Abstract
Control of ecdysteroid levels is of paramount importance to the regulation of insect development. In addition to control at the levels of biosynthesis and secretion, regulation of ecdysteroid metabolism is also critical for the maintenance of appropriate concentrations of these hormones. The metabolism of ecdysteroids is highly complex, and many different classes of metabolic transformation are known to occur (Koolman and Karlson, 1984). The relative importance of such reactions to the inactivation of ecdysteroids is being elucidated only now. The identification of the genes encoding enzymes involved in ecdysteroid metabolism will greatly facilitate this task. In this manuscript, the characterization of a gene (egt) encoding a glucosyl transferase specific for ecdysteroids is described.
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References
Dutton, G. 1980. “Glucuronidation of Drugs and Other Compounds.” CRC Press, Boca Raton.
Heinrich, G. and Hoffmeister, H. 1970 Insect molting hormones and their mode of action. Formation of hormone glycosides as an inactivation mechanism in Calliphora erythrocephala. Z. Naturf. B. 25: 358–361.
Jackson, M.R. and Burchell, B.R. 1986. The full length coding sequence of rat liver androsterone UDP-glucuronyltransferase cDNA and comparison with other members of this gene family. Nucleic Acids Res. 14: 779–795.
Jackson, M.R., McCarthy, L.R., Harding, D., Wilson, S., Coughtrie, M.W.H. and Burchell, B. 1987. Cloning of a human liver microsomal UDP-glucuronosyltransferase cDNA. Biochem. J. 242: 581–588.
Kimura, T. and Owens, I.S. 1987. Mouse UDP-glucuronosyltransferase. cDNA and complete amino acid sequence and regulation. Eur. J. Biochem. 168: 515–521.
King, D.S. 1972. Metabolism of α-ecdysone and possible intermediate precursors by insects in vivo and in vitro. Gen. Comp. Endocrinol. Suppl. 3: 221–227.
Koolman, J. and Karlson, P. 1984. Regulation of ecdysteroid titer: degradation. pp 343–362 in: “Comprehensive Insect Physiology Biochemistry and Pharmacology.” Vol. 7. Kerkut, G. A. and Gilbert, L.I., eds. Pergamon Press, Oxford.
Kumar, S. and Miller, L.K. 1987. Effects of serial passage of Autographa californica nuclear polyhedrosis virus in cell culture. Virus Res. 7: 335–349.
Mackenzie, P.I. 1987. Rat liver UDP-glucuronosyltransferase. Identification of cDNAs encoding two enzymes which glucuronidate testosterone, dihydrotestosterone, and β-estradiol. J. Biol. Chem. 262: 9744–9749.
Nillson, T, Jackson, M. and Peterson, P.A. 1989. Short cytoplasmic sequences serve as retention signals for transmembrane proteins in the endoplasmic reticulum. Cell 58: 707–718.
O’Reilly, D.R. and Miller, L.K. 1989. A baculovirus blocks insect molting by producing ecdysteroid UDP-glucosyl transferase. Science 245: 1110–1112.
O’Reilly, D.R. and Miller, L.K. 1990 Regulation of expression of a baculovirus ecdysteroid UDP-glucosyl transferase gene. J.Virol. 64: 1321–1328.
Ralston, E.J., English, J.J. and Dorner, H.K. 1988. Sequence of three bronze alleles of maize and correlation with the genetic fine structure. Genetics 119: 185–197.
Smith, J.N. 1977. Comparative detoxication of invertebrates. pp. 219–232 in: “Drug Metabolism — from Microbe to Man.” Parke, D.V. and Smith, R.L., eds. Taylor and Francis, London.
Thompson, M.J., Feldlaufer, M.F., Lozano, R., Rees, H.H., Lusby, W.R., Svoboda, J.A. and Wilzer, KR. 1987. Metabolism of 26-[14C]hydroxyecdysone 26-phosphate in the tobacco hornworm Manduca sexta L., to a new ecdysteroid conjugate: 26- [14C]hydroxyecdysone 22-glucoside. Arch. Insect Biochem. Physiol. 4: 1–15.
Thompson, M.J., Svoboda, J.A., Lozano, R. and Wilzer, K.R. 1988. Profile of free and conjugated ecdysteroids and ecdysteroid acids during embryonic development of Manduca sexta (L.) following maternal incorporation of [14C]cholesterol. Arch. Insect Biochem. Physiol. 7: 157–172.
Warren, J.T., Steiner, B, Dorn, A., Pak, M. and Gilbert, L.I. 1986. Metabolism of ecdysteroids during the embryogenesis of Manduca sexta. J. Liq. Chromatog. 9: 1759–1782.
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O’Reilly, D.R. (1990). Identification and Characterization of a Baculovirus Ecdysteroid UDP-Glucosyl Transferase Gene. In: Hagedorn, H.H., Hildebrand, J.G., Kidwell, M.G., Law, J.H. (eds) Molecular Insect Science. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3668-4_13
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DOI: https://doi.org/10.1007/978-1-4899-3668-4_13
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