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Single-Cell Microfluidic Cytometry for Next-Generation High-Throughput Biology and Drug Discovery

Chapter
Part of the SpringerBriefs in Cell Biology book series (SBCB)

Abstract

The cell is the smallest unit of life. Commensurate with its importance in biology and medicine, it has traditionally been the focus of technologies seeking to add to our understanding of physiological processes relating to life, death, and disease. Over the past decade, our understanding of cellular complexity has been bolstered by the advent of increasingly precise techniques for the investigation of cellular phenomena. Microfluidic cell cytometry combines analysis on the single cell level with integrated separation and processing techniques and has emerged as one of the most powerful techniques in this context. This chapter will encompass an overview of the current landscape and novel trends as well as challenges facing the technologies in this new field.

Keywords

Circulate Tumor Cell High Throughput Screening PDMS Membrane High Content Screening Microwell Array 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

CCD

Charge-coupled device

CD

Cluster of differentiation

CDR

Complementarity determining region

CMOS

Complementary metal-oxide semiconductor

CTC

Circulating tumor cell

DC

Deformability cytometry

DEAL

DNA-encoded antibody libraries

DNA

Deoxyribonucleic acid

ELISA

Enzyme-linked immunosorbent assay

EMT

Epithelial to mesenchymal transition

EpCAM

Epithelial cell adhesion molecule

FOV

Field of view

HAI

Hospital-acquired infection (also healthcare-associated infection)

HCS

High-content screening

HIV

Human immunodeficiency virus

HTS

High-throughput screening

IFNγ

Interferon gamma

ISAAC

Immuno-spot array assay chip

MCF7

Michigan Cancer Foundation-7

MET

Mesenchymal to epithelial transition

MITOMI

Mechanically induced trapping of molecular interaction

PCR

Polymerase chain reaction

PMA

Phorbol 12-myristate 13-acetate

POC

Point-of-care

RNA

Ribonucleic acid

RT-PCR

Reverse transcription polymerase chain reaction

SCBC

Single-cell barcode chip

Simoa

Single molecule array

STEAM

Serial time-encoded amplified microscopy

TF

Transcription factor

TNFα

Tumor necrosis factor alpha

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Copyright information

© Ella Palmer 2014

Authors and Affiliations

  1. 1.Department of BioengineeringUniversity of CaliforniaLos AngelesUSA
  2. 2.Molecular Screening Shared ResourcesCalifornia NanoSystems InstituteLos AngelesUSA

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