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Applications of Mass Spectrometry in Drug Development Science

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Analytical Techniques in the Pharmaceutical Sciences

Part of the book series: Advances in Delivery Science and Technology ((ADST))

Abstract

Mass spectrometry (MS) offers the capability to identify, characterize and quantify a target molecule in a complex sample matrix and has developed into a premier analytical tool in drug development science. Through specific MS-based workflows including customized sample preparation, coupling to liquid chromatography and different ionization principles, both qualitative and quantitative analysis of small and large drug compounds can be achieved at an unprecedented sensitivity.

Here, we review the basic principles of MS and tandem MS, including ionization, mass analysis and detection, as well as fragmentation techniques and coupling of MS to chromatographic separation. As the structural integrity of protein drugs during purification, formulation and delivery is of critical importance to ensure drug efficacy and safety, an overview over current approaches for primary and higher-order structure analysis of proteins by mass spectrometry will be given as well as related workflows for quantitative MS analysis. Established “top-down” and “bottom-up” protein analyses with MS will be recapitulated, and the use of emerging technologies such as hydrogen/deuterium exchange mass spectrometry (HDX-MS) for higher-order protein structure analysis will be discussed.

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Authors and Affiliations

  1. Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Ulrike Leurs, Ulrik H. Mistarz & Kasper D. Rand

Authors
  1. Ulrike Leurs
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  2. Ulrik H. Mistarz
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  3. Kasper D. Rand
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Corresponding author

Correspondence to Kasper D. Rand .

Editor information

Editors and Affiliations

  1. Bioneer: FARMA Department of Pharmacy, University of Copenhagen, Copenhagen Ø, Denmark

    Anette Müllertz

  2. Strathclyde Institute of Pharmacy and Biomedical Sciences, Glasgow, Seychelles

    Yvonne Perrie

  3. Department of Pharmacy, University of Copenhagen, Copenhagen Ø, Denmark

    Thomas Rades

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Leurs, U., Mistarz, U.H., Rand, K.D. (2016). Applications of Mass Spectrometry in Drug Development Science. In: Müllertz, A., Perrie, Y., Rades, T. (eds) Analytical Techniques in the Pharmaceutical Sciences. Advances in Delivery Science and Technology. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-4029-5_7

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