Automated Image Analysis for Diameters and Branching Points of Cerebral Penetrating Arteries and Veins Captured with Two-Photon Microscopy

  • Takuma Sugashi
  • Kouichi Yoshihara
  • Hiroshi Kawaguchi
  • Hiroyuki Takuwa
  • Hiroshi Ito
  • Iwao Kanno
  • Yukio Yamada
  • Kazuto Masamoto
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 812)

Abstract

The present study was aimed to characterize 3-dimensional (3D) morphology of the cortical microvasculature (e.g., penetrating artery and emerging vein), using two-photon microscopy and automated analysis for their cross-sectional diameters and branching positions in the mouse cortex. We observed that both artery and vein had variable cross-sectional diameters across cortical depths. The mean diameter was similar for both artery (17 ± 5 μm) and vein (15 ± 5 μm), and there were no detectable differences over depths of 50–400 μm. On the other hand, the number of branches was slightly increased up to 400-μm depth for both the artery and vein. The mean number of branches per 0.1 mm vessel length was 1.7 ± 1.2 and 3.8 ± 1.6 for the artery and vein, respectively. This method allows for quantification of the large volume data of microvascular images captured with two-photon microscopy. This will contribute to the morphometric analysis of the cortical microvasculature in functioning brains.

Keywords

Brain microcirculation Blood flow regulation Mouse cortex 3D-image reconstruction Vascular network structure 

Notes

Acknowledgments

This study was partially supported through funding from JSPS KAKENHI (#25750400) (to K.M.), JSPS KEKENHI (#24659578) (to I.K.), and a grant from the Ministry of Health, Labor and Welfare (MHLW), Japan (to I.K.).

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Takuma Sugashi
    • 1
  • Kouichi Yoshihara
    • 1
  • Hiroshi Kawaguchi
    • 2
  • Hiroyuki Takuwa
    • 2
  • Hiroshi Ito
    • 2
  • Iwao Kanno
    • 2
  • Yukio Yamada
    • 1
  • Kazuto Masamoto
    • 1
    • 2
    • 3
  1. 1.Department of Mechanical Engineering and Intelligent System, Graduate School of Informatics and EngineeringThe University of Electro-CommunicationsChofuJapan
  2. 2.Molecular Imaging CenterNational Institute of Radiological SciencesChibaJapan
  3. 3.Brain Science Inspired Life Support Research CenterUniversity of Electro-CommunicationsChofuJapan

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