Particle Size Analysis of Micro and Nanoparticles
Micro-and nanosized particles are extensively used in drug formulation, delivery and targeting with the size of these particles fundamentally influencing their properties. There are a range of methods for measuring the size of these system. Here, we evaluate and compare the main particle measurement techniques for micro- and nanosized particles, elucidating on the respective measurement principles. Light scattering based particle sizing techniques are described, including the background to dynamic light scattering, laser diffraction and nanoparticle tracking analysis. Pulse sensing particle sizing methods including Coulter Counter and tunable resistive pulse sensing are described. Two case studies provide practical examples of how to interpret particle size analysis data and how to compare between different measurement techniques.
KeywordsDynamic light scattering Laser diffraction Particle sizing Pulse sensing
The authors gratefully acknowledge Sameer Joshi who generated the plots presented in Fig. 21.5 and Robert Vogel (Izon Science) for reviewing the manuscript. This work was part funded by the EPSRC Centre for Innovative Manufacturing in Emergent Macromolecular Therapies and Aston University.
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