Rapid Diagnostic Tests for Oxidative Stress Status

Chapter
Part of the Oxidative Stress in Applied Basic Research and Clinical Practice book series (OXISTRESS)

Abstract

Within the field of free radicals in biology and medicine, there is steadily increasing evidence suggesting the general importance of oxidative stress and related inflammation disorders to human health. There is also an increasing need to translate this knowledge into practical applications for the human population. However, many problems currently need to be solved in the development of the oxidative stress profile for clinical use.

In this chapter, the importance of assessing oxidative stress in order to understand the pathophysiology of various disorders in children is described, followed by an introduction of the recent advances in an easy, rapid, specific, and sensitive diagnostic assay of oxidative stress, i.e., point-of-care testing, using the automatic analyzers FRAS4 (Free Radical Analytical System 4; Diacron International srl., Grosseto, Italy) and ICR-001 (Techno Medica Co., Ltd., Yokohama, Japan). The former was designed for successive measurement of the blood levels of reactive oxygen species and the biological antioxidant potential, while the latter was exclusively developed for the assessment of urinary concentrations of 8-oxo-7,8-dihydro-2′-deoxyguanosine, a well-known biomarker for oxidative DNA damage.

Keywords

High Performance Liquid Chromatography Lymphoma Leukemia Creatinine Adduct 

Abbreviations

8-OHdG

8-hydroxy-2′-deoxyguanosine

8-oxodG

8-oxo-7,8-dihydro-2′-deoxyguanosine

BAP

Biological antioxidant potential

d-ROMs

Derivatives of reactive oxygen metabolites

ELISA

Enzyme-linked immunosorbent assay

FRAS4

Free radical analytical system 4

INS

Idiopathic nephrotic syndrome

IVIG

Intravenous immunoglobulin therapy

KD

Kawasaki disease

MELAS

Myopathy encephalopathy, lactic acidosis, and stroke-like episodes

OS

Oxidative stress

OSI

Oxidative stress index

POCT

Point-of-care testing

ROS

Reactive oxygen species

Notes

Acknowledgments

This study was partly funded by grants from the Mami Mizutani Foundation and the Human Resource Chair for Neonatologists funded by Izumi-Ohtsu City, Osaka, Japan and by the Chair for Children with Developmental Disorders funded by Nabari City, Mie, Japan.

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  1. 1.Department of PediatricsKansai Medical UniversityHirakata-shi, OsakaJapan

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