Cloning and Characterisation of a Mosquito Acetylcholinesterase Gene
Acetylcholinesterase (AChE, EC 188.8.131.52), a serine hydrolase, catalyzes the breakdown of the neurotransmitter acetylcholine into acetate and choline. This involves the formation of a substrate enzyme complex, followed by acetylation of the hydroxyl group of the amino acid serine, present within the esteratic site, and finally deacetylation. AChE is the target site for carbamate and organophosphate (OP) insecticides. These compounds react in an analogous way to acetylcholine, forming a complex, then respectively carbamylating or phosphorylating the enzyme. The dephosphorylating and decarbamylating steps are however very slow thus inhibiting the enzyme. (O’Brian 1976).
KeywordsInsecticide Resistance Polytene Chromosome Salivary Gland Chromosome Larval Salivary Gland EMBO Journal
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