determine which gene mutations in M. tuberculosis can lead to resistance. Phylogenetic studies can shed light on the evolutionary development of M. tuberculosis.

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Authors and Affiliations

  1. National Tuberculosis Reference Laboratory, Laboratory for Infectious Diseases and Perinatal Screening (LIS), Centre for Infectious Disease Control (CIB), National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands

    Jessica L. de Beer & Dick van Soolingen

  2. Radboud University Nijmegen Medical Centre, location Dekkerswald and Medical Microbiology, Nijmegen, The Netherlands

    Dick van Soolingen

Authors
  1. Jessica L. de Beer
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  2. Dick van Soolingen
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Correspondence to Jessica L. de Beer .

Editor information

Editors and Affiliations

  1. Haikou VTI Biological Institute, Boston, Massachusetts, USA

    Yichen Lu

  2. Chinese Nat’l Ctr Disease Control and Prevention, Nat’l Center for TB Control & Prevention Chinese Nat’l Ctr Disease Control and Prevention, Beijing, China

    Lixia Wang

  3. National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China

    Hongjin Duanmu

  4. Brookline, Massachusetts, USA

    Chris Chanyasulkit

  5. Vaccine Technologies, Inc., Wellesley, Massachusetts, USA

    Amie J. Strong

  6. National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China

    Hui Zhang

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de Beer, J.L., van Soolingen, D. (2017). DNA Fingerprinting of Mycobacterium TB: A Rich Source of Fundamental and Daily Applicable Knowledge. In: Lu, Y., Wang, L., Duanmu, H., Chanyasulkit, C., Strong, A., Zhang, H. (eds) Handbook of Global Tuberculosis Control. Springer, Boston, MA. https://doi.org/10.1007/978-1-4939-6667-7_30

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  • DOI: https://doi.org/10.1007/978-1-4939-6667-7_30

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