Skip to main content

Applications of Light and Electron Microscopic Autoradiography to the Study of Cytogenesis of the Forebrain

  • Chapter

Abstract

In the early stages of ontogeny, the wall of the prosencephalon, later developing into highly specialized structures is still made up of relatively simple medullary epithelium, as are the other portions of the neural tube. No neuroblast appears yet in this stage. Recent investigations (21) have elucidated in detail the functional and morphological characteristics of the matrix cell that constitutes the medullary epithelium.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adrian, E. K., B. E. Walker: Incorporation of thymidine-H3 by cells in normal and injured mouse spinal cord. J. Neuropath. exper. Neurol. 21 (1962), 597–609.

    Article  Google Scholar 

  2. Angevine, J. B. : Autoradiographic study of histogenesis in the area dentata of the cerebral cortex in the mouse. Anat. Rec. 148 (1964), 225.

    Google Scholar 

  3. Angevine, J. B., R. L. Sidman: Autoradiographic study of cell migration during histogenesis of cerebral cortex in the mouse. Nature 192 (1961), 766–768.

    Article  PubMed  Google Scholar 

  4. Atlas, M., V. P. Bond: The cell generation cycle of the eleven-day mouse embryo, J. Cell. Biol. 26 (1965), 19–24

    Article  PubMed  CAS  Google Scholar 

  5. Bailey, P., H. Ctjshing: Tumors of the Glioma Group. Lippincott, Philadelphia 1926.

    Google Scholar 

  6. Berry, M., A. W. Rogers: Histogenesis of mammalian neocortex. In: Hassler, R., H. Stephan (ed. ): Evolution of the Forebrain. Thieme, Stuttgart, 1966, 197–205.

    Google Scholar 

  7. DeLong, G. R., R. L. Sidman: Effects of eye removal at birth on histogenesis of the mouse superior colliculus. An autoradiographic analysis with tritiated thymidine, J. comp. Neurol. 118 (1962), 205–224.

    Article  PubMed  CAS  Google Scholar 

  8. Fahrquhar, M. G., J. F. Hartmann: Neuroglial structure and relationships as revealed by electron microscopy, J. Neuropath. exper. Neurol. 16 (1957), 18–39.

    Article  Google Scholar 

  9. Fujita, H., S. Fujita: Electron microscopic studies on neuroblast differentiation in the central nervous system of domestic fowl, Z. Zellforsch. 60 (1963), 463–478.

    Article  PubMed  CAS  Google Scholar 

  10. Fujita, H., S. Fujita: Electron microscopic observations on the histogenesis (I) On the nerve cell differentiation. Acta anat. Nippon. 38 (1963), 85–94.

    PubMed  CAS  Google Scholar 

  11. Fujita, H., S. Fujita: Electron microscopic studies on the histogenesis of the central nervous system of the domestic fowl (II), Neuroglia, Acta anat. Nippon. 38 (1963), 95–108.

    CAS  Google Scholar 

  12. Fujita, H., S. Fujita: Electron microscopic studies on the differentiation of the ependymal cells and the glioblasts in the spinal cord of domestic fowl. Z. Zellforsch. 64. (1964), 262–272.

    Google Scholar 

  13. Fujita, H., J. Ochi, N. Otsuka, S. Fujita: Some problems on the histochemical studies of the nervous tissue, Rec. Adv. Res. Nerv. System (Tokyo) 8 (1964), 471–484.

    Google Scholar 

  14. Fujita, S. : Medulloepithelioma: Its place in the histogenetic classification of neurectodermal tumors. Acta Pathol. Japon. 8 (1958), 789–794.

    Google Scholar 

  15. Fujita, S. : Mitotic pattern and histogenesis of the central nervous system, Nature 185 (1960), 702–703.

    Article  PubMed  CAS  Google Scholar 

  16. Fujita, S. : Kinetics of cellular proliferation. Exper. Cell Research 28 (1962), 52–60.

    Article  CAS  Google Scholar 

  17. Fujita, S. : The matrix cell and cytogenesis in the developing central nervous system, J. comp. Neurol. 120 (1963), 37–42.

    Article  PubMed  CAS  Google Scholar 

  18. Fujita, S. : Histogenesis of the central nervous system and classification of neurectodermal tumors. Rec. Adv. Res. Nerv. System (Tokyo) 7 (1963), 117–143.

    Google Scholar 

  19. Fujita, S. : Analysis of neuron differentiation in the central nervous system by tritiated thymidine autoradiography, J. comp. Neurol. 122 (1964), 311–328.

    Article  PubMed  CAS  Google Scholar 

  20. Fujita, S. : An autoradiographic study on the origin and fate of the sub-pial glioblast in the embryonic chick spinal cord, J. comp. Neurol. 124 (1965), 51–60.

    Article  PubMed  CAS  Google Scholar 

  21. Fujita, S. : The matrix cell and histogenesis of the central nervous system, Laval Méd. 36 (1965), 125–130.

    PubMed  CAS  Google Scholar 

  22. Fujita, S., M. Horii: Analysis of cytogenesis in chick retina by tritiated thymidine autoradiography, Arch. Histol. Japon. 23 (1963), 359–366.

    Article  CAS  Google Scholar 

  23. Fujita, S., M. Horii, T. Tanimura, H. Nishimura: H3-thymidine autoradiographic studies on cytokinetic responses to X-ray irradiation and to thio-TEPA in the neural tube of mouse embryos, Anat. Rec. 149 (1964), 37–48.

    Article  PubMed  CAS  Google Scholar 

  24. Fujita, S., A. Kojima, H. Kaku: Analysis of cellular differentiation and proliferation in the central nervous system by H3-thymidine autoradiography, Proc. Japan Conf. Radioisotopes 4 (1963), 178–180.

    Google Scholar 

  25. Fujita, S., H. Mio, J. Kikkawa, M. Tegoshi, M. Omatsu: Cytoplasmic changes during mitosis, J. Kyoto Pref. Univ. Med. 68 (1960), 1001–1007.

    Google Scholar 

  26. Fujita, S., S. Miyake: Selective labeling of cell groups and its application to cell identification, Exper. Cell Res. 28 (1962), 158–161.

    Article  CAS  Google Scholar 

  27. Fujita, S., Y. Omatsu, H. Mio, T. Morikubo, J. Kikkawa: Mitotic pattern of epithelium in situ and in vitro, J. Kyoto Pref. Univ. Med. 68 (1960), 950–953.

    Google Scholar 

  28. Fujita, S., M. Shimada: Cellular proliferation in the brain of early postnatal mouse. In preparation.

    Google Scholar 

  29. Fujita, S., K. Suwa, M. Tegoshi, S. Miyake, O. Takeoka: Emigration of mitotic cells in single-layered cylindrical epithelium and neural tube: Its relationship to a new concept of the cytogenesis of the central nervous system, Acta pathol. Japon. 10 (1960), 202.

    Google Scholar 

  30. Ftjjita, S., O. Takeoka: Identity of “Keimzellen von His” and primitive spongioblast revealed by colchicine technique and trypaflavin administration, Acta pathol. Japon. 11 (1961), 256–257.

    Google Scholar 

  31. Ftjjita, S., O. Takeoka: Morphologic and quantitative studies on the cellular proliferation and differentiation in the central nervous system of human embryo, Acta pathol. Japon. 11 (1961), 256.

    Google Scholar 

  32. Fujita, S., O. Takeoka: Studies of gliogenesis using tritiated thymidine autoradiography, electron microscopy and silver impregnation techniques, with special references to the origin and fate of Hortega’s microglioblasts. Trans. Soc. Pathol. Japon. 53 (1964), 192–194.

    Google Scholar 

  33. Hicks, S. P., C. J. D’Amato, MA. Coy, E. D. O’Brien, J. M. Thurston, D. L. Jointes: Migrating cells in the developing central nervous system studied by their radiosensitivity and tritiated thymidine uptake. In: Fundamental Aspects of Radiosensitivity. Brookhaven Symposium in Biology No. 14, Upton, New York 1961.

    Google Scholar 

  34. Hicks, S. P., M. C. Cavanaugh, E. D. O’Brien: Effects of anoxia on the developing cerebral cortex in the rat. Amer. J. Path. 40 (1962), 615–635.

    PubMed  CAS  Google Scholar 

  35. His, W. : Die Neuroblasten und deren Entstehung im embryonalen Mark. Z. Anat. Entwicklungsgesch. (1889), 249–300.

    Google Scholar 

  36. Hortega, Del Rio P. : Microglia. In: Penfield’s Cytology and Cellular Pathology of the Nervous System. Vol. 2, 483–534. Paul P. Hoeber, New York 1932.

    Google Scholar 

  37. Kershman, J. : The medulloblastoma and neuroblastoma: A study of human embryos, Arch. Neurol. Psychiat. 40 (1938), 937–967.

    Article  Google Scholar 

  38. Kershman, J. : Genesis of microglia in the human brain, Arch. Neurol. Psychiat. 41 (1939), 24–50.

    Article  Google Scholar 

  39. Konigsmark, B. W., R. L. Sidman: Origin of gitter cells in the mouse brain, J. Neuropath. exper. Neurol. 22 (1963), 327–328.

    Google Scholar 

  40. Konigsmark, B. W., R. L. Sidman: Origin of Macrophages in the mouse. J. Neuropath. exper. Neurol. 22 (1963), 643–676.

    Article  CAS  Google Scholar 

  41. Kosunen, T. U., B. H. Wacksman: Radioautographic studies of experimental allergic encephalomyelitis (EAE) in rats, J. Neuropath. exper. Neurol. 22 (1963), 324–326.

    Google Scholar 

  42. Kosunen, T. U., B. H. Waksman, I. K. Samuelson: Radio-autographic study of cellular mechanisms in delayed hypersensitivity. II. Experimental allergic encephalomyelitis in the rat, J. Neuropath, exper. Neurol. 22 (1963), 367–380.

    Article  CAS  Google Scholar 

  43. Langman, J. : The formation of the spinal cord in the chick embryo, examined by means of radioautography. 8th Int. Congr. Anatomists, Wiesbaden, p. 69 (1965).

    Google Scholar 

  44. Cf. Martin, A., J. Langman, The development of the spinal cord examined by autoradiography. J. Embryol. exp. Morphol. 14 (1965), 25–35.

    PubMed  CAS  Google Scholar 

  45. Lyser, K. M. : Early differentiation of motor neuroblast in the chick embryo as studied by electron microscopy. I. General aspects., Developmental Biol. 10 (1964), 433–466.

    Article  CAS  Google Scholar 

  46. Meller, K., W. Wechsler: Elektronmikroskopische Befunde am Ependym des sich entwickelnden Gehirns von Hühnerembryonen, Acta Neuropath. 3 (1964), 609–626

    Article  Google Scholar 

  47. Metz, A., H. Spatz: Die Hortegaschen Zellen (= das sogenannte dritte Element) und über ihre funktionelle Bedeutung, Z. ges. Neurol. 89 (1924), 138–170.

    Article  Google Scholar 

  48. Miale, I. L., R. L. Sidman: An autoradiographic analysis of histogenesis in the mouse cerebellum, Exper. Neurol. 4 (1961), 277–296.

    Article  CAS  Google Scholar 

  49. Miyake, S., K. Araki, M. Sugahara, S. Ftjjita: Growth pattern of “matrix cells” of the central nervous system and formation of tubular structure simulating neural tube in cultures of human fetal brain, Arch. Histol. Japon. 22 (1961), 117–121.

    Article  Google Scholar 

  50. Penfield, W. : Neuroglia, normal and pathological. In: Penfield’s Cytology and Cellular Pathology of the Nervous System. Vol. 2, 421–479. P. Hoeber, New York 1932.

    Google Scholar 

  51. Przybylski, R. J. : Electron microscope autoradiography, Exper. Cell Res. 24 (1961), 181–184.

    Article  CAS  Google Scholar 

  52. Salpeter, M. M., L. Bachman: Autoradiography with the electron microscope. A procedure for improving resolution, sensitivity, and contrast, J. Cell. Biol. 22 (1964), 469–477.

    Article  PubMed  CAS  Google Scholar 

  53. Sauer, F. C. : Mitosis in the neural tube, J. comp. Neurol. 62 (1935), 377–405.

    Article  Google Scholar 

  54. Sauer, M. E., A. C. Chittenden: Desoxyribonucleic acid content of cell nuclei in the neural tube of the chick embryo; evidence for intermitotic migration of nuclei, Exper Cell Res. 16 (1959), 1–6.

    Article  CAS  Google Scholar 

  55. Sauer, M. E., B. E. Walker: Radioautographic study of interkinetic nuclear migration in the neural tube, Proc. Soc. exp. Biol. 101 (1959), 557–560.

    Article  PubMed  CAS  Google Scholar 

  56. Schaper, A. : Die frühesten Differenzierungsvorgänge im Centralnervensystem, Roux’ Arch. Entwickl. -Mech. 5 (1897), 430 bis 431.

    Google Scholar 

  57. Schaper, A. : The earliest differentiation in the central nervous system of vertebrates, Science 5 (1897), 430–31.

    Google Scholar 

  58. Sidman, R. L., I. L. Miale, N. Feder: Cell proliferation and migration in the primitive ependymal zone ; an autoradiographic study of histogenesis in the nervous system, Exper. Neurol. 1 (1959), 322–333.

    Article  CAS  Google Scholar 

  59. Sidman, R. L. : Histogenesis of mouse retina studied with thymidine-3H. In: Smelser, O. K. : The Structure of the Eye, pp. 487 to 506. Academic Press, New York 1961.

    Google Scholar 

  60. Smart, I. : The location of mitosis-capable neuron precursor cells in the mouse neural epithelium, J. Anat. 99 (1965), 212.

    Google Scholar 

  61. Smart, I., O. P. Leblond: Evidence for division and transformations of neuroglia cells in the mouse brain, as derived from radioautpgraphy after injection of thymidine-H3, J. comp. Neurol. 116 (1961), 349 to 367.

    Article  Google Scholar 

  62. Tennyson, V. M. : Electron microscopic observations of the development of the neuroblast in the rabbit embryo. Proc. Intern. Congr. Electron Microscopy, 5th, Philadalphia, Vol. II, p. N-8. Academic Press, New York 1962.

    Google Scholar 

  63. Tennyson, V. M., G. D. Pappas: An electron microscope study of ependymal cells of the fetal, early postnatal and adult rabbit, Z. Zeilforsch. 56 (1962), 595–618.

    Article  CAS  Google Scholar 

  64. Uzman, L. L. : The histogenesis of the mouse cerebellum as studied by its tritiated thymidine uptake, J. comp. Neurol. 114 (1960), 137–160.

    Article  PubMed  CAS  Google Scholar 

  65. Watterson, R. L., P. Veneziano, A. Bertha: Absence of a true germinal zone in neural tubes of young chick embryos as demonstrated by colchicine technique, Anat. Rec. 124 (1956), 379.

    Google Scholar 

  66. Wechsler, W. : Elektronmikroskopische Untersuchung der Entwicklung von Nervenzellen und Nervenfasern bei Hühnerembryonen, Anat. Anz. 115 (1965), 287–302.

    CAS  Google Scholar 

  67. Yamamoto, Y., S. Fujita, H. Mio, J. Kikkawa, S. Miyake, O. Takeoka: Ontogenetic and phylogenetic study on the distribution of germinal cells and ependymal cells in the central nervous system, Acta pathol. Japon. 10 (1960), 202–203.

    Google Scholar 

  68. Yamamoto, Y., H. Mio, J. Kikkawa, M. Tegoshi, Y. Omatsu, Y. Okumura, S. Fujita: Morphological studies on the distribution of germinal cells in the central nervous system of Gecko japonicus, J. Kyoto Pref. Univ. Med. 68 (1960), 1544–1547.

    Google Scholar 

  69. Yamamoto, Y., Y. Omatsu, H. Mio, J. Kikkawa, Y. Okumura, M. Tegoshi, S. Fujita: Morphological studies on the distribution of germinal cells in the central nervous system of bony fish, J. Kyoto Pref. Univ. Med. 68 (1960), 1617–1621.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1966 Springer Science+Business Media New York

About this chapter

Cite this chapter

Fujita, S. (1966). Applications of Light and Electron Microscopic Autoradiography to the Study of Cytogenesis of the Forebrain. In: Hassler, R., Stephan, H. (eds) Evolution of the Forebrain. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-6527-1_17

Download citation

  • DOI: https://doi.org/10.1007/978-1-4899-6527-1_17

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-6245-4

  • Online ISBN: 978-1-4899-6527-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics