Derivation of Induced Pluripotent Stem Cells
Chapters 5 and 6 looked at human induced pluripotent stem cell (hiPSC) lines growing in feeder-based and feeder-free systems. This chapter reviews the derivation of hiPSCs. The various delivery systems have pros and cons that must be taken into account when generating new iPSCs for specific purposes. Not all delivery systems work on all cell types. Some delivery systems have a residual effect on the host genome caused by the delivery system itself, as is the case for retroviral vectors. To date, the methods of choice are those that use agents that do not integrate into the host genome. These include Sendai viral vectors, episomal vectors, protein transduction and RNA-based transfection.
KeywordsReprogramming Vector Messenger RNA Fibroblast
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