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Gene Targeting in Crop Species with Effective Selection Systems

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Advances in New Technology for Targeted Modification of Plant Genomes

Abstract

Gene targeting refers to the alteration of a specific DNA sequence in an endogenous gene at its original locus in the genome by homologous recombination and provides a powerful tool for both the functional analysis of the gene of interest and the molecular breeding of crop plants. In this chapter, we focus on gene targeting in crop plants with effective selection systems, namely, gene-specific selection and positive–negative selection. So far japonica rice is the only crop species in which the reproducible gene targeting of the endogenous genes in the nuclear genomes has been reported: two genes by gene-specific selection and at least nine genes by positive–negative selection. Gene-specific selection and positive–negative selection are applicable to only a limited number of genes and, in principle, any genes, respectively. We discuss some characteristic features and possible future developments associated with gene targeting of an endogenous gene with positive–negative selection in crop species.

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Abbreviations

BARI:

Border-associated random integration

BIRI:

Border-independent random integration

DSB:

Double-strand break

EGT:

Ectopic gene targeting

GT:

Gene targeting

HR:

Homologous recombination

NHEJ:

Nonhomologous end joining

OSI:

One-sided invasion

TGT:

True gene targeting

Trp:

Tryptophan

ZFN:

Zinc-finger nuclease

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Acknowledgement

We thank Mikio Nakazono and Hirokazu Kobayashi for their encouragement.

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Authors and Affiliations

  1. Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8601, Japan

    Takaki Yamauchi Ph.D.

  2. Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Yada 52-1, Suruga-ku, 422-8526, Japan

    Shigeru Iida Ph.D.

Authors
  1. Takaki Yamauchi Ph.D.
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  2. Shigeru Iida Ph.D.
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Corresponding author

Correspondence to Takaki Yamauchi Ph.D. .

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Editors and Affiliations

  1. Cellectis Plant Sciences, New Brighton, Minnesota, USA

    Feng Zhang

  2. Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe, Germany

    Holger Puchta

  3. Crop Improvement and Genetics Unit, USDA Agricultural Research Service, Western Regional Research Center, Albany, California, USA

    James G. Thomson

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Yamauchi, T., Iida, S. (2015). Gene Targeting in Crop Species with Effective Selection Systems. In: Zhang, F., Puchta, H., Thomson, J. (eds) Advances in New Technology for Targeted Modification of Plant Genomes. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2556-8_6

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