Sperm Binding to the Zona Pellucida, Hyaluronic Acid Binding Assay, and PICSI



Work derived from the IVF arena has demonstrated that an abnormal sperm–zona pellucida interaction is frequently observed in infertile men. Such finding can be observed in the presence of normal or abnormal “basic” sperm parameters. An impaired sperm–zona pellucida interaction can result in failure of fertilization, thereby, decreasing chances of pregnancy when couples are being subjected to intrauterine insemination (IUI) or conventional IVF therapies. Consequently, sperm–zona pellucida binding assays were developed as a real need to assess sperm functional competence in the “extended” evaluation of the infertility work-up. The sperm Hyaluronic Binding Acid (HBA) assay has been applied to select sperm for ICSI. It has been proposed that the results of the test indicate that the selected spermatozoa have undergone normal spermatogenesis, but more studies are needed to prove its clinical value.


Hyaluronic Acid Zona Pellucida Acrosome Reaction Human Oocyte Intrauterine Insemination 
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© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Obstetrics and Gynecology, The Jones Institute for Reproductive MedicineEastern Virginia Medical SchoolNorfolkUSA

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