What We Learn from In Vivo EPR Oxygen Images

Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 812)

Abstract

Distributions of oxygen concentration (pO2) are a critical determinant of normal tissue health as well as tumor aggressiveness and response to therapy. A number of studies show the value of normal tissue and tumor tissue oxygenation images and some of these will be discussed here. A strong correlation between tumor hypoxic fraction as measured with electron paramagnetic resonance oxygen imaging and radiation treatment success or failure has been found in two separate cancer types. Oxygen images of the torso of wild type mice show initial reduction of lung, liver, visceral, and muscle pO2 with cyclic halving of fraction of inspired oxygen (FiO2), but variation is blunted over an hour. Spontaneous breast cancers in Mouse Mammary Tumor Viral (MMTV) promoted-polyoma middle T antigen (PyMT) mice with BNIP3, a major factor in promotion of mitochondrial autophagy, knocked out will be compared with wild type animals. Preliminary studies for the BNIP3 knock out animals show extremely low pO2. The wide variety of studies, in which oxygen images can play an integral role, serve to demonstrate the importance of oxygen images.

Keywords

EPR Oxygen imaging Hypoxia BNIP3 Cancer Microenvironment response 

Notes

Acknowledgments

Supported by NIH grants P41 EB002034 and R01 CA98575.

References

  1. 1.
    Hockel M, Schlenger K, Aral B et al (1996) Association between tumor hypoxia and malignant progression in advanced cancer of the uterine cervix. Cancer Res 56:4509–4515PubMedGoogle Scholar
  2. 2.
    Shannon AM, Bouchier-Hayes DJ, Condron CM et al (2003) Tumour hypoxia, chemotherapeutic resistance and hypoxia-related therapies. Cancer Treat Rev 29:297–307CrossRefPubMedGoogle Scholar
  3. 3.
    Carmeliet P, Dor Y, Herbert JM et al (1998) Role of HIF-1alpha in hypoxia-mediated apoptosis. Nature 394:485–490CrossRefPubMedGoogle Scholar
  4. 4.
    Rofstad EK (2000) Microenvironment-induced cancer metastasis. Int J Radiat Biol 76:589–605CrossRefPubMedGoogle Scholar
  5. 5.
    Dewhirst MW, Klitzman B, Braun RD et al (2000) Review of methods used to study oxygen transport at the microcirculatory level. Int J Cancer 90:237–255CrossRefPubMedGoogle Scholar
  6. 6.
    Zhao DW, Jiang L, Mason RP (2004) Measuring changes in tumor oxygenation. Methods Enzymol 386:378–418CrossRefPubMedGoogle Scholar
  7. 7.
    Tatum JL (2006) Hypoxia: importance in tumor biology, noninvasive measurement by imaging, and value of its measurement in the management of cancer therapy. Int J Rad Biol 82:699–757CrossRefPubMedGoogle Scholar
  8. 8.
    Bayer C, Vaupel P (2012) Acute versus chronic hypoxia in tumors: controversial data concerning time frames and biological consequences. Strahlenther Onkol 188:616–627CrossRefPubMedGoogle Scholar
  9. 9.
    Golman K, Petersson JS, Ardenkjaer-Larsen JH et al (2000) Dynamic in vivo oxymetry using overhauser enhanced MR imaging. J Magn Reson Imaging 12:929–938CrossRefPubMedGoogle Scholar
  10. 10.
    Elas M, Ahn KH, Parasca A et al (2006) Electron paramagnetic resonance oxygen images correlate spatially and quantitatively with oxylite oxygen measurements. Clin Cancer Res 12:4209–4217CrossRefPubMedGoogle Scholar
  11. 11.
    Elas M, Bell R, Hleihel D et al (2008) Electron paramagnetic resonance oxygen image hypoxic fraction plus radiation dose strongly correlates with tumor cure in FSa fibrosarcomas. Int J Radiat Oncol Biol Phys 71:542–549CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Elas M, Magwood JM, Butler B et al (2013) EPR oxygen images predict tumor control by a 50 percent tumor control radiation dose. Cancer Res (online and in press)Google Scholar
  13. 13.
    Tracy K, Macleod KF (2007) Regulation of mitochondrial integrity, autophagy and cell survival by BNIP3. Autophagy 3(6):616–619CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Gage Redler
    • 1
    • 2
  • Boris Epel
    • 1
    • 2
  • Howard J. Halpern
    • 1
    • 2
    • 3
  1. 1.Center for EPR Imaging In Vivo PhysiologyChicagoUSA
  2. 2.Department of Radiation OncologyUniversity of ChicagoChicagoUSA
  3. 3.MC1105, Department of Radiation and Cellular OncologyUniversity of Chicago Medical CenterChicagoUSA

Personalised recommendations