Abstract
Usually the history of an enzyme is the narrative of the works to isolate and purify it, measure its molecular weight, determine its crystal configuration, measure its activity, and so on, along, say, 2 or 3 years. On the contrary, the history of Na+/K+-ATPase is a tortuous road full of pitfalls, skirmishes with physical chemistry, thermodynamics, and even philosophy. Fortunately it had a happy end, because it was the first known molecule to produce vectorial movement of ions using chemical energy; to cyclically modify its selectivity; to participate in the action potential; to act as a self-adhesion molecule at cell-cell contacts; to act as receptor of the hormone ouabain, whose main physiological role is to modulate cell contacts; and to generate a Na+ gradient that enables co- and counter-transporters to transport net amount of ions, sugars, and amino acids, i.e., to act as secondary pumps. It is sufficient to say that one of its crucial properties, i.e., to be expressed polarizedly at the intercellular membrane of transporting epithelial cells, involves the β-subunit of the pump that happens to be an adhesion molecule which plays a crucial role in that polarization mechanism.
Keywords
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Acknowledgments
We wish to acknowledge the efficient and pleasant assistance of E. el Oso, A. Castillo, E. Méndez, and M. L. Roldan. Our experimental work was supported by the National Research Council of México (CONACYT) and the Research Council of Mexico City (ICyTDF). IL was recipient of a Postdoctoral Research Fellowship from ICyTDF. O Paez was recipient of a Doctoral Fellowship from CONACYT.
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Cereijido, M., Larre, I., Paez, O., Shoshani, L., Ponce, A. (2016). Na+/K+-ATPase Drives Most Asymmetric Transports and Modulates the Phenotype of Epithelial Cells. In: Hamilton, K., Devor, D. (eds) Ion Channels and Transporters of Epithelia in Health and Disease. Physiology in Health and Disease. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3366-2_10
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