Vessel Specific Imaging of Glucose Transfer with Fluorescent Glucose Analogue in Anesthetized Mouse Cortex

  • Rei Murata
  • Yuki Takada
  • Hiroyuki Takuwa
  • Hiroshi Kawaguchi
  • Hiroshi Ito
  • Iwao Kanno
  • Naotomo Tottori
  • Yukio Yamada
  • Yutaka Tomita
  • Yoshiaki Itoh
  • Norihiro Suzuki
  • Katsuya Yamada
  • Kazuto Masamoto
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 812)

Abstract

The present study examined glucose transfer in the cellular scale of mouse brain microvasculature in vivo using two-photon microscopy and fluorescent glucose analogue (2-NBDG). The 2-NBDG was intravenously injected (0.04 mL/min) in the anesthetized Tie2-GFP mice in which the vascular endothelium expressed fluorescent protein. Time-lapse imaging was conducted on the cortical parenchyma, while the time–intensity change of the injected 2-NBDG was analysed in respective vascular compartments (artery, capillary, and vein). We observed that 2-NBDG signal increased monotonically in the vasculature during the period of the injection, and rapidly declined following its cessation. In tissue compartment, however, the signal intensity gradually increased even after cessation of the injection. Spatiotemporal analysis of the 2-NBDG intensity over the cross-sections of the vessels further showed distinct change of the 2-NBDG intensity across the vessel wall (endothelium), which may represents a regulation site of tissue glucose influx.

Keywords

Optical imaging Glucose transporter Vascular endothelial cell 2-NBDG Two-photon microscopy 

Notes

Acknowledgments

This study was partially supported through funding from JSPS KAKENHI (#25750400) (to K.M.), JSPS KEKENHI (#24659578) (to I.K.), and a grant from the Ministry of Health, Labor and Welfare (MHLW), Japan (to I.K.).

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Rei Murata
    • 1
  • Yuki Takada
    • 1
  • Hiroyuki Takuwa
    • 2
  • Hiroshi Kawaguchi
    • 2
  • Hiroshi Ito
    • 2
  • Iwao Kanno
    • 2
  • Naotomo Tottori
    • 1
  • Yukio Yamada
    • 1
  • Yutaka Tomita
    • 3
  • Yoshiaki Itoh
    • 3
  • Norihiro Suzuki
    • 3
  • Katsuya Yamada
    • 4
  • Kazuto Masamoto
    • 5
    • 2
    • 6
  1. 1.Department of Mechanical Engineering and Intelligent SystemThe University of Electro-CommunicationsTokyoJapan
  2. 2.Molecular Imaging CenterNational Institute of Radiological SciencesChibaJapan
  3. 3.Department of NeurologyKeio University School of MedicineTokyoJapan
  4. 4.Department of PhysiologyHirosaki University Graduate School of MedicineHirosakiJapan
  5. 5.Department of Mechanical Engineering and Intelligent System, Graduate School of Informatics and EngineeringThe University of Electro-CommunicationsChofu, TokyoJapan
  6. 6.Brain Science Inspired Life Support Research CenterUniversity of Electro-CommunicationsChofu, TokyoJapan

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