Real-Time, In Vivo Determination of Dynamic Changes in Lung and Heart Tissue Oxygenation Using EPR Oximetry

  • Brian K. Rivera
  • Shan K. Naidu
  • Kamal Subramanian
  • Matthew Joseph
  • Huagang Hou
  • Nadeem Khan
  • Harold M. Swartz
  • Periannan Kuppusamy
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 812)

Abstract

The use of electron paramagnetic resonance (EPR) oximetry for oxygen measurements in deep tissues (>1 cm) is challenging due to the limited penetration depth of the microwave energy. To overcome this limitation, implantable resonators, having a small (0.5 mm diameter) sensory loop containing the oxygen-sensing paramagnetic material connected by a pair of twisted copper wire to a coupling loop (8–10 mm diameter), have been developed, which enable repeated measurements of deep-tissue oxygen levels (pO2, partial pressure of oxygen) in the brain and tumors of rodents. In this study, we have demonstrated the feasibility of measuring dynamic changes in pO2 in the heart and lung of rats using deep-tissue implantable oxygen sensors. The sensory loop of the resonator contained lithium octa-n-butoxynaphthalocyanine (LiNc-BuO) crystals embedded in polydimethylsiloxane (PDMS) polymer and was implanted in the myocardial tissue or lung pleura. The external coupling loop was secured subcutaneously above chest. The rats were exposed to different breathing gas mixtures while undergoing EPR measurements. The results demonstrated that implantable oxygen sensors provide reliable measurements of pO2 in deep tissues such as heart and lung under adverse conditions of cardiac and respiratory motions.

Keywords

Oximetry Electron paramagnetic resonance (EPR) Implantable oxygen sensors Myocardial tissue Lung pleura 

Notes

Acknowledgments

This work was supported by National Institutes of Health (NIH) grant 5R01EB004031.

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Brian K. Rivera
    • 1
  • Shan K. Naidu
    • 1
  • Kamal Subramanian
    • 1
  • Matthew Joseph
    • 1
  • Huagang Hou
    • 2
  • Nadeem Khan
    • 2
  • Harold M. Swartz
    • 3
  • Periannan Kuppusamy
    • 2
  1. 1.Department of Internal MedicineDavis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterColumbusUSA
  2. 2.EPR Center for the Study of Viable Systems, Department of RadiologyGeisel School of Medicine at DartmouthLebanonUSA
  3. 3.EPR Center for the Study of Viable SystemsThe Geisel School of Medicine at DartmouthLebanonUSA

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