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Structure, Expression, and Evolution of the D. melanogaster DPKQDFMRF-Amide Neuropeptide Gene

  • P. H. Taghert
  • L. E. Schneider
  • M. A. O’Brien

Abstract

In recent years, there has been a tremendous increase in the amount of information detailing the structure and expression of biologically active neuropeptides. While we have exact knowledge of many neuropeptides, the promise of a better understanding of what they do, both as individuals and as a class, and of how their diversity improves neuronal function, remains largely unfulfilled. Because of its diminutive size, Drosophila melanogaster has not been a favored subject for physiological studies. The study of neuropeptide expression and function in D. melanogaster is highly attractive, however, because of the advanced genetics and molecular techniques that are available for studies of gene regulation and function in vivo. The functions of neuropeptides are often difficult to analyze because of their structural diversity and because of a lack of specific antagonists. However, in D. melanogaster, the ability to create gene mutations offers the possibility of examining animals that chronically lack specific neuropeptide gene expression. Furthermore, the ability to transform the D. melanogaster germ line via P elements (Rubin and Spradling, 1982) means that such expression could be re-introduced to the nervous system, and in this way, neuropeptide function may be defined by the rescue of mutant phenotypes. With these ideas in mind, we initiated experiments to describe a D. melanogaster neuropeptide gene, its organization and its expression, as starting points from which to undertake a genetic analysis.

Keywords

Favored Subject Hydrophobic Leader Sequence Cell Body Position Dopa Decarboxylase Gene FMRFamide Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Beall, C.J. and Hirsch, J. 1987. Regulatiion of the D. melanogaster Dopa decarboxylase gene in neuronal and non-neuronal cells. Genes and Devel. 1: 510–520.CrossRefGoogle Scholar
  2. Blackman, R.K. and Meselson, M. 1986. Inter-specific nucleotide sequence comparisons used to identify regulatory and structural features of the D. melanogaster hsp82 gene. J. Mol. Biol. 188: 499–515.PubMedCrossRefGoogle Scholar
  3. Haeuptle, M.-T., Flint, N., Gough, N.M. and Dobberstein, B. 1989. A tripartite structure of the signals that determine protein insertion into the endoplasmic reticulum membrane. J. Cell. Biol. 108: 1227–1236.PubMedCrossRefGoogle Scholar
  4. Johnson, W.A., McCormick, C.A., Bray, S.J. and Hirsch, J. 1989. A neuron-specific enhancer of the Drosophila melanogaster dopa decarboxylase gene. Genes and Develop.. in press.Google Scholar
  5. Nambu, J.R., Murphy-Erdosh, C., Andrews, P.C., Feistner, G. and Scheller, R.H. 1988. Isolation and characterization of a Drosophila melanogaster neuropeptide gene. Neuron 1: 55–61.PubMedCrossRefGoogle Scholar
  6. Nüsslein-Vollhard, C., Weischaus, E. and Kluding, H. 1984. Mutations affecting the pattern of the larval cuticle in Drosophila melanogaster. I. Zygotic loci on the second chromosome. Roux’s Arch. Devel. Biol. 193: 267–282.Google Scholar
  7. Price, D.A. 1986. Evolution of a molluscan cardioregulatory neuropeptide. Amer. Zool. 26: 1007–1017.Google Scholar
  8. Price, D.A., Greenburg, M.J. 1977. Structure of a molluscan cardioacceleratory peptide. Science 197: 670–671.PubMedCrossRefGoogle Scholar
  9. Rosenfeld, M.G., Amara, S.G. and Evans, R.M. 1984. Alternative RNA processing: determining neuronal phenotype. Science 225: 1315–1320.PubMedCrossRefGoogle Scholar
  10. Rubin, G.M. and Spradling, A. 1982. Genetic transformation of Drosophila melanogaster with transposable element vectors. Science 218: 348–353.PubMedCrossRefGoogle Scholar
  11. Scheller, R.H., Khaldany, R.R., Kreiner, T., Mahon, A.C., Nambu, J.R., Schaeffer, M. and Taussig, R. 1984. Neuropeptides: Mediators of behavior in Aplysia. Science 225: 1300–1308.PubMedCrossRefGoogle Scholar
  12. Schneider, L.E., and Taghert, P.H. 1988. Isolation and characterization of a Drosophila melanogaster gene encoding multiple neuropeptides related to FMRFamide (Phe-Met- Arg-Phe-NH2). Proc. Nad. Acad. Sci. USA 85: 1993–1997.CrossRefGoogle Scholar
  13. Schneider, L.E., and Taghert, P.H. 1990. Organization and expression of the Drosophila Phe-Met-Arg-Phe-amide neuropeptide gene. J. Biol. Chem. In press.Google Scholar
  14. Taghert, P. H. and Schneider, L. E. Interspecific comparison of a Drosophila neuropeptide gene encoding FMRF-amide-related peptide. J. Neurosci. in press.Google Scholar
  15. Taussig, R. and Scheller, R.H. 1986. The Aplysia FMRFamide gene encodes sequences related to mammalian brain peptides. DNA 5: 453–461.PubMedCrossRefGoogle Scholar
  16. Throckmorton, L.H. 1975. pp 421–469 in: “Handbook of Genetics.” Vol.3. King, R.C,. ed. Plenum Press, New York.Google Scholar
  17. Treier, M., Pfeifle, C. and Tautz, D. 1989. Comparison of the gap segmentation gene hunchback between Drosophila melanogaster and D. virilis reveals novel modes of evolutionary change. EMBO J. 8: 1517–1525.PubMedGoogle Scholar
  18. Trimmer, B.A., Kobierski, L.A. and Kravitz, E.A. 1987. Purification and characterization of FMRFamide-like immunoreactive substances from the lobster nervous system: Isolation and sequence analysis of two closely related peptides. J. Comp. Neurol. 266: 16–26.PubMedCrossRefGoogle Scholar
  19. Truman, J.W. and Bate, C.M. 1989. Spatial and temporal patterns of neurogenesis in the central nervous system of Drosophila melanogaster. Devel. Biol. 125: 145–157.CrossRefGoogle Scholar
  20. Vale, W.W., Rivier, C., Spiess, J. and Rivier, J. 1983. pp. 67–73 in: “Brain Peptides.” Dreiger, D., Brownstein, M.J. and Martin, J.B., eds. Wiley, New York.Google Scholar
  21. von Heijne, G. 1983. Patterns of amino acids near signal-sequence cleavage sites. EMBO J. 5: 3021–3027.Google Scholar
  22. Yang, H.-Y.T., Fratta, W., Majane, E.A. and Costa, E. 1985. Isolation, sequencing, synthesis and pharmacological characterization of two rat brain neuropeptides that modulate the actions of morphine. Proc. Nat. Acad. Sci. USA 82: 7757–7761.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • P. H. Taghert
    • 1
  • L. E. Schneider
    • 1
  • M. A. O’Brien
    • 1
  1. 1.Department of Anatomy and NeurobiologyWashington University School of MedicineSt. LouisUSA

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