Virus elimination and testing

  • Mary C. Coleman
  • Wayne Powell


In recent years there has been an increasing awareness of the value of germplasm conservation. As new varieties comprise an increasingly large proportion of cultivated crops, the genetic diversity of major crop species is being eroded. A number of initiatives have been undertaken by international organisations to collect, evaluate and preserve wild and primitive genotypes. For seed-propagated crops this is relatively easy but many crops have to be propagated asexually because the plants do not produce seeds or the crop depends on the performance of a selected genotype. Thus asexually propagated material needs to be maintained clonally by an appropriate means of vegetative propagation.


Potato Virus Plant Virus Virus Elimination Meristem Culture Apical Dome 
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  1. 1.
    Al Moudallal Z, Altschuh D, Briand JP & Van Regenmortel MHV (1984) Comparative sensitivity of different ELISA procedures for detecting monoclonal antibodies. Journal of Immunological Methods 68: 35–43.CrossRefGoogle Scholar
  2. 2.
    Atkinson PH & Matthews REF (1970) On the origin of dark green tissue in tobacco leaves infected with tobacco mosaic virus. Virology 90: 344–356.CrossRefGoogle Scholar
  3. 3.
    Baulcombe D, Flavell RB, Boulton RE & Jellis GJ (1984). The sensitivity and specificity of a rapid nucleic acid hybridisation method for the detection of potato virus X in crude sap samples. Plant Pathology 33: 361–370.CrossRefGoogle Scholar
  4. 4.
    Bittner H, Schenk G, Schuster G & Kluge S (1989) Elimination by chemotherapy of potato virus S from potato plants grown in vitro. Potato Research 32: 175–179.CrossRefGoogle Scholar
  5. 5.
    Brandes J (1957) Eine elektronenmikroskopische Schnellmethode zum Nachweis faden und Stabchenformiger, Viren, insbesondere in Kartoffeldunkelkeimen. Nachr Bt Dr Pflschutzd 9: 157–152.Google Scholar
  6. 6.
    Brunt AA (1985) The production and distribution of virus-tested ornamental bulb crops in England: Principles practice and prognosis. Acta Horticulturae 164: 153–161.Google Scholar
  7. 7.
    Cassells AC & Long RD (1982) The elimination of potato viruses X, Y, S and M in meristem and expiant cultures of potato in the presence of Virazole. Potato Research 25: 165–173.CrossRefGoogle Scholar
  8. 8.
    Clark MF (1981) Immunosorbent assays in plant pathology. Annual Review of Phytopathology 19: 83–106.CrossRefGoogle Scholar
  9. 9.
    Clark MF & Adams AN (1977) Characteristics of the microplate method of enzyme linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34: 475–483.PubMedCrossRefGoogle Scholar
  10. 10.
    Cohen AJ (1986) Plant tissue cell culture abstracts. International Congress 6: 30.Google Scholar
  11. 11.
    De Bokx JA (1972) Viruses of potato and seed-potato production. Wageningen: Centre for Agricultural Publishing and Documentation.Google Scholar
  12. 12.
    Derrick KS (1973) Quantitative assay for plant viruses using serologically specific electron microscopy. Virology 56: 652–653.PubMedCrossRefGoogle Scholar
  13. 13.
    Dodds JA & Bar-Joseph M (1983) Double-stranded RNA from plants infected with clostero viruses. Phytopathology 73: 419–423.CrossRefGoogle Scholar
  14. 14.
    Esau K (1967) Anatomy of plant virus infections. Annual Review of Phytopathology 5: 45–76.CrossRefGoogle Scholar
  15. 15.
    Fulton, R.W. (1951) Superinfection by strains of tobacco mosaic virus. Phytopathology 41, 579–592.Google Scholar
  16. 16.
    Gamborg DL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50: 151–158.PubMedCrossRefGoogle Scholar
  17. 17.
    Gough KA & Shukla DD (1980) Further studies on the use of protein A in immuno electron microscopy for detecting virus particles. Journal of General Virology 51: 415–419.PubMedCrossRefGoogle Scholar
  18. 18.
    Hansen AJ & Lane WD (1985) Elimination of apple chlorotic leafspot virus from apple shoot cultures. Plant Diseases 69: 134–135.CrossRefGoogle Scholar
  19. 19.
    Hiebert E, Purcifull DE, Christie RG & Christie SR (1971) Partial purification of inclusions induced by tobacco etch virus and potato virus Y. Virology 43: 638–646.PubMedCrossRefGoogle Scholar
  20. 20.
    Horvath J (1963) Neuere Beitrage zum Vorkommen von Kartoffelviren mit besonderer Rücksicht auf Komplexinfektionen. Acta Agronomica Academicae Scientiarum Hungarice 14: 67–81.Google Scholar
  21. 21.
    Horvath J (1967) Separation and determination of viruses pathogenic to potato virus Y. Acta Phytopathologica Academiae Scientiarum Hungaricae 2: 319–360.Google Scholar
  22. 22.
    Horvath J (1985) A check-list of new host plants for identification and separation of twelve potato viruses. Potato Research 28: 71–89.CrossRefGoogle Scholar
  23. 23.
    Ikegami M & Fraenkel-Comrat H (1979) Characterisation of double stranded ribonucleic acid in tobacco leaves. Proceedings National Academy of Sciences USA 76: 3637–3640.CrossRefGoogle Scholar
  24. 24.
    Jordan R & Dodds JA (1985) Double-stranded RNA in detection of diseases of known and unproven viral etiology. Acta Horticulture 164: 101–108.Google Scholar
  25. 25.
    Jordan RL, Heick JA, Dodds JA & Ohr H (1983) Rapid detection of sunblotch viroid RNA and virus-like double-stranded RNA in multiple avocado samples. Phytopathology 73: 791.(Abs.)CrossRefGoogle Scholar
  26. 26.
    Kartha KK (1986) Production and indexing of disease-free plants. In: Plant tissue culture and its agricultural application. (LA Withers and PG Alderson, Eds). London, Butterworth Press.Google Scholar
  27. 27.
    Lange L (1986) The practical application of new developments in test procedures for the detection of viruses in seed. In: Developments in Applied Biology 1. Developments and Applications in Virus Testing. Eds RAC Jones and L Torrance. Association of Applied Biologists, Wellesbourne, UK.Google Scholar
  28. 28.
    Lesemann DE & Paul HL (1980) Conditions for the use of protein A in combination with the Derrick method of immuno electron microscopy. Acta Horticulturae 110: 119–128.Google Scholar
  29. 29.
    Long RD & Cassells AC (1986) Elimination of viruses from tissue culture in the presence of antiviral chemicals. In: Plant Tissue Culture and its Agricultural Application (LA Withers and PG Alderson, Eds), Butterworth Press.Google Scholar
  30. 30.
    Matthews REF (1970) Plant Virology, Academic Press, New York.Google Scholar
  31. 31.
    Morris TJ & Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus infected plant and fungal tissue. Phytopathology 69: 854–858.CrossRefGoogle Scholar
  32. 32.
    Morris TJ, Dodds JA, Hillman B, Jordan RL, Lommel SA & Tamalki SJ (1983) Viral specific ds RNA: diagnostic value for plant virus disease identification. Plant Molecular Biology Reporter 1: 27–30.CrossRefGoogle Scholar
  33. 33.
    Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiological Plantarum 15: 473–497.CrossRefGoogle Scholar
  34. 34.
    Muraskishi HH & Carlson PS (1976) Regeneration of virus-free plants from dark green islands of tobacco mosaic virus-infected tobacco leaves. Phytopathology 66: 931–932.CrossRefGoogle Scholar
  35. 35.
    Noordam D (1973) Identification of plant viruses. Methods and experiments. Centre for Agricultural Publishing Documentation, Wageningen.Google Scholar
  36. 36.
    Quak, F. (1977). Meristem culture and virus-free plants. In: Applied and fundamental aspects of plant cell, tissue and organ culture. J Reinert and YPS Bajaj (Eds). Springer-Verlag, Berlin.Google Scholar
  37. 37.
    Roberts IM, Milne RG, Van Regenmortel MHV (1982) Suggested terminology for virus-antibody interactions observed by electron microscopy. Intervirology 18: 147–149.PubMedCrossRefGoogle Scholar
  38. 38.
    Shepard JF (1975) Regeneration of plants from protoplasts of potato virus X infected tobacco leaves. Virology 66: 492–501.PubMedCrossRefGoogle Scholar
  39. 39.
    Smith KM (1974) Plant Viruses. Great Britain Northumberland Press.Google Scholar
  40. 40.
    Van Regenmortel MHV (1982) Serology and immunochemistry of plant viruses. Academic Press, New York.Google Scholar
  41. 41.
    Van Regenmortel MHV (1985) New serological procedures including the development and uses of monoclonal antibodies in virus detection and diagnosis. Acta Horticulturae 164: 187–194.Google Scholar
  42. 42.
    Van Regenmortel MHV (1986) The potential for using monoclonal antibodies in the detection of plant viruses. In: Developments in Applied Biology 1. Developments and Applications in Virus Testing. Eds RAC Jones and L Torrance. Association of Applied Biologists, Wellesbourne, UK.Google Scholar
  43. 43.
    Walkey DGA (1980) Production of virus-free plants by tissue culture. In: Tissue culture methods for plant pathologists (DS Ingram and JP Helgeson, Eds), 109–117. Blackwell Scientific Publications, Oxford.Google Scholar
  44. 44.
    Welvaert W & Samyn G (1985) Relative importance of Pelargonium viruses in cutting nurseries. Acta Horticulturae 164: 195–198.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1992

Authors and Affiliations

  • Mary C. Coleman
    • 1
  • Wayne Powell
    • 2
  1. 1.Agricultural Genetics Company Ltd.CambridgeUK
  2. 2.Scottish Crop Research InstituteInvergowrie, DundeeScotland

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