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Plastid-Based Expression Strategies

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Genetically Engineered Plants as a Source of Vaccines Against Wide Spread Diseases

Abstract

Since the development of methodologies for achieving genetic modification of chloroplast genomes, termed transplastomic technologies, a straightforward use in the field of plant-based vaccines was initiated. Chloroplast transformation is mediated by homologous recombination that allows for site-specific insertion of foreign DNA into the plastome. This focus offers substantial advantages that include high yields, improved biosafety given by maternal inheritance in most plant species, and multigene expression through polycistrons allowing in theory for the straightforward production of multicomponent vaccines. One limitation of the system consists of the lack of glycosylation pathways, which are of relevance in some cases. State of the art in this area reflects a number of well-characterized vaccination models, although no one has passed clinical evaluations, which contrasts with other nuclear transient expression systems. Transplastomic technologies are envisioned as a relevant tool for developing new convenient vaccines.

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Authors and Affiliations

  1. Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, 78210, San Luis Potosí, SLP, México

    Jorge A. Salazar-González, Elizabeth Monreal-Escalante & Sergio Rosales-Mendoza

  2. Department I—Botany, Faculty of Biology, Ludwig-Maximilians-Universität, Menzinger Str. 67, 80638, München, Germany

    Areli Herrera Díaz & Hans Ulrich Koop

  3. Department of Environmental Science, Institute of Biochemical Plant Pathology, Helmholtz Center Munich, Neuherberg, Germany

    Areli Herrera Díaz

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  1. Jorge A. Salazar-González
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  2. Elizabeth Monreal-Escalante
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  3. Areli Herrera Díaz
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  4. Hans Ulrich Koop
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  5. Sergio Rosales-Mendoza
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Correspondence to Sergio Rosales-Mendoza .

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    Sergio Rosales-Mendoza

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Salazar-González, J., Monreal-Escalante, E., Díaz, A., Koop, H., Rosales-Mendoza, S. (2014). Plastid-Based Expression Strategies. In: Rosales-Mendoza, S. (eds) Genetically Engineered Plants as a Source of Vaccines Against Wide Spread Diseases. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-0850-9_4

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