Derivation of Induced Pluripotent Stem Cells

  • Lyn Healy
  • Ludmila Ruban
Chapter

Abstract

Chapters  5 and  6 looked at human induced pluripotent stem cell (hiPSC) lines growing in feeder-based and feeder-free systems. This chapter reviews the derivation of hiPSCs. The various delivery systems have pros and cons that must be taken into account when generating new iPSCs for specific purposes. Not all delivery systems work on all cell types. Some delivery systems have a residual effect on the host genome caused by the delivery system itself, as is the case for retroviral vectors. To date, the methods of choice are those that use agents that do not integrate into the host genome. These include Sendai viral vectors, episomal vectors, protein transduction and RNA-based transfection.

Keywords

Reprogramming Vector Messenger RNA Fibroblast 

Suggested Reading

  1. Anokye-Danso F, Trivedi CM, Juhr D, Gupta M, Cui Z, Tian Y, et al. Highly efficient miRNA-mediated reprogramming of mouse and human somatic cells to pluripotency. Cell Stem Cell. 2011;8:376–88.PubMedCentralPubMedCrossRefGoogle Scholar
  2. Chang CW, Lai YS, Pawlik KM, Liu K, Sun CW, Li C, et al. Polycistronic lentiviral vector for “hit and run” reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Stem Cells. 2009;27:1042–9.PubMedCrossRefGoogle Scholar
  3. David L, Polo JM. Phases of reprogramming. Stem Cell Res. 2014;12:754–61.PubMedCrossRefGoogle Scholar
  4. Fusaki N, Ban H, Nishiyama A, Saeki K, Hasegawa M. Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome. Proc Jpn Acad Ser B Phys Biol Sci. 2009;85:348–62.PubMedCentralPubMedCrossRefGoogle Scholar
  5. Hu K. Vectorology and factor delivery in induced pluripotent stem cell reprogramming. Stem Cells Dev. 2014. doi: 10.1089/scd.2013.0621.Google Scholar
  6. Jacobs JP, Garrett AJ, Meron R. Characteristics of a serially propagated human diploid cell designated MRC-9. J Biol Stand. 1979;7:113–22.PubMedCrossRefGoogle Scholar
  7. Kim D, Kim CH, Moon JI, Chung YG, Chang MY, Han BS, et al. Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell. 2009;4:472–6.PubMedCentralPubMedCrossRefGoogle Scholar
  8. Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, et al. A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Sci Rep. 2014;4:3594. doi: 10.1038/srep03594.PubMedCentralPubMedGoogle Scholar
  9. Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, et al. An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells. Stem Cells. 2013;31:458–66.PubMedCrossRefGoogle Scholar
  10. Subramanyam D, Lamouille S, Judson RL, Liu JY, Bucay N, Derynck R, Blelloch R. Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells. Nat Biotechnol. 2011;29:443–8.PubMedCentralPubMedCrossRefGoogle Scholar
  11. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007;131:861–72.PubMedCrossRefGoogle Scholar
  12. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006;126:663–76.PubMedCrossRefGoogle Scholar
  13. Tavernier G, Mlody B, Demeester J, Adjaye J, De Smedt SC. Current methods for inducing pluripotency in somatic cells. Adv Mater. 2013;25:2765–71.PubMedCrossRefGoogle Scholar
  14. Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell. 2010;7:618–30.PubMedCentralPubMedCrossRefGoogle Scholar
  15. Warren L, Ni Y, Wang J, Guo X. Feeder-free derivation of human induced pluripotent stem cells with messenger RNA. Sci Rep. 2012;2:657. doi: 10.1038/srep00657.PubMedCentralPubMedCrossRefGoogle Scholar
  16. Warren L, Wang J. Feeder-free reprogramming of human fibroblasts with messenger RNA. Curr Protoc Stem Cell Biol. 2013;27:4A.6.1–27. doi: 10.1002/9780470151808.sc04a06s27.Google Scholar
  17. Zhou H, Wu S, Joo JY, Zhu S, Han DW, Lin T, et al. Generation of induced pluripotent stem cells using recombinant proteins. Cell Stem Cell. 2009;4:381–4.PubMedCrossRefGoogle Scholar
  18. Zhou W, Freed CR. Adenoviral gene delivery can reprogram human fibroblasts to induced pluripotent stem cells. Stem Cells. 2009;27:2667–74.PubMedCrossRefGoogle Scholar
  19. Zhou YY, Zeng F. Integration-free methods for generating induced pluripotent stem cells. Genomics Proteomics Bioinformatics. 2013;11:284–7.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Lyn Healy
    • 1
  • Ludmila Ruban
    • 2
  1. 1.United Kingdom Stem Cell BankNational Institute for Biological Standards and ControlPotters BarUK
  2. 2.Department of Biochemical Engineering Faculty of Engineering ScienceUniversity College LondonLondonUK

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