Gas Chromatographic Columns

  • Herbert H. Wotiz
  • Stanley J. Clark

Abstract

An idealized chromatogram of a pure compound is shown in Figure 3–1. Injection is made at point A, and any material in the sample, such as air, that is not soluble in the stationary phase is swept through the column at the same speed as the carrier gas, emerging at point B. A compound soluble in the stationary phase will travel more slowly, emerging at C. The time from the point of injection to the peak maximum is the retention time t r (AC in Figure 3–1), and the volume of gas passed through the column during this time is the retention volume V r . The quantities are related as follows:
$$ {t_t} = \frac{{{V_t}}}{{{f_{av}}}} $$
where f av is the average flow rate of gas through the column. In most applications, flow rate is held constant so that measuring retention time on the recorder chart is equivalent to measuring retention volume. The chromatogram illustrated is an example of a differential chromatogram. This form of display is virtually universal and will be treated here to the exclusion of all others.

Keywords

Petrol Stein Reten Unifo 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. J. P. Martin and R. L. M. Synge, Biochem. J. 35:1358 (1941).Google Scholar
  2. 2.
    A. T. James and A. J. P. Martin, Biochem. J. 50:679 (1952).Google Scholar
  3. 2a.
    A. A. Patti and A. A. Stein, Steroid Analysis by Gas Liquid Chromatography (Charles C Thomas, Springfield, Illinois, 1964).Google Scholar
  4. 3.
    J. J. van Deemter, F. J. Zuiderweg, and A. Klinkenberg, Chem. Eng. Sci. 5:271 (1956).CrossRefGoogle Scholar
  5. 4.
    J. D. Cheshire and R. P. W. Scott, J. Inst. Petrol. 44:74 (1958).Google Scholar
  6. 5.
    R. P. W. Scott, Gas Chromatography, edited by D. H. Desty (Butter worths, London, 1958), p. 189.Google Scholar
  7. 6.
    J. H. Purnell, Gas Chromatography (John Wiley & Sons, Inc., New York, 1962), p. 149.Google Scholar
  8. 7.
    J. H. Purnell, Gas Chromatography (John Wiley & Sons, Inc., New York, 1962), p. 136.Google Scholar

Copyright information

© Springer Science+Business Media New York 1966

Authors and Affiliations

  • Herbert H. Wotiz
    • 1
  • Stanley J. Clark
    • 1
  1. 1.School of MedicineBoston UniversityBostonUSA

Personalised recommendations