Role of Platelet Activating Factor in the Adhesion Process of Polymorphonuclear Neutrophils to Endothelial Cells

  • Federico Bussolino
  • Daniela Alessi
  • Ernesto Turello
  • Giovanni Camussi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 297)


Circulating polymorphonuclear neutrophils (PMN) provide a front line of defense that can be rapidly mobilized and activated against infectious and toxic agents. The first step in extravasion involves the adhesion of neutrophils to vascular endothelium. This process must be regulated to allow localization of neutrophils only to inflammatory sites. Chemotactic factors, cytokines or lipid mediators released at the inflamed sites may modify the characteristics of plasma-membrane surface of PMN, endothelial cells (EC) or of both and promote PMN-EC adhesion (reviewed in 1). For example, the leukocyte CD11/CD18 surface adhesive complex and its ligand (intercellular adhesion molecule 1) on EC surface, are essential in the PMN extravasion process and their expression can be up-regulated by cytokines and chemotactic peptides (2–4). Interleukin-1 and tumor necrosis factor induce a transient expression of the glycoprotein endothelial-leukocyte adhesion molecule-1 on EC surface that mediate PMN-EC interaction (4,5).


Platelet Activate Factor Adhesion Assay Polymorphonuclear Neutrophil Endothelial Cell Surface Chemotactic Peptide 
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  1. 1.
    Harlam, J.M.: Leukocyte-endothelial interactions. Blood 65:513 (1985).Google Scholar
  2. 2.
    Kishimoto, T.K., Jutila, M.A., Berg, E.L., Butcher, E.C.: Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotatic factors. Science 245:1238 (1989).PubMedCrossRefGoogle Scholar
  3. 3.
    Smith, C.W., Rothlein, R., Hughes, B.J., Mariscalco, M.M., Rudioff, H.E., Schmalstieg, F.C., Anderson, D.C.: Recognition of an endothelial determinant for CD18-dependent human neutrophil adherence and transendothelial migration. J. Clin. Invest. 82:1746 (1988).PubMedCrossRefGoogle Scholar
  4. 4.
    Luscinskas, F.W., Brock, F.A., Arnaut, M.A., Gimbrone, M.A.: Endothelial-leukocyte adhesion molecule-1-dependent and leukocyte (CDU/CD18)-dependent mechanisms contribute to polymorphonuclear leukocyte adhesion to cytokine-activated human vascular endothelium. J. Immunol. 142:2257 (1989).PubMedGoogle Scholar
  5. 5.
    Bevilacqua, M.P., Pober, J.S., Mendrick, D.L., Cotran, R.S., Gimbrone, M.A.: Identification of an inducible endothelial-leukocyte-adhesion molecule. Proc. Natl. Acad. Sci. USA 84:9238 (1987).PubMedCrossRefGoogle Scholar
  6. 6.
    Braquet, P., Touqui, L., Shen, T.Y., Vargaftig, B.B.: Perspective in platelet activating factor research. Pharmacol. Rev. 39:97 (1987).PubMedGoogle Scholar
  7. 7.
    Tonnesen, M.G., Anderson, C.A., Spriner, T.A., Knedler, A., Avdi, N., Henson, P.M.: Adherence of neutrophils to cultured human microvascular endothelial cells. Stimulation by chemotactic peptides and lipid mediators and dependence upon the Mac-1, LFA-1 and p150,95 glyoprotein family. J. Clin. Invest. 83:637 (1989).PubMedCrossRefGoogle Scholar
  8. 8.
    Kimani, G., Tonnesen, M.G., Henson, P.M.: Stimulation of eosinophils adherence to human vascular endothelial cells in vitro by platelet activating factor. J. Immunol. 140:3161 (1989).Google Scholar
  9. 9.
    Valone, F.H., Goetzl, E.J.: Enhancement of human polymorphonuclear leukocyte adherence by the phospholipid mediator l-0-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC). Am. J. Pathol. 113:85 (1983).PubMedGoogle Scholar
  10. 10.
    Zimmerman, A.G., McIntyre, T.M., Prescott, S.M.: Thrombin stimulates the adherence of neutrophils to human endothelial cells in vitro. J. Clin. Invest. 76:2235 (1985).PubMedCrossRefGoogle Scholar
  11. 11.
    Camussi, G., Bussolino, F., Salvidio, G., Baglioni, C.: Tumor necrosis factor/cachectin stimulates peritoneal macrophages, polymorphonuclear neutrophils and vascular endothelial cells to synthesize and release platelet activating factor. J. Exp. Med. 166:1390 (1987).PubMedCrossRefGoogle Scholar
  12. 12.
    Breviario, F., Bertocchi, F., Dejana, E., Bussolino, F.: IL-1-induced adhesion of polymorphonuclear leukocytes to cultured human endothelial cells. Role of platelet-activating factor. J. Immunol. 141:3391 (1988).PubMedGoogle Scholar
  13. 13.
    Bussolino, F., Gremo, F., Pescarmona, G.P., Camussi, G.: Platelet activating factor generation from chick retina. J. Biol. Chem. 261:16502 (1986).PubMedGoogle Scholar
  14. 14.
    Braquet, P., Paubert-Braquet, M., Bourgain, R.H., Bussolino, F., Hosford, D.: PAF/cytokine autogenerated feedback networks in microvascular immune injury: consequences in shock, ischemia and graft rejection. J. Lipid Mediat. 1:75 (1989).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Federico Bussolino
    • 1
    • 2
  • Daniela Alessi
    • 1
    • 2
  • Ernesto Turello
    • 1
    • 2
  • Giovanni Camussi
    • 1
    • 2
  1. 1.Dipartimento di Genetica, Biologia e Chimica Medica, Laboratorio di ImmunopatologiaUniversita’ di TorinoItaly
  2. 2.Dipartimento di Biochimica e BiofisicaUniversita’ di NapoliItaly

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