Sudden Death in Germfree Mice Reared Through Successive Generations on Chemically Defined Liquid Diet
A diet for germfree animals which consists of chemically defined, low-molecular-weight nutrients, and which can be prepared in liquid form for sterilization by filtration, brings the experimental animal one step farther toward complete control of nutritional and antigenic variables. Such a diet, consisting of purified amino acids, sugars, ethyl linoleate, vitamins and minerals, all dissolved in a single water solution, has been fed to germfree rats (1) and to germfree mice (2,3). The fat-soluble portion of the diet had been solubilized in the water solution by use of Polysor-bate 80, These preliminary experiments had implicated the nonionic detergent as a cause of persistent diarrhea in some mice. Changes in the lymph nodes of germfree mice fed the detergent diet also resembled those observed by Mori and Kato (4) in conventional rats fed polysorbate. For these reasons the detergent was eliminated and the fat-soluble portion of the diet was prepared and fed separately from the water-soluble portion, according to a procedure reported at the 1966 meeting of this Association. Diet prepared in this way has now been subjected to long-term testing of its nutritional adequacy.
KeywordsSugar Flare Folic Acid Fructose Choline
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