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Focused Ion Beam Technology as a Fabrication and Inspection Tool in Neuron Interfacing

  • Leonardo SileoEmail author
  • Ferruccio Pisanello
  • Luigi Martiradonna
  • Massimo De Vittorio
Chapter

Abstract

Recent trends in the development of devices for electrophysiology involve the fabrication of electrodes with three-dimensional micro- and nanoprotrusions. These devices take advantage of the natural capacity of cells to actively interact with nanostructured substrates in order to realize a more intimate cell-to-electrode coupling. In this chapter, we review the use of focused ion beam (FIB) technology as a versatile tool for fabricating nanostructures of different shape and size on top of freely chosen substrates. This approach allows custom design and fabrication of nanoprotrusions to optimize cell-to-electrode electrical coupling, while at the same time allowing leeway to optimize the microelectronic substrate. Examples of enhanced interaction of cells with nanostructures are reviewed, with respect to nanoprotrusion geometry and surface functionalization, to illustrate the potential of FIB-based deposition as a tool for realizing new types of nanostructures for neurophysiological measurements. Finally, the combined focused ion beam/scanning electron microscope is presented as a tool for investigating the physical basis for interactions between neuronal cell membranes and nanostructured surfaces.

Keywords

Electrical Coupling Extracellular Recording Multi Electrode Array Aplysia Neuron Nanostructured Substrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Leonardo Sileo
    • 1
    Email author
  • Ferruccio Pisanello
    • 1
  • Luigi Martiradonna
    • 1
  • Massimo De Vittorio
    • 1
    • 2
  1. 1.Center for Biomolecular Nanotechnologies @UNILE, IITArnesanoItaly
  2. 2.NNL-National Nanotechnology LaboratoryCNR-NANO, Università del SalentoLecceItaly

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