Isolation, Characterization and Progress in Cloning of Cyclodiene Insecticide Resistance in Drosophila melanogaster

  • R. H. ffrench-Constant
  • R. T. Roush
  • R. J. MacIntyre


Insecticide resistance is an increasingly serious problem for the control of medically and agriculturally important pests. More than 447 species of arthropods have evolved resistance to one or more pesticides; some 17 species have shown resistance to all five major classes of insecticides often outstripping the rate at which new pesticides (or even non-pesticidal controls) can be introduced to replace them (Georghiou 1986). Insecticide resistance is also a dramatic example of adaptation at the molecular level. The majority of cases of practically significant resistance in the field are due to single major genes (Roush & McKenzie 1987, Roush and Daly 1990), that either increase the metabolism of pesticides or cause insensitivity in the target site (Soderlund &Bloomquist 1990).


Insecticide Resistance Scintillation Vial Musca Domestica Mixed Function Oxidase Organophosphorus Insecticide 
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  1. Bender, W., Spierer, P. and Hogness, D.S. 1983. Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster. J. Mol. Biol. 168: 17–33.PubMedCrossRefGoogle Scholar
  2. Berge, J.B. and Fournier, D. 1988. Advances in molecular genetics of acetylcholinesterase insensitivity in insecticide-resistant insects, p. 461 in: “Procedings XVIII International Congress of Entomology.” Vancouver.Google Scholar
  3. Dillela, A.G. and Woo S.L.C. 1985. Cosmid cloning of genomic DNA. Focus (Bethesda Research Laboratories) Vol. 7(2): 5.Google Scholar
  4. Eldefrawi, A.T. and Eldefrawi, M.E. 1987. Receptors for γ-aminobutyric acid and voltage-dependent chloride channels as targets for drugs and toxicants. FASEB J. 1: 262–271.PubMedGoogle Scholar
  5. Feyereisen, R., Koener, J.F., Farnsworth, D.E. and Nebert, D.W. 1989. Isolation and sequence of cDNA encoding a cytochrome P-450 from an insecticide-resistant strain of the house fly, Musca domestica. Proc. Natl. Acad. Sci. USA. 86: 1465–1469.PubMedCrossRefGoogle Scholar
  6. ffrench-Constant, R.H. and Roush, R.T. 1990. Gene mapping and cross-resistance in cyclodiene insecticide resistant Drosophila melanogaster. Genebical Research. In press.Google Scholar
  7. ffrench-Constant, R.H., Roush, R.T., Mortlock, D. and Dively, G.P. 1990. Isolation of dieldrin resistance from field populations of Drosophila melanogaster. J. Econ. Entomol. In press.Google Scholar
  8. Field, L.M., Devonshire, A.L. and Forde, B.G. 1988. Molecular evidence that insecticide resistance in peach-potato aphids (Myzus persicae) results from amplification of an esterase gene. Biochem. J. 251: 309–312.PubMedGoogle Scholar
  9. Foster, G.G., Whitten, M.J., Konovalov, C., Arnold, J.T.A. and Maffi, G. 1981. Autosomal genetic maps of the Australian sheep blowfly, Lucilia cuprina dorsalis R-D. and possible correlations with the linkage maps of Musca domestica and Drosophila melanogaster. Genet. Res. 37: 55–69.CrossRefGoogle Scholar
  10. Georghiou, G.P. 1986. The magnitude of the resistance problem. pp. 14–43 in: “Pesticide resistance: Strategies and tactics for management.” National Academy of Sciences, ed. National Academy Press: Washington, D.C.Google Scholar
  11. Houpt, D.R., Pursey, J.C. and Morton, R.A. 1988. Genes controlling malathion resistance in a laboratory selected population of Drosophila melanogaster. Genome 30: 844–853.PubMedCrossRefGoogle Scholar
  12. Kadous, A.A., Ghiasuddin, S.M., Matsumura, F., Scott, J.G. and Tanaka, K. 1983. Difference in the picrotoxinin receptor between the cyclodiene resistant and susceptible strains of the German cockroach. Pestic. Biochem. Physiol. 19: 157–166.CrossRefGoogle Scholar
  13. Kasbekar, D.P. and Hall, L.M. 1988. A Drosophila mutation that reduces sodium channel number confers resistance to pyrethroid insecticides. Pestic. Biochem. Physiol. 32: 135–145.CrossRefGoogle Scholar
  14. Leicht, B.G. and Bonner, J.J. 1988. Genetic analysis of chromosomal region 67A-D of Drosophila melanogaster. Genetics. 119: 579–593.PubMedGoogle Scholar
  15. Morton, R.A. and Singh, R.S. 1982. The association between malathion resistance and acetylcholinesterase in Drosophila melanogaster. Biochem. Gen. 20: 179–198.CrossRefGoogle Scholar
  16. Oppenoorth, F.J. 1985. Biochemistry and genetics of insecticide resistance, pp. 731–773 in: “Comprehensive Insect Physiology, Biochemistry, and Pharmacology.” vol 12. G.A. Kerkut and L.I. Gilbert, ed. Pergamon, New York.Google Scholar
  17. Pardue, ML. 1986. In situ hybridization to DNA of chromosomes and nuclei. pp. 111–137 in: “Drosophila, A Practical Approach”. D.B. Roberts, ed. IRL Press, Oxford, England.Google Scholar
  18. Roush, R.T. and Daly, J. 1990. The role of population genetics in resistance research and management. in: “Pesticide Resistance in Arthropods.” Roush, R.T. and Tabashnik, B.E., eds. Chapman and Hall, New York. in press.Google Scholar
  19. Roush, R.T. and McKenzie, J.A. 1987. Ecological genetics of insecticide and acaricide resistance. Ann. Rev. Entomol. 32: 361–380.CrossRefGoogle Scholar
  20. Schofield, P.R., Darlison, M.G., Fugita, N., Burt, D.R., Stephenson, F.A., Rodriguez, H., Rhee, L.M., Ramachandran, J., Reale, V., Glencorse, T.A., Seeburg, P. H. and Barnard, E. A. 1987. Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-family. Nature 328: 221–227.PubMedCrossRefGoogle Scholar
  21. Soderlund, D.M. and Bloomquist, J.R. 1990. Molecular mechanisms of insecticide resistance. in: “Pesticide Resistance in Arthropods.” Roush, R.T. and Tabashnik, B.E., eds. Chapman and Hall, New York. in press.Google Scholar
  22. Tanaka, K. 1987. Mode of action of compounds acting at inhibitory synapse. J. Pestic. Sci. 12: 549–560.CrossRefGoogle Scholar
  23. Waters, L.C. and Nix, C.E. 1988. Regulation of insecticide resistance-related cytochrome P-450 expression in Drosoohila melanogaster. Pestic. Biochem. Physiol. 30: 214–227.CrossRefGoogle Scholar
  24. Wilson, T.G. 1988. Drosoohila melanogaster: A model insect for insecticide resistance studies. J. Econ. Entomol. 81: 22–27.PubMedGoogle Scholar
  25. Wilson, T.G. and Fabian, J. 1986. A Drosophila melanogaster mutant resistant to a chemical analog of juvenile hormone. Developmental Biology 118: 190–201.PubMedCrossRefGoogle Scholar
  26. Yarbrough, J.D., Roush, R.T., Bonner, J.C and Wise, D.A. 1986. Monogenic inheritance of cyclodiene resistance in mosquito fish, Gambusia affinis. Experientia 42: 851–853.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • R. H. ffrench-Constant
    • 1
  • R. T. Roush
    • 1
  • R. J. MacIntyre
    • 2
  1. 1.Department of EntomologyComstock HallIthacaUSA
  2. 2.Section of Genetics and Development, Biotechnology BuildingCornell UniversityIthacaUSA

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