Oxygen Diffusion: An Enzyme-Controlled Variable Parameter

  • Wilhelm Erdmann
  • Stefan Kunke
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 812)


Previous oxygen microelectrode studies have shown that the oxygen diffusion coefficient (DO2) increases during extracellular PO2 decreases, while intracellular PO2 remained unchanged and thus cell function (spike activity of neurons). Oxygen dependency of complex multicellular organisms requires a stable and adequate oxygen supply to the cells, while toxic concentrations have to be avoided. Oxygen brought to the tissue by convection diffuses through the intercellular and cell membranes, which are potential barriers to diffusion. In gerbil brain cortex, PO2 and DO2 were measured by membrane-covered and by bare gold microelectrodes, as were also spike potentials. Moderate respiratory hypoxia was followed by a primary sharp drop of tissue PO2 that recovered to higher values concomitant with an increase of DO2. A drop in intracellular PO2 recovered immediately. Studies on the abdominal ganglion of aplysia californica showed similar results.

Heterogeneity is a feature of both normal oxygen supply to tissue and supply due to a wide range of disturbances in oxygen supply. Oxygen diffusion through membranes is variable thereby ensuring adequate intracellular PO2. Cell-derived glucosamine oxidase seems to regulate the polymerization/depolymerisation ratio of membrane mucopolysaccharides and thus oxygen diffusion.

Variability of oxygen diffusion is a decisive parameter for regulating the supply/demand ratio of oxygen supply to the cell; this occurs in highly developed animals as well as in species of a less sophisticated nature. Autoregulation of oxygen diffusion is as important as the distribution/perfusion ratio of the capillary meshwork and as the oxygen extraction ratio in relation to oxygen consumption of the cell. Oxygen diffusion resistance is the cellular protection against luxury oxygen supply (which can result in toxic oxidative species leading to mutagenesis).


Oxygen diffusion Glucosamine oxidase Enzyme controlled O2 diffusion Mucopolysaccharides Tissue O2 autoregulation 


  1. 1.
    Erdmann W (1992) Origin of oxygen. Adv Exp Med Biol 317:7–17CrossRefPubMedGoogle Scholar
  2. 2.
    Erdmann W, Krell (1976) Measurement of diffusion parameters with noble metal electrodes. Adv Exp Med Biol 75:225–228CrossRefPubMedGoogle Scholar
  3. 3.
    Clark DK, Erdmann W, Halsey JH, Strong E (1978) Oxygen diffusion, conductivity and solubility coefficients in the microarea of the brain. Adv Exp Med Biol 94:697–704CrossRefGoogle Scholar
  4. 4.
    Kunke S, Erdmann W, Metzger H (1972) A new method for simultaneous PO2 and action potential measurement in microareas of tissue. J Appl Physiol 32:436–438CrossRefPubMedGoogle Scholar
  5. 5.
    Erdmann W, Fennema M, van Kesteren R (1992) Factors that determine the oxygen supply of the cell and their possible disruption. Adv Exp Med Biol 317:607–622CrossRefPubMedGoogle Scholar
  6. 6.
    Nemoto EM, Erdmann W, Strong E, Rao GR, Moosy J (1977) Regional brain PO2 after global ischemia in monkeys: evidence for regional differences in critical perfusion pressures. Stroke 8:558–564CrossRefPubMedGoogle Scholar
  7. 7.
    Morawetz R, Strong E, Clark DK, Erdmann W (1978) Effects of ischemia on the diffusion coefficients in the brain cortex. Adv Exp Med Biol 94:629–632CrossRefGoogle Scholar
  8. 8.
    Chen C, Erdmann W, Halsey J (1978) The sensitivity of applysia giant neurons to changes in extracellular and intracellular PO2. Adv Exp Med Biol 94:691–696CrossRefGoogle Scholar
  9. 9.
    Swartz HM (1994) Measurement of intracellular concentrations of oxygen: Experimental results and conceptual implications of an observed gradient between intracellular and extracellular concentrations of oxygen. Adv Exp Med Biol 345:799–806CrossRefPubMedGoogle Scholar
  10. 10.
    Khan N, Shen J, Chang TY, Chang CC, Swartz H et al (2003) Plasma membranes cholesterol: a possible barrier to intracellular oxygen in normal and mutant CHO cells defective cholesterol metabolism. Biochemistry 42:23–29CrossRefPubMedGoogle Scholar
  11. 11.
    Chen K, Ng CE, Zweier JL, Kuppusamy P, Glickson JD, Schwartz HM (1994) Measurement of the intracellular concentrations of oxygen in a cell perfusion system. Magn Reson Med 31:668–672CrossRefPubMedGoogle Scholar
  12. 12.
    de Duve C (1984) A guided tour of the living cell. Scientific American Books, New YorkGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  1. 1.Erasmus University RotterdamCulemborgThe Netherlands

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