Role of Platelet Activating Factor in the Adhesion Process of Polymorphonuclear Neutrophils to Endothelial Cells
Circulating polymorphonuclear neutrophils (PMN) provide a front line of defense that can be rapidly mobilized and activated against infectious and toxic agents. The first step in extravasion involves the adhesion of neutrophils to vascular endothelium. This process must be regulated to allow localization of neutrophils only to inflammatory sites. Chemotactic factors, cytokines or lipid mediators released at the inflamed sites may modify the characteristics of plasma-membrane surface of PMN, endothelial cells (EC) or of both and promote PMN-EC adhesion (reviewed in 1). For example, the leukocyte CD11/CD18 surface adhesive complex and its ligand (intercellular adhesion molecule 1) on EC surface, are essential in the PMN extravasion process and their expression can be up-regulated by cytokines and chemotactic peptides (2–4). Interleukin-1 and tumor necrosis factor induce a transient expression of the glycoprotein endothelial-leukocyte adhesion molecule-1 on EC surface that mediate PMN-EC interaction (4,5).
KeywordsPlatelet Activate Factor Adhesion Assay Polymorphonuclear Neutrophil Endothelial Cell Surface Chemotactic Peptide
Unable to display preview. Download preview PDF.
- 1.Harlam, J.M.: Leukocyte-endothelial interactions. Blood 65:513 (1985).Google Scholar
- 4.Luscinskas, F.W., Brock, F.A., Arnaut, M.A., Gimbrone, M.A.: Endothelial-leukocyte adhesion molecule-1-dependent and leukocyte (CDU/CD18)-dependent mechanisms contribute to polymorphonuclear leukocyte adhesion to cytokine-activated human vascular endothelium. J. Immunol. 142:2257 (1989).PubMedGoogle Scholar
- 7.Tonnesen, M.G., Anderson, C.A., Spriner, T.A., Knedler, A., Avdi, N., Henson, P.M.: Adherence of neutrophils to cultured human microvascular endothelial cells. Stimulation by chemotactic peptides and lipid mediators and dependence upon the Mac-1, LFA-1 and p150,95 glyoprotein family. J. Clin. Invest. 83:637 (1989).PubMedCrossRefGoogle Scholar
- 8.Kimani, G., Tonnesen, M.G., Henson, P.M.: Stimulation of eosinophils adherence to human vascular endothelial cells in vitro by platelet activating factor. J. Immunol. 140:3161 (1989).Google Scholar