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Genome Mining for Fungal Secondary Metabolic Gene Clusters

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Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Fungi are known for their ability to produce a vast array of valuable chemical compounds, which are utilized in biotechnology and the pharmaceutical industry. In this chapter, we outline recent advances in the development of genomic and bioinformatics resources for the discovery of genes involved in natural product biosynthesis, focusing on the major natural product classes: polyketides, nonribosomal peptides, and terpenes. These groups of compounds are all produced from simple, scaffold molecules that are further modified to create a diverse portfolio of bioactive products. Biosynthetic clusters of genes responsible for the production of these compounds are prolific in fungal systems and typically include key scaffold-producing enzymes and a multitude of modification enzymes. This chapter highlights how the recent explosion in bioinformatics tools and genomic resources can be applied to mining fungal genomes for the novel natural product biosynthetic pathways, providing key examples of well-studied fungal biosynthetic gene clusters.

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Acknowledgements

Research on terpenoid biosynthesis in C.S-D’s laboratory is supported by the National Institute of Health Grant GM080299 (to C.S-D.). G.T.W. and S.E.B. were supported by the predoctoral National Institute of Health traineeship: GM08700 (G.T.W.) and GM008347 (S.E.B.).

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  1. Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 1479 Gortner Ave., 55108, St. Paul, MN, USA

    Grayson T. Wawrzyn, Mark A. Held PhD, Sarah E. Bloch & Prof. Claudia Schmidt-Dannert PhD

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  1. Grayson T. Wawrzyn
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  2. Mark A. Held PhD
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  3. Sarah E. Bloch
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  4. Prof. Claudia Schmidt-Dannert PhD
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Correspondence to Claudia Schmidt-Dannert PhD .

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  1. Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria

    Susanne Zeilinger

  2. Department of Molecular Biology, University of León, León, Spain

    Juan-Francisco Martín

  3. Biomedicine and Biopharma Area (INBIOTEC), Instituto de Biotecnología de León, León, Spain

    Carlos García-Estrada

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Wawrzyn, G., Held, M., Bloch, S., Schmidt-Dannert, C. (2015). Genome Mining for Fungal Secondary Metabolic Gene Clusters. In: Zeilinger, S., Martín, JF., García-Estrada, C. (eds) Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2531-5_4

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