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Light as a Tool for Biologists: Recent Developments

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Abstract

After a brief introduction, this chapter covers optical tweezers and related techniques, uses of lasers for ablation, desorption, ionization, and dissection, fluorescent labeling, Abbe’s diffraction limit to spatial resolution in microscopy, two-photon excitation fluorescence microscopy, stimulated emission depletion (STED), near-field microscopy, quantum dots and their uses in biology, photochemical internalization and caged compounds, photo-gating of membrane channels, and photo-crosslinking and photolabeling.

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References

  • Ando R (2002) An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci U S A 99:12651–12656

    CAS  PubMed Central  PubMed  Google Scholar 

  • Ashkin A (1970) Acceleration and trapping of particles by radiation pressure. Phys Rev Lett 24:156–159

    CAS  Google Scholar 

  • Bacchi A, Carcelli M, Pelizzi C, Pelizzi G, Pelagatti P, Rogolino D, Tegoni M, Viappiani C (2003) Synthesis and spectroscopic and structural characterization of two novel photoactivatable Ca2+ compounds. Inorg Chem 42:5871–5879

    CAS  PubMed  Google Scholar 

  • Bain JD, Glabe CG, Dix TA, Chamberlin AR, Diala ES (1989) Biosynthetic site-specific incorporation of a non-natural amino acid into a polypeptide. J Am Chem Soc 111:8013–8014

    CAS  Google Scholar 

  • Bakalova R, Zhelev Z, Jose R, Nagase T, Ohba H, Ishikawa M, Baba Y (2005) Role of free cadmium and selenium ions in the potential mechanism for the enhancement of photoluminescence of CdSe quantum dots under ultraviolet irradiation. J Nanosci Nanotechnol 5:887–894

    CAS  PubMed  Google Scholar 

  • Bakalova R, Zhelev Z, Aoki I, Ohba H, Imai Y, Kanno I (2006) Silica-shelled single quantum dot micelles as imaging probes with dual or multimodality. Anal Chem 78:5925–5932

    CAS  PubMed  Google Scholar 

  • Banghart M, Borges K, Isacoff E, Trauner D, Kramer RH (2004) Light-activated ion channels for remote control of neuronal firing. Nature Neurosci 12:1381–1386

    Google Scholar 

  • Bayley H (2006) Sequencing single molecules of DNA. Curr Opin Chem Biol 10:628–637

    CAS  PubMed  Google Scholar 

  • Beale H, Hooley R, Smith SJ, Walker RP (1992) Photoaffinity probes for gibberellin-binding proteins. Phytochemistry 31:1459–1464

    CAS  Google Scholar 

  • Berg K, Selbo K, Prasmickaite L, Tjelle TE, Sandvig K, Moan J, Gaudernack G, Fodstad Ø, Kjølsrud S, Anholt H, Rodal GH, Rodal SK, Høgset A (1999) Photochemical internalization: a novel technology for delivery of macromolecules into cytosol. Cancer Res 59:1180–1183

    CAS  PubMed  Google Scholar 

  • Betzig E, Chichester RJ (1992) Near-field optics: microscopy, spectroscopy, and surface modification beyond the diffraction limit. Science 257:189–195

    CAS  PubMed  Google Scholar 

  • Björn LO, Holmgren B (1996). Monitoring and modelling of the radiation climate. Abisko. Ecol. Bull. 45:204–209

    Google Scholar 

  • Blanck SM, Mehl RA (2006) Systematic study of protein interfaces with genetically incorporated photocrosslinking amino acid, p-benzoylphenylalanine. Abstr Papers Am Chem Soc 231, 794

    Google Scholar 

  • Boatman EM, Lisensky GC, Nordell KJ (2005) A safer, easier, faster synthesis for CdSe quantum dot nanocrystals. J Chem Educ 82:1697–1699

    CAS  Google Scholar 

  • Bouldin KK, Menzel ER, Takatsu M, Murdock RH (2000) Diimide-enhanced fingerprint detection with photoluminescent CdS/dendrimer nanocomposites. J Forensic Sci 45:1239–1242

    CAS  PubMed  Google Scholar 

  • Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K (2005) Millisecond time-scale, genetically targeted optical control of neural activity. Nature Neurosci 8:1263–1268

    CAS  PubMed  Google Scholar 

  • Brunner J (1993) New photolabeling and crosslinking methods. Annu Rev Biochem 62:483–514

    CAS  PubMed  Google Scholar 

  • Brus LE (1984) Electron-electron and electron-hole interactions in small semiconductor crystallites: the size dependence of the lowest excited electronic state. J Chem Phys 80:4403–4409

    CAS  Google Scholar 

  • Cademartiri L, Montanari E, Calestani G, Migliori A, Guagliardi A, Ozin GA (2006) Size-dependent extinction coefficients of PbS quantum dots. J Am Chem Soc 128:10337–10346

    CAS  PubMed  Google Scholar 

  • Callaway EM, Katz LC (1993) Photostimulation using caged glutamate reveals functional circuitry in living brain cells. Proc Natl Acad Sci U S A 90:7661–7665

    CAS  PubMed Central  PubMed  Google Scholar 

  • Chalfie M, Tu Y, Euskirchen G, Ward WW, Prashier DC (1994) Green fluorescent protein as a marker for gene-expression. Science 263:802–805

    CAS  PubMed  Google Scholar 

  • Chambers JJ, Banghart MR, Trauner D, Kramer RH (2006) Light-induced depolarization of neurons using a modified shaker K+ channel and a molecular photo- switch. J Neurophysiol 96:2792–2796

    CAS  PubMed  Google Scholar 

  • Chan PM, Yuen T, Ruf F, Gonzalez-Maeso J, Sealfon SC (2005) Method for multiplex cellular detection of mRNAs using quantum dot fluorescent in situ hybridization. Nucleic Acids Res 33:E161

    PubMed Central  PubMed  Google Scholar 

  • Chandra B, Subramaniam R, Mallik S, Srivastava DK (2006) Formulation of photocleavable liposomes and the mechanism of their content release. Org Biomol Chem 4:1730–1740

    CAS  PubMed  Google Scholar 

  • Chandrasekhar, S. (1947) The transfer of radiation in stellar atmospheres. Bull. Amer. Math. Soc. 53, 641-711. Open access from http://projecteuclid.org/download/pdf_1/euclid.bams/1183510860

  • Chen D, Gibson ES, Kennedy MJ (2013) A light-triggered protein secretion system. J Cell Biol 201:631–640

    CAS  PubMed Central  PubMed  Google Scholar 

  • Chin JW, Martin AB, King DS, Wang L, Schultz PG (2002) Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli. Proc Natl Acad Sci U S A 99:11020–11024

    CAS  PubMed Central  PubMed  Google Scholar 

  • Chu MQ, Song X, Cheng D, Liu SP, Zhu J (2006) Preparation of quantum dot-coated magnetic polystyrene nanospheres for cancer cell labelling and separation. Nanotechnology 17:3268–3273

    CAS  Google Scholar 

  • Cotlet M, Goodwin PM, Waldo GS, Werner JH (2006) A comparison of the fluorescence dynamics of single molecules of a green fluorescent protein: one- versus two-photon excitation. Chem Phys Chem 7:250–260

    CAS  PubMed  Google Scholar 

  • Crut A, Géron-Landre B, Bonnet I, Bonneau S, Desbiolles P, Escude C (2005) Detection of single DNA molecules by multicolor quantum-dot end-labeling. Nucleic Acids Res 33:e98

    PubMed Central  PubMed  Google Scholar 

  • Dacke M, Baird E, Byrne M, Scholtz, CH, Warrant EJ (2013) Dung beetles use the milky way for orientation. Curr Biol 23:298–300

    CAS  PubMed  Google Scholar 

  • Danuser G, Waterman-Storer CM (2006) Quantitative fluorescent speckle microscopy of cytoskeleton dynamics. Annu Rev Biophys Biomol Struct 35:361–387

    CAS  PubMed  Google Scholar 

  • Deisseroth K (2011) Optogenetics. Nat Methods 8:26–29

    CAS  PubMed  Google Scholar 

  • Demeshkina NA, Laletina ES, Meschaninova MI, Repkova MN, Ven’yaminova AG, Graifer DM, Karpova GG (2003) The mRNA codon environment at the P and E sites of human ribosomes deduced from photocrosslinking with pUUUGUU derivatives. Mol Biol 37:132–139 [Transl. from Molekulyarnaya Biologiya, Vol. 37, 147–155]

    CAS  Google Scholar 

  • Deng D-W, Yu J-S, Pan Y (2006) Water-soluble CdSe and CdSe/CdS nanocrystals: a greener synthetic route. J Colloid Interface Sci 299:225–232

    CAS  PubMed  Google Scholar 

  • Derfus AM, Chan WCW, Bhatia SN (2004) Probing the cytotoxicity of semiconductor quantum dots. Nano Lett 4:11–18

    CAS  Google Scholar 

  • Donega CD, Hickey SG, Wuister SF, Vanmaekelbergh D, Meijerink AJ (2003) Single-step synthesis to control the photoluminescence quantum yield and size dispersion of CdSe nanocrystals. Phys Chem B 2003(107):489–496

    Google Scholar 

  • Donnert G, Keller K, Medda R, Andrei MA, Rizzoli SO, Lührmann R, Jahn R, Eggeling C, Hell SW (2006) Macromolecular-scale resolution in biological fluorescence microscopy. Proc Natl Acad Sci U S A 103:11440–11445

    CAS  PubMed Central  PubMed  Google Scholar 

  • Dorman CJ (2007) Probing bacterial nucleoid structure with optical tweezers. BioEssays 29:212–216

    CAS  PubMed  Google Scholar 

  • Dormán G, Prestwich GD (1994) Benzophenone photophores in biochemistry. Biochemistry 33:5561–5673

    Google Scholar 

  • Doroudchi MM, Greenberg KP, Liu J, Silka KA, Boyden ES, Lockridge JA, Arman AC, Janani R, Boye SE, Boye SL, Gordon GM, Matteo BC, Sampath AP, Hauswirth WW, Alan Horsager A (2011) Virally delivered channelrhodopsin-2 safely and effectively restores visual function in multiple mouse models of blindness. Molec Ther 19:1220–1229

    CAS  Google Scholar 

  • Du W, Wang Y, Qingming Luo Q, Liu B-F (2006) Optical molecular imaging for systems biology: from molecule to organism. Anal Bioanal Chem 386:444–457

    CAS  PubMed Central  PubMed  Google Scholar 

  • Eisenman LN, Shu H-J, Akk G, Wang C, Manion BD, Kress GJ, Evers AS, Steinbach JH, Covey DF, Zorumski CF, Mennerick S (2007) Anticonvulsant and anaesthetic effects of a fluorescent neurosteroid analog activated by visible light. Nature Neurosci 10:523–530

    CAS  PubMed  Google Scholar 

  • Elowitz MB, Surette MG, Wolf PE, Stock J, Leibler S (1997) Photoactivation turns green fluorescent protein red. Curr Biol 7:809–812

    CAS  PubMed  Google Scholar 

  • Folgering JHA, Kuiper JM, de Vries AH, Engberts JBFN, Poolman B (2004) Lipid-mediated light activation of a mechanosensitive channel of large conductance. Langmuir 20:6985–6987

    CAS  PubMed  Google Scholar 

  • Fricker M, Runions J, Moore I (2006) Quantitative fluorescence microscopy: from art to science. Annu Rev Plant Biol 57:79–107

    CAS  PubMed  Google Scholar 

  • Geyer H, Geyer R, Pingoud V (2004) A novel strategy for the identification of protein–DNA contacts by photocrosslinking and mass spectrometry. Nucleic Acids Res 32:e132

    PubMed Central  PubMed  Google Scholar 

  • Ghosn B, Haselton FR, Gee KR, Monroe WT (2005) Control of DNA hybridization with photocleavable adducts. Photochem Photobiol 81:953–959

    CAS  PubMed  Google Scholar 

  • Grier DG, Roichman Y (2006) Holographic optical trapping. Appl Optics 45:880–887

    Google Scholar 

  • Gronemeyer H, Pongs O (1980) Localization of ecdysterone on polytene chromosomes of Drosophila melanogaster. Proc Natl Acad Sci U S A 77:2108–2112

    CAS  PubMed Central  PubMed  Google Scholar 

  • Habuchi S, Cotlet M, Gensch T, Bednarz T, Haber-Pohlmeier S, Rozenski J, Dirix G, Michiels J, Vanderleyden J, Heberle J, De Schryver FC, Hofkens J (2005) Evidence for the isomerization and decarboxylation in the photoconversion of the red fluorescent protein DsRed. J Am Chem Soc 127:8977–8984

    CAS  PubMed  Google Scholar 

  • Hammer NI, Early KT, Sill K, Odoi MY, Emrick T, Barnes MD (2006) Coverage-mediated suppression of blinking in solid state quantum dot conjugated organic composite nanostructures. J Phys Chem B 2006(110):14167–14171

    Google Scholar 

  • Hardman R (2006) A toxicologic review of quantum dots: toxicity depends on physicochemical and environmental factors. Environ Health Perspect 114:165–172

    PubMed Central  PubMed  Google Scholar 

  • Heim R (1994) Wavelength mutations and posttranslational autooxidation of green fluorescent protein. Proc Natl Acad Sci U S A 91:12501–12504

    CAS  PubMed Central  PubMed  Google Scholar 

  • Hino M, Okazaki Y, Kobayashi T, Hayashi A, Sakamoto K, Yokoyama S (2005) Protein photo-crosslinking in mammalian cells by site-specific incorporation of a photo-active amino acid. Nat Methods 2:201–206

    CAS  PubMed  Google Scholar 

  • Høgset A, Prasmickaite L, Selbob P-K, Hellumb M, Engesæter BØ, Bonsted A, Berg K (2004) Photochemical internalisation in drug and gene delivery. Adv Drug Deliv Rev 56:95–115

    PubMed  Google Scholar 

  • Hornberg C, Weiler EW (1984) High-affinity binding-sites for abscisic acid on the plasmalemma of Vicia faba guard cells. Nature 310:321–324

    CAS  Google Scholar 

  • Hoshino A, Fujioka K, Oku T, Nakamura S, Suga M, Yamaguchi Y, Suzuki K, Yashara M, Yamamoto K (2004) Quantum dots targeted to the assigned organelle in living cells. Micobiol Immunol 48:985–994

    CAS  Google Scholar 

  • Hu FQ, Ran YL, Zhou ZA, Gao MY (2006) Preparation of bioconjugates of CdTe nanocrystals for cancer marker detection. Nanotechnology 17:2972–2977

    CAS  Google Scholar 

  • Huang J, Xudong Wang X, Wang ZL (2006) Controlled replication of butterfly wings for achieving tunable photonic properties. Nano Lett 6:2325–2331

    CAS  PubMed  Google Scholar 

  • Jiang J, Gill BS (2006) Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research. Genome 49:1057–1068

    CAS  PubMed  Google Scholar 

  • Jiang P, Yamauchi K, Yang M, Tsuji K, Xu MX, Maitra A, Bouvet M, Hoffman RM (2006) Tumor cells genetically labeled with GFP in the nucleus and RFP in the cytoplasm for imaging cellular dynamics. Cell Cycle 5:1198–1201

    CAS  PubMed  Google Scholar 

  • Jorgenson JW, Lukacs KD (1981) Zone electrophoresis in open-tubular glass capillaries. Anal Chem 53:1298–1302

    CAS  Google Scholar 

  • Kim MS, Diamond SL (2006) Photocleavage of o-nitrobenzyl ether derivatives for rapid biomedical release applications. Bioorg Med Chem Lett 16:4007–4010

    CAS  PubMed  Google Scholar 

  • Kippeny T, Swafford LA, Rosenthal SJ (2002) Semiconductor nanocrystals: a powerful visual aid for introducing the particle in a box. J Chem Edu 79:1094–1100

    CAS  Google Scholar 

  • Klostranec JM, Chan CW (2006) Quantum dots in biological and biomedical research: recent progress and present challenges. Adv Mater 2006(18):1953–1964

    Google Scholar 

  • Koçer A, Walko M, Meijberg W, Feringa BL (2005) A light-actuated nanovalve derived from a channel protein. Science 309:755–758

    PubMed  Google Scholar 

  • Krieg A, Ruckstuhl T, Seeger S (2006) Towards single-molecule DNA sequencing: assays with low nonspecific adsorption. Anal Biochem 349:181–185

    CAS  PubMed  Google Scholar 

  • Krüger R, Kübler D, Pallissé R, Burkovski A, Lehmann WD (2006) Protein and proteome phosphorylation stoichiometry analysis by element mass spectrometry. Anal Chem 78:1987–1994

    PubMed  Google Scholar 

  • Lagerholm BC, Averett L, Weinreb GE, Jacobson K, Thompson NL (2006) Analysis method for measuring submicroscopic distances with blinking quantum dots. Biophys J 91:3050–3060

    CAS  PubMed Central  PubMed  Google Scholar 

  • Lester HA, Krouse ME, Nass MM, Wassermann NH, Erlanger BF (1980) A covalently bound photoisomerizable agonist. Comparison with reversibly bound agonists at electrophorus electroplaques. J Gen Physiol 75:207–232

    CAS  PubMed Central  PubMed  Google Scholar 

  • Lewis EK, Haaland WC, Nguyen F, Heller DA, Allen MJ, MacGregor RR, Berger CS, Willingham B, Burns LA, Scott GBI, Kittrell C, Johnson BR, Curl RF, Metzker ML (2005) Color-blind fluorescence detection for four-color DNA sequencing. Proc Natl Acad Sci U S A 102:5346–5351

    CAS  PubMed Central  PubMed  Google Scholar 

  • Li X, Gutierrez DV, Hanson MG, Han J, Mark MD, Chiel H, Hegemann P, Landmesser LT, Herlitze S (2005) Fast noninvasive activation and inhibition of neural and network activity by vertebrate rhodopsin and green algae channelrhodopsin. Proc Natl Acad Sci U S A 102:17816–17821

    CAS  PubMed Central  PubMed  Google Scholar 

  • Li ZH, Wang KM, Tan WH, Li J, Fu ZY, Ma CB, Li HM, He XX, Liu JB (2006) Immunofluorescent labeling of cancer cells with quantum dots synthesized in aqueous solution. Anal Biochem 354:169–174

    CAS  PubMed  Google Scholar 

  • Loudon R, Barnett SM (2006) Theory of the radiation pressure on dielectric slabs, prisms and single surfaces. Opt Express 14:11855–11869

    PubMed  Google Scholar 

  • Lukianova-Hleb EY, Volkov AN, Wu X, Lapotko DO (2013) Transient enhancement and spectral narrowing of the photothermal effect of plasmonic nanoparticles under pulsed excitation. Adv Mater 25:772–776

    CAS  PubMed Central  PubMed  Google Scholar 

  • Lukianova-Hleb EY, Campbell KM, Constantinou PE, Braam J, Olson JS, Ware RE, Sullivan DJ Jr, Lapotko DO (2014) Hemozoin-generated vapor nanobubbles for transdermal reagent- and needle-free detection of malaria. Proc Natl Acad Sci U S A 111:900–905

    CAS  PubMed Central  PubMed  Google Scholar 

  • Lukyanov KA, Chudakov DM, Lukyanov S, Verkhusha VV (2005) Photoactivable fluorescent proteins. Nat Rev Mol Cell Biol 6:885–891

    CAS  PubMed  Google Scholar 

  • Marriott G, Roy P, Jacobson K (2003) Preparation and light-directed activation of caged proteins. Meth Enzymol 360:274–288

    CAS  PubMed  Google Scholar 

  • Marshall P, Heudi O, Bains S, Freeman HN, Abou-Shakra F, Reardon, K (2002) The determination of protein phosphorylation on electrophoresis gel blots by laser ablation inductively coupled plasma-mass spectrometry. Analyst 2:459–461

    Google Scholar 

  • McBride J, Treadway J, Feldman LC, Pennycook SJ, Rosenthal SJ (2006) Structural basis for near unity quantum yield core/shell nanostructures. Nano Lett 6:1496–1501

    CAS  PubMed  Google Scholar 

  • Medintz IL, Uyeda HT, Goldman ER, Mattoussi H (2005) Quantum dot bioconjugates for imaging, labelling and sensing. Nat Mater 4:435–446

    CAS  PubMed  Google Scholar 

  • Menzel ER, Savoy SM, Ulvick SJ, Cheng KH, Murdock RH, Sudduth MR (2000) Photoluminescent semiconductor nanocrystals for fingerprint detection. J Forens Sci 45:545–551

    CAS  Google Scholar 

  • Metzker ML (2005) Emerging technologies in DNA sequencing. Genome Res 15:1767–1776

    CAS  PubMed  Google Scholar 

  • Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S (2005) Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307:538–544

    CAS  PubMed Central  PubMed  Google Scholar 

  • Moan J, Berg K (1991) The photodegradation of porphyrins in cells can be used to estimate the lifetime of singlet oxygen. Photochem Photobiol 53:549–553

    CAS  PubMed  Google Scholar 

  • Mori H, Ito K (2006) Different modes of SecY–SecA interactions revealed by site-directed photo-crosslinking in vivo. Proc Natl Acad Sci U S A 103:16159–16164

    CAS  PubMed Central  PubMed  Google Scholar 

  • Natasha G, Tan A, Farhatnia Y, Rajadas J, Hamblin MR, Peng T, Khaw PT, Seifalian AM (2013) Channelrhodopsins: visual regeneration and neural activation by a light switch. New Biotechnol 30:461–474

    Google Scholar 

  • Ndoye A, Dolivet G, Høgset A, Leroux A, Fifre A, Erbacher P, Berg K, Behró J-P, Guillemin F, Merlin J-L (2006) Eradication of p53-mutated head and neck squamous cell carcinoma xenografts using nonviral p53 gene therapy and photochemical internalization. Mol Ther 13:1156–1162

    CAS  PubMed  Google Scholar 

  • Nechyporuk-Zloy V, Stock C, Schillers H, Oberleithner H, Schwab A (2006) Single plasma membrane K+channel detection by using dual-color quantum dot labeling. Am J Physiol Cell Physiol 291:266–269

    Google Scholar 

  • Nelson T, Tausta SL, Gandotra N, Liu T (2006) Laser dissection of plant tissue: what you see is what you get. Annu Rev Plant Biol 57:181–201

    CAS  PubMed  Google Scholar 

  • Nida DL, Rahman MS, Carlson KD, Richards-Kortum R, Follen M (2005) Fluorescent nanocrystals for use in early cervical cancer detection. Gynecol Oncol 99(Suppl 1):S89–S94

    CAS  PubMed  Google Scholar 

  • O’Connell KMS, Rolig AS, Whitesell JD, Tamkun MM (2006) Kv2.1 Potassium channels are retained within dynamic cell surface microdomains that are defined by a perimeter fence. J Neurosci 26:9609–9618

    PubMed  Google Scholar 

  • Packer AM, Botond Roska B, Häusser M (2013) Targeting neurons and photons for optogenetics. Nature Neurosci 16:805–815

    CAS  PubMed  Google Scholar 

  • Paige JS, Nguyen-Duc T, Song W, Jaffrey SR (2012) Fluorescence imaging of cellular metabolites with RNA. Science 335:1194

    CAS  PubMed Central  PubMed  Google Scholar 

  • Park TJ, Park JP, Seo GM, Chai YG, Lee SY (2006) Rapid and accurate detection of Bacillus anthracis spores using peptide-quantum dot conjugates. J Microbiol Biotechnol 16:1713–1719

    CAS  Google Scholar 

  • Paterson L, Papagiakoumou E, Milne G, Garcés-Chávez V, Tatarkova SA, Sibbett W, Gunn-Moore FJ, Bryant PE, Riches AC, Dholakia K (2005) Light-induced cell separation in a tailored optical landscape. Appl Phys Lett 87:123901

    Google Scholar 

  • Peterman EJG, Brasselet S, Moerner WE (1999) The fluorescence dynamics of single molecules of green fluorescent protein. J Phys Chem A 103:10553–10560

    CAS  Google Scholar 

  • Peterson DS (2007) Matrix-free methods for laser desorption/ionization mass spectrometry. Mass Spectrom Rev 26:19–34

    CAS  PubMed  Google Scholar 

  • Petersson EJ, Brandt GS, Zacharias NM, Dougherty DA, Lester HA (2003) Caging proteins through unnatural amino acid mutagenesis. Meth Enzymol 360:258–273

    CAS  PubMed  Google Scholar 

  • Ramanathan A, Huff EJ, Lamers CC, Potamousis KD, Forrest DK, Schwartz DC (2004) An integrative approach for the optical sequencing of single DNA molecules. Anal Biochem 330:227–241

    CAS  PubMed  Google Scholar 

  • Raymond FR, Ho HA, Peytavi R, Bissonnette L, Boissinot M, Picard FJ, Leclerc M, Bergeron MG (2005) Detection of target DNA using fluorescent cationic polymer and peptide nucleic acid probes on solid support. BMC Biotechn 5, Art. No. 10

    Google Scholar 

  • Rosetti R, Nakahara S, Brus LE (1982) Quantum size effects in the redox potentials, resonance Raman spectra, and electronic spectra of CdS crystallites in aqueous solution. J Chem Phys 79:1086–1088

    Google Scholar 

  • Schaltmann K, Pongs O (1982) Identification and characterization of the ecdysterone receptor in Drosophila melanogaster by photoaffinity labeling. Proc Natl Acad Sci U S A 79:6–10

    CAS  PubMed Central  PubMed  Google Scholar 

  • Schroll C, Riemensperger T, Bucher D, Ehmer J, Völler T, Erbguth K, Berber G, Hendel T, Nagel G, Buchner E, Fiala A (2006) Light-induced activation of distinct modulatory neurons triggers appetitive or aversive learning in Drosophila larvae. Curr Biol 16:1741–1747

    CAS  PubMed  Google Scholar 

  • Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J (2005) Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci U S A 102:5926–5931

    CAS  PubMed Central  PubMed  Google Scholar 

  • Shkrob MA, Yanushevich YG, Chudakov DM, Gurskaya NG, Labas YA, Poponov SY, Mudrik NN, Lukyanov S, Lukyanov KA (2005) Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem J 392:649–654

    CAS  PubMed Central  PubMed  Google Scholar 

  • Simpson GJ (2006) The diffraction barrier broken. Nature 440:879–880

    CAS  PubMed  Google Scholar 

  • So M-K, Xu C, Loening AM, Gambhir SS, Rao J (2006) Self-illuminating quantum dot conjugates for in vivo imaging. Nature Biotechnol 24:339–343

    CAS  Google Scholar 

  • Stiller B, Karageirgiev P, Geue T, Morawetz K, Saphiannikova M, Mechau N, Neher D (2004) Optically induced mass transport studied by scanning near-field optical- and atomic force microscopy. Physics Low Dimens Struct 1–2:129–137

    Google Scholar 

  • Swamy N, Addo JK, Ray R (2000) Development of an affinity-driven crosslinker: Isolation of a vitamin D receptor associated factor. Bioorg Med Chem Lett 10:361–364

    CAS  PubMed  Google Scholar 

  • Synge EH (1928) A suggested method for extending microscopic resolution into the ultra-microscopic region. Phil Mag 6:356–362

    CAS  Google Scholar 

  • Todoroki Y, Tanaka T, Kisamori M, Hirai N (2001) 3-Azidoabscissic acid as a photoaffinity reagent for abscisic acid binding proteins. Bioorg Med Chem Lett 11:2381–2384

    CAS  PubMed  Google Scholar 

  • Torimoto T, Murakami S, Sakuraoka M, Iwasaki K, Okazaki K, Shibayama T, Ohtani B (2006) Photochemical fine-tuning of luminescent color of cadmium selenide nanoparticles: fabricating a single-source multicolor luminophore. J Phys Chem 110:13314–13318

    CAS  Google Scholar 

  • Tsien RY (1998) The green fluorescent protein. Annu Rev Biochem 67:509–544

    CAS  PubMed  Google Scholar 

  • Verkhusha VV, Lukyanov KA (2004) The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins. Nature Biotechnol 22:289–296

    CAS  Google Scholar 

  • Viviani VR (1999) Cloning, sequence analysis, and expression of active Phrixothrix railroad-worms luciferases: relationship between bioluminescence spectra and primary structures. Biochemistry 38:8271–8279

    CAS  PubMed  Google Scholar 

  • Wang LV (2009) Multiscale photoacoustic microscopy and computed tomography. Nat Photonics 3:503–509

    CAS  PubMed Central  PubMed  Google Scholar 

  • Wang LV, Hu S (2012) Photoacoustic tomography: In vivo imaging from organelles to organs. Science 335:1458–1462. doi:10.1126/science.1216210

    CAS  PubMed Central  PubMed  Google Scholar 

  • Wang QB, Seo DK (2006) Synthesis of deep-red-emitting CdSe quantum dots and general non-inverse-square behavior of quantum confinement in CdSe quantum dots. Chem Mater 18:5764–5767

    CAS  Google Scholar 

  • Wang L, Brock A, Herberich B, Schultz PG (2001) Expanding the genetic code of Escherichia coli. Science 292:498–500

    CAS  PubMed  Google Scholar 

  • Werner JH, Cai H, Jett JH, Reha-Krantz L, Keller RA, Goodwin PM (2003) Progress towards single-molecule DNA sequencing: a one color demonstration. J Biotechnol 102:1–14

    CAS  PubMed  Google Scholar 

  • Winkler LD, Arceo JF, Hughes WC, DeGraff BA, Augustine BH (2005) Quantum dots: an experiment for physical or materials chemistry. J Chem Educ 82:1700–1702

    CAS  Google Scholar 

  • Wittelsberger A, Thomas BE, Mierke DF, Rosenblatt M (2006) Methionine acts as a “magnet” in photoaffinity crosslinking experiments. FEBS Lett 580:1872–1876

    CAS  PubMed  Google Scholar 

  • Xie J, Schultz PG (2005) Adding amino acids to the genetic repertoire. Curr Opin Chem Biol 9:548–554

    CAS  PubMed  Google Scholar 

  • Xie J, Schultz PG (2006) A chemical toolkit for proteins an expanded genetic code. Nature Rev Mol Cell Biol 7:775–782

    CAS  Google Scholar 

  • Xu WB, Wang YX, Xu RH, Yin DZ (2006) Synthesis, modification and application in biology of quantum dots (in Chinese). J Inorg Mater 21:1031–1037

    CAS  Google Scholar 

  • Yeh H-C, Ho Y-P, Shih I-M, Wang T-H (2006) Homogeneous point mutation detection by quantum dot-mediated two-color fluorescence coincidence analysis. Nucleic Acids Res 34:e35

    PubMed Central  PubMed  Google Scholar 

  • Zhang F, Wang LP, Boyden ES, Deisseroth K (2006) Channelrhodopsin-2 and optical control of excitable cells. Nat Methods 3:785–792

    CAS  PubMed  Google Scholar 

  • Zheng J, Nicovich PR, Dickson RM (2007) Highly fluorescent noble-metal quantum dots. Annu Rev Phys Chem 58:409–431

    CAS  PubMed Central  PubMed  Google Scholar 

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Authors and Affiliations

  1. School of Life Science, South China Normal University, Guangzhou, China

    Lars Olof Björn

  2. Department of Biology, Lund University, Lund, Sweden

    Lars Olof Björn

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  1. School of Life Science, Department of Biology, South China Normal University, Guangzhou, China & Lund University, Lund, Sweden

    Lars Olof Björn

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Björn, L.O. (2015). Light as a Tool for Biologists: Recent Developments. In: Björn, L. (eds) Photobiology. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1468-5_5

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