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Genome Editing by Aptamer-Guided Gene Targeting (AGT)

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Book cover Genome Editing

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB))

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Abstract

DNA aptamers are sequences of DNA that because of their unique secondary structure are capable of binding to a specific target. Aptamer technology has only recently been applied to gene correction. The effectiveness of using aptamers for gene targeting comes from their versatility, as aptamers can be used in conjunction with currently existing genome modification systems. Here we describe how DNA aptamers can be exploited to increase donor DNA availability, and thus promote the transfer of genetic information from a donor DNA molecule to a desired chromosomal locus. Although still in its infancy compared to other more well-characterized systems, aptamer-guided gene targeting (AGT) offers a new direction to the field of genetic engineering.

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Acknowledgment

We acknowledge support from the Georgia Cancer Coalition Grant R9028, the NIH Grant R21EB9228, and the Georgia Tech Fund for Innovation in Research and Education GT-FIRE-1021763.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USA

    Patrick Ruff Ph.D.

  2. School of Biology, Georgia Institute of Technology, 310 Ferst Drive, Atlanta, GA, 30332, USA

    Francesca Storici Ph.D.

Authors
  1. Patrick Ruff Ph.D.
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  2. Francesca Storici Ph.D.
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Corresponding author

Correspondence to Francesca Storici Ph.D. .

Editor information

Editors and Affiliations

  1. Inst for Cell & Gene Therapy, Universitaetsklinikum Freiburg, Freiburg, Baden-Württemberg, Germany

    Toni Cathomen

  2. University of North Carolina, Chapel Hill, North Carolina, USA

    Matthew Hirsch

  3. Stanford Medical School, Palo Alto, California, USA

    Matthew Porteus

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© 2016 American Society of Gene and Cell Therapy

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Ruff, P., Storici, F. (2016). Genome Editing by Aptamer-Guided Gene Targeting (AGT). In: Cathomen, T., Hirsch, M., Porteus, M. (eds) Genome Editing. Advances in Experimental Medicine and Biology(). Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3509-3_7

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