Identification and Characterization of a Baculovirus Ecdysteroid UDP-Glucosyl Transferase Gene
Control of ecdysteroid levels is of paramount importance to the regulation of insect development. In addition to control at the levels of biosynthesis and secretion, regulation of ecdysteroid metabolism is also critical for the maintenance of appropriate concentrations of these hormones. The metabolism of ecdysteroids is highly complex, and many different classes of metabolic transformation are known to occur (Koolman and Karlson, 1984). The relative importance of such reactions to the inactivation of ecdysteroids is being elucidated only now. The identification of the genes encoding enzymes involved in ecdysteroid metabolism will greatly facilitate this task. In this manuscript, the characterization of a gene (egt) encoding a glucosyl transferase specific for ecdysteroids is described.
KeywordsSF21 Cell Glucuronic Acid Nuclear Polyhedrosis Virus Glucosyl Transferase Tissue Culture Fluid
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