Lactococcus lactis: Recent Developments in Functional Expression of Membrane Proteins

  • Sana Bakari
  • François André
  • Daphné Seigneurin-Berny
  • Marcel Delaforge
  • Norbert Rolland
  • Annie Frelet-BarrandEmail author


Membrane proteins (MPs) play key roles in most important cellular processes ranging from cell-to-cell communication to signaling processes. Despite recent improvements, the expression of functionally folded MPs in sufficient amounts for functional and structural characterization remains a challenge. Indeed, it is still difficult to predict whether a protein can be overproduced in a functional state in some expression system(s), although studies of high-throughput screens have been issued in recent years. Prokaryotic expression systems present several advantages over eukaryotic ones. Among them, Lactococcus lactis (L. lactis) has emerged in the past years as a good alternative expression system to Escherichia coli. The purpose of this chapter is to describe L. lactis and its tightly inducible system, nisin-controlled gene expression (NICE), for the expression of MPs from both prokaryotic and eukaryotic origins. Moreover, we will describe the functional characterization of some MPs produced in L. lactis.


Cystic Fibrosis Transmembrane Conductance Regulator Lactococcus Lactis Expression Yield Chloroplast Envelope Nice System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work has been supported by the Region Ile de France (DIM SeNt, PhD fellowship to SB AAP2010-3-10T6). We thank Edmund Kunji for his kind help and critical reading of the chapter.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Sana Bakari
    • 1
  • François André
    • 1
  • Daphné Seigneurin-Berny
    • 2
  • Marcel Delaforge
    • 1
  • Norbert Rolland
    • 2
  • Annie Frelet-Barrand
    • 1
    Email author
  1. 1.Oxidative Stress and Detoxification Laboratory, UMR-CNRS 8221Institute of Biology and Technology of Saclay, SB2SM, and Centre for Nuclear Studies and Université Paris-SudsGif-sur-YvetteFrance
  2. 2.Cell & Plant Physiology Laboratory, Institute of Sciences Research and Technologies, UMR-CNRS 5168National Institute of Agronomical Research, Centre for Nuclear Studies, Université Grenoble AlpesGrenobleFrance

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