Incorporation of Non-Canonical Amino Acids

  • Lilia Leisle
  • Francis Valiyaveetil
  • Ryan A. Mehl
  • Christopher A. Ahern
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 869)

Abstract

In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical production of semisynthetic proteins have been used successfully for ion channel and receptor studies. The level of difficulty for the application of each approach ranges from trivial to technically demanding, yet all have untapped potential in their application to membrane proteins.

Keywords

Nonsense suppression Evolved tRNA synthetase pairs Intein ligation Protein semi-synthesis 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Lilia Leisle
    • 1
  • Francis Valiyaveetil
    • 2
  • Ryan A. Mehl
    • 3
  • Christopher A. Ahern
    • 1
  1. 1.Department of Molecular Physiology and BiophysicsUniversity of IowaIowa CityUSA
  2. 2.Department of Physiology and PharmacologyOregon Health and Sciences UniversityPortlandUSA
  3. 3.Department of Biochemistry and BiophysicsOregon State University CorvallisCorvallisUSA

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