Abstract
The presence of hypoxia in solid tumours is correlated with poor treatment outcome. We have developed a 3-D tissue engineered construct to quantitatively monitor oxygen penetration through tumour tissue using the exogenous 2-nitroimidazole bioreductive probe pimonidazole and phosphorescence quenching technologies. Using this in vitro model we were able to examine the influence of the biguanides metformin and phenformin, antimycin A and KCN, on the distribution and kinetics of oxygen delivery as prototypes of modulators of oxygen metabolism.
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References
Gray LH, Conger AD, Ebert M, Hornsey S, Scott OC (1953) Concentration of oxygen dissolved in tissues at the time of irradiation as a factor in radiotherapy. Br J Radiol 26:638–648
Crabtree H, Cramer W (1933) Action of radium on cancer cells. Proc R Soc B 113:238–250
Nordsmark M, Overgaard M, Overgaard J (1996) Pretreatment oxygenation predicts radiation response in advanced squamous cell carcinoma of the head and neck. Radiother Oncol 41:31–39
Hockel M, Schlenger K, Aral B et al (1996) Association between tumor hypoxia and malignant progression in advanced cancer of the uterine cervix. Cancer Res 56:4509–4515
Movsas B, Chapman JD, Horwitz EM et al (1999) Hypoxic regions exist in human prostate carcinoma. Urology 53:11–18
Brizel DM, Scully SP, Harrelson JM et al (1996) Tumor oxygenation predicts for the likelihood of distant metastases in human soft tissue sarcoma. Cancer Res 56:941–943
Hil SA, Collingridge DR, Vojnovic B, Chaplin DJ (1998) Tumour radiosensitization by high-oxygen-content gases: influence of the carbon dioxide content of the inspired gas on PO2, microcirculatory function and radiosensitivity. Int J Radiat Oncol Biol Phys 40:943–951
Bennett M, Feldmeier J, Smee R, Milross C (2008) Hyperbaric oxygenation for tumour sensitization to radiotherapy: a systematic review of randomized controlled trials. Cancer Treat Rev 34:577–591
Coles C, Williams M, Burnet N (1999) Hyperbaric oxygen therapy. Combination with radiotherapy in cancer is of proved benefit but rarely used. BMJ 318:1076–1077
Horinouchi H, Yamamoto H, Komatsu T, Huang Y, Tsuchida E, Kobayashi K (2007) Enhanced radiation response of a solid tumor with the artificial oxygen carrier ‘albumin-heme’. Cancer Sci 99:1274–1278
Tsuchida E, Sou K, Nakagawa A et al (2009) Artificial oxygen carriers, hemoglobin vesicles and albumin–hemes, based on bioconjugate chemistry. Bioconjug Chem 20:1419–1440
Michaels H, Peterson E, Ling C et al (1981) Combined effects of misonidazole radiosensitization and hypoxic cell toxicity in mammalian cells. Radiat Res 88:354–368
Kyle AH, Baker JHE, Minchinton AI (2012) Targeting quiescent tumour cells via oxygen and IGF-I supplementation. Cancer Res 72:801–809
Kyle AH, Huxham LA, Chiam AS, Sim DH, Minchinton AI (2004) Direct assessment of drug penetration into tissue using a novel application of three dimensional cell culture. Cancer Res 64:6304–6309
Klimant I, Wolfbeis O (1995) Oxygen-sensitive luminescent materials based on silicone-soluble Ruthenium Diimine complexes. Anal Chem 67:3160–3166
Sogame Y, Kitamura A, Yabuki M et al (2009) A comparison of uptake of metformin and phenformin mediated by hOCT1 in human hepatocytes. Biopharm Drug Dispos 30:476–484
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Gandolfo, M.J., Kyle, A.H., Minchinton, A.I. (2016). Tissue Discs: A 3D Model for Assessing Modulation of Tissue Oxygenation. In: Elwell, C.E., Leung, T.S., Harrison, D.K. (eds) Oxygen Transport to Tissue XXXVII. Advances in Experimental Medicine and Biology, vol 876. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3023-4_21
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DOI: https://doi.org/10.1007/978-1-4939-3023-4_21
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