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Ion Transport in the Synaptosome and Na+-K+-ATPase

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Methods of Neurochemistry

Abstract

The availability of techniques for the preparation of synaptosomes from brain homogenates has made possible the morpho-biochemical and neuropharmacological investigation of this functionally important part of the neuron in vitro. Reports from several laboratories have shown that the synaptosomal cytoplasm can carry on a host of metabolic activities (for reviews see Refs. 1–5) which appear to be similar to those of the perikaryon of the neuron. The constituents of the synaptosome, which include the intraneuronal cytoplasm, mitochondria, and synaptic vesicles, are also separated from the outside environment by a permeability barrier with properties characteristic of a neuronal membrane. Recently there has been a crescendo of research on transport Na+-K+-ATPase in the synaptosome, the transport of ions across its limiting membrane, and the effect of various metabolic inhibitirs and pharmacological agents thereon (6).

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  1. Department of Cell and Molecular Biology, Medical College of Georgia, Augusta, Georgia, USA

    Ata A. Abdel-Latif

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  1. Department of Biochemistry, Creighton University Medical School, Omaha, Nebraska, USA

    Rainer Fried

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Abdel-Latif, A.A. (1973). Ion Transport in the Synaptosome and Na+-K+-ATPase. In: Fried, R. (eds) Methods of Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-6375-8_4

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  • DOI: https://doi.org/10.1007/978-1-4899-6375-8_4

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