Repetitive Measurements of Intrarenal Oxygenation In Vivo Using L Band Electron Paramagnetic Resonance

  • Stephanie Franzén
  • Liselotte Pihl
  • Nadeem Khan
  • Fredrik Palm
  • Håkan Gustafsson
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 812)

Abstract

Intrarenal oxygenation is heterogeneous with oxygen levels normally being highest in the superficial cortex and lowest in the inner medulla. Reduced intrarenal oxygenation has been implied in the pathology of several kidney diseases. However, there is currently no method available to repetitively monitor regional renal oxygenation using minimally invasive procedures. We therefore evaluated implantable lithium phthalocyanine (LiPc) probes, which display a close correlation between EPR line width and oxygen availability.

LiPc probes were implanted in the kidney cortex and medulla in the same mouse and EPR spectra were acquired using a L band scanner during inhalation of air (21 % oxygen) or a mixture of air and nitrogen (10 % oxygen). In order to separate the signals from the two probes, a 1 G/cm gradient was applied and the signals were derived from 40 consecutive sweeps. Peak-to-peak comparison of the EPR line was used to convert the signal to an approximate oxygen tension in MATLAB. Kidney cortex as well as medullary oxygenation was stable over the 45 day period (cortex 56 ± 7 mmHg and medulla 43 ± 6 mmHg). However, 10 % oxygen inhalation significantly reduced oxygenation in both cortex (56 ± 6 to 34 ± 2 mmHg n = 15 p < 0.05) and medulla (42 ± 5 to 29 ± 3 mmHg n = 7 p < 0.05).

In conclusion, L band EPR using LiPc probes implanted in discrete intrarenal structures can be used to repetitively monitor regional renal oxygenation. This minimally invasive method is especially well suited for conditions of reduced intrarenal oxygenation since this increases the signal intensity which facilitates the quantification of the EPR signal to absolute oxygenation values.

Keywords

Kidney LiPc L-Band EPR NMRI mice Oxygenation 

Notes

Acknowledgments

We are deeply grateful to Dr. Harold Swartz and the EPR Center, Hanover, New Hampshire, USA for technical assistance.

References

  1. 1.
    Palm F, Cederberg J, Hansell P, Liss P, Carlsson PO (2003) Reactive oxygen species cause diabetes-induced decrease in renal oxygen tension. Diabetologia 46(8):1153–1160CrossRefPubMedGoogle Scholar
  2. 2.
    Wilcox CS, Palm F, Welch WJ (2013) Renal oxygenation and function of the rat kidney: effects of inspired oxygen and preglomerular oxygen shunting. Adv Exp Med Biol 765:329–334CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    LEVY MN, IMPERIAL ES (1961) Oxygen shunting in renal cortical and medullary capillaries. Am J Physiol 200:159–162PubMedGoogle Scholar
  4. 4.
    Fine LG, Bandyopadhay D, Norman JT (2000) Is there a common mechanism for the progression of different types of renal diseases other than proteinuria? Towards the unifying theme of chronic hypoxia. Kidney Int Suppl 75:S22–S26CrossRefPubMedGoogle Scholar
  5. 5.
    Jiang J, Nakashima T, Liu KJ, Goda F, Shima T, Swartz HM (1996) Measurement of PO2 in liver using EPR oximetry. J Appl Physiol 80(2):552–558CrossRefPubMedGoogle Scholar
  6. 6.
    Khan N, Mupparaju S, Hou H, Williams BB, Swartz H (2012) Repeated assessment of orthotopic glioma pO(2) by multi-site EPR oximetry: a technique with the potential to guide therapeutic optimization by repeated measurements of oxygen. J Neurosci Methods 204(1):111–117CrossRefPubMedGoogle Scholar
  7. 7.
    Khan N, Li H, Hou H, Lariviere JP, Gladstone DJ, Demidenko E, Swartz HM (2009) Tissue pO2 of orthotopic 9L and C6 gliomas and tumor-specific response to radiotherapy and hyperoxygenation. Int J Radiat Oncol Biol Phys 73(3):878–885CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Stephanie Franzén
    • 1
    • 2
  • Liselotte Pihl
    • 1
  • Nadeem Khan
    • 3
  • Fredrik Palm
    • 1
    • 2
    • 4
  • Håkan Gustafsson
    • 2
    • 5
  1. 1.Division of Drug Research, Experimental Renal Medicine, Department of Medical and Health SciencesLinköping UniversityLinköpingSweden
  2. 2.Center of Medical Image Science and VisualizationLinköping UniversityLinköpingSweden
  3. 3.EPR Center for Viable Systems, Department of RadiologyGeisel School of MedicineHanoverUSA
  4. 4.Division of Integrative Physiology, Department of Medical Cell BiologyUppsala UniversityUppsalaSweden
  5. 5.Department of Biomedical Engineering (MTÖ), County Council of Östergötland, Radiation Physics, Department of Medical and Health SciencesLinköping UniversityLinköpingSweden

Personalised recommendations