Abstract
The present study examined glucose transfer in the cellular scale of mouse brain microvasculature in vivo using two-photon microscopy and fluorescent glucose analogue (2-NBDG). The 2-NBDG was intravenously injected (0.04 mL/min) in the anesthetized Tie2-GFP mice in which the vascular endothelium expressed fluorescent protein. Time-lapse imaging was conducted on the cortical parenchyma, while the time–intensity change of the injected 2-NBDG was analysed in respective vascular compartments (artery, capillary, and vein). We observed that 2-NBDG signal increased monotonically in the vasculature during the period of the injection, and rapidly declined following its cessation. In tissue compartment, however, the signal intensity gradually increased even after cessation of the injection. Spatiotemporal analysis of the 2-NBDG intensity over the cross-sections of the vessels further showed distinct change of the 2-NBDG intensity across the vessel wall (endothelium), which may represents a regulation site of tissue glucose influx.
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References
Yoshioka K, Takahashi H, Homma T, Saito M, Oh KB, Nemoto Y, Matsuoka H (1996) A novel fluorescent derivative of glucose applicable to the assessment of glucose uptake activity of Escherichia coli. Biochim Biophys Acta 1289:5–9
Yamada K, Saito M, Matsuoka H, Inagaki N (2007) A real-time method of imaging glucose uptake in single, living mammalian cells. Nat Protoc 2:753–762
Yamada K, Nakata M, Horimoto N, Saito M, Matsuoka H, Inagaki N (2000) Measurement of glucose uptake and intracellular calcium concentration in single, living pancreatic beta-cells. J Biol Chem 275:22278–22283
Yoshioka K, Saito M, Oh KB, Nemoto Y, Matsuoka H, Natsume M, Abe H (1996) Intracellular fate of 2-NBDG, a fluorescent probe for glucose uptake activity, in Escherichia coli cells. Biosci Biotechnol Biochem 60:1899–1901
Motoike T, Loughna S, Perens E, Roman BL, Liao W, Chau TC, Richardson CD, Kawate T, Kuno J, Weinstein BM, Stainier DY, Sato TN (2000) Universal GFP reporter for the study of vascular development. Genesis 28:75–81
Itoh Y, Toriumi H, Yamada S, Hoshino H, Suzuki N (2010) Resident endothelial cells surrounding damaged arterial endothelium reendothelialize the lesion. Arterioscler Thromb Vasc Biol 30:1725–1732
Masamoto K, Tomita Y, Toriumi H, Aoki I, Unekawa M, Takuwa H, Itoh Y, Suzuki N, Kanno I (2012) Repeated longitudinal in vivo imaging of neuro-gliovascular unit at the peripheral boundary of ischemia in mouse cerebral cortex. Neuroscience 212:190–200
Tomita Y, Kubis N, Calando Y, Tran Dinh A, Méric P, Seylaz J, Pinard E (2005) Long-term in vivo investigation of mouse cerebral microcirculation by fluorescence confocal microscopy in the area of focal ischemia. J Cereb Blood Flow Metab 25(7):858–867
Acknowledgments
This study was partially supported through funding from JSPS KAKENHI (#25750400) (to K.M.), JSPS KEKENHI (#24659578) (to I.K.), and a grant from the Ministry of Health, Labor and Welfare (MHLW), Japan (to I.K.).
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Murata, R. et al. (2014). Vessel Specific Imaging of Glucose Transfer with Fluorescent Glucose Analogue in Anesthetized Mouse Cortex. In: Swartz, H.M., Harrison, D.K., Bruley, D.F. (eds) Oxygen Transport to Tissue XXXVI. Advances in Experimental Medicine and Biology, vol 812. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-0620-8_32
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DOI: https://doi.org/10.1007/978-1-4939-0620-8_32
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